Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              18    1135
XP_006715292                                                           18    1135
NP_001957                                                              16    5631
NP_001159887                                                           16    5631
NP_003250                                                              16    5631
XP_006721993                                                           16    5631
XP_006713588                                                           16    5631
NP_057085                                                              16    5631
NP_004045                                                              16    7355
XP_005259159                                                           16    7355
NP_065913                                                              16    7355
NP_683692                                                              15    9605
NP_056381                                                              15    9605
XP_005248537                                                           15    9605
XP_005248536                                                           15    9605
XP_005248208                                                           15    9605
XP_005248207                                                           15    9605
XP_006714021                                                           15    9605
>NP_115927
          Length = 1321

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DAFLSTSTS 9
           DA LSTSTS
Sbjct: 316 DALLSTSTS 324
>XP_006715292
          Length = 759

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DAFLSTSTS 9
           DA LSTSTS
Sbjct: 303 DALLSTSTS 311
>NP_001957
          Length = 723

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DAFLSTSTS 9
           +AFL T+TS
Sbjct: 403 EAFLCTNTS 411
>NP_001159887
          Length = 627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DAFLSTSTS 9
           +AFL T+TS
Sbjct: 307 EAFLCTNTS 315
>NP_003250
          Length = 924

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   DAFLSTSTS 9
           DAF++T+T+
Sbjct: 280 DAFVATATA 288
>XP_006721993
          Length = 551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FLSTSTS 9
           F+STSTS
Sbjct: 241 FISTSTS 247
>XP_006713588
          Length = 515

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DAFLSTSTS 9
           +AFL T+TS
Sbjct: 195 EAFLCTNTS 203
>NP_057085
          Length = 556

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FLSTSTS 9
           F+STSTS
Sbjct: 224 FISTSTS 230
>NP_004045
          Length = 482

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DAFLST 6
           DAFLST
Sbjct: 287 DAFLST 292
>XP_005259159
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DAFLST 6
           DAFLST
Sbjct: 158 DAFLST 163
>NP_065913
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DAFLST 6
           DAFLST
Sbjct: 158 DAFLST 163
>NP_683692
          Length = 3051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AFLSTSTS 9
            A +STSTS
Sbjct: 2291 AMISTSTS 2298
>NP_056381
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DAFLSTS 7
           DAF STS
Sbjct: 310 DAFASTS 316
>XP_005248537
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DAFLSTS 7
           DAF STS
Sbjct: 310 DAFASTS 316
>XP_005248536
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DAFLSTS 7
           DAF STS
Sbjct: 372 DAFASTS 378
>XP_005248208
          Length = 3097

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AFLSTSTS 9
            A +STSTS
Sbjct: 2291 AMISTSTS 2298
>XP_005248207
          Length = 3098

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AFLSTSTS 9
            A +STSTS
Sbjct: 2291 AMISTSTS 2298
>XP_006714021
          Length = 3112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    AFLSTSTS 9
            A +STSTS
Sbjct: 2291 AMISTSTS 2298
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.107    0.256 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 323,012
Number of extensions: 289
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package