Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              19     869
XP_005266703                                                           19     869
NP_073609                                                              17    3301
XP_006718339                                                           17    3301
XP_006718338                                                           17    3301
XP_006718337                                                           17    3301
XP_006718336                                                           17    3301
NP_110424                                                              16    7355
NP_001135918                                                           16    7355
NP_065208                                                              16    7355
XP_005273533                                                           16    7355
XP_005262687                                                           16    7355
XP_006712171                                                           16    7355
NP_065210                                                              16    7355
NP_065209                                                              16    7355
NP_000028                                                              16    7355

>NP_443179
          Length = 2170

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DAESALAD 8
            DAESALAD
Sbjct: 1302 DAESALAD 1309



>XP_005266703
          Length = 1501

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   DAESALAD 8
           DAESALAD
Sbjct: 633 DAESALAD 640



>NP_073609
          Length = 715

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   DAESALAD 8
           D ESALAD
Sbjct: 166 DIESALAD 173



>XP_006718339
          Length = 546

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   DAESALAD 8
           D ESALAD
Sbjct: 166 DIESALAD 173



>XP_006718338
          Length = 715

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   DAESALAD 8
           D ESALAD
Sbjct: 166 DIESALAD 173



>XP_006718337
          Length = 715

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   DAESALAD 8
           D ESALAD
Sbjct: 166 DIESALAD 173



>XP_006718336
          Length = 715

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   DAESALAD 8
           D ESALAD
Sbjct: 166 DIESALAD 173



>NP_110424
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  DAESALAD 8
          D+ES LAD
Sbjct: 44 DSESILAD 51



>NP_001135918
          Length = 1897

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DAESALADR 9
            +AES+ ADR
Sbjct: 1808 EAESSQADR 1816



>NP_065208
          Length = 1856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DAESALADR 9
            +AES+ ADR
Sbjct: 1767 EAESSQADR 1775



>XP_005273533
          Length = 1921

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DAESALADR 9
            +AES+ ADR
Sbjct: 1808 EAESSQADR 1816



>XP_005262687
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  DAESALAD 8
          D+ES LAD
Sbjct: 44 DSESILAD 51



>XP_006712171
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  DAESALAD 8
          D+ES LAD
Sbjct: 44 DSESILAD 51



>NP_065210
          Length = 1719

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DAESALADR 9
            +AES+ ADR
Sbjct: 1605 EAESSQADR 1613



>NP_065209
          Length = 1881

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DAESALADR 9
            +AES+ ADR
Sbjct: 1767 EAESSQADR 1775



>NP_000028
          Length = 1880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DAESALADR 9
            +AES+ ADR
Sbjct: 1767 EAESSQADR 1775



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.115    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 291,998
Number of extensions: 403
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)