Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              21     175
NP_001243599                                                           17    2528
NP_569710                                                              17    2528
NP_005691                                                              17    2528
NP_789792                                                              16    5631
NP_055907                                                              16    5631
XP_005255242                                                           16    5631
XP_005255241                                                           16    5631
XP_005255240                                                           16    5631
XP_005255239                                                           16    5631
XP_006718281                                                           16    5631
NP_085128                                                              16    7355
NP_038203                                                              16    7355
NP_079229                                                              16    7355
NP_001128119                                                           16    7355
NP_001091966                                                           16    7355
NP_001091963                                                           16    7355
NP_001091962                                                           16    7355
NP_001091961                                                           16    7355
NP_078967                                                              16    7355
NP_002152                                                              16    7355
NP_940944                                                              16    7355
XP_005258937                                                           16    7355
XP_005258935                                                           16    7355
XP_005253995                                                           16    7355
XP_006719669                                                           16    7355
XP_006713973                                                           16    7355
XP_006713971                                                           16    7355
NP_001091965                                                           16    7355
NP_001091964                                                           16    7355
NP_079390                                                              15    9605
NP_002833                                                              15    9605
NP_001154912                                                           15    9605
NP_689961                                                              15    9605
NP_001136221                                                           15    9605
NP_065777                                                              15    9605
NP_036337                                                              15    9605
NP_060613                                                              15    9605
XP_006723375                                                           15    9605
XP_006719673                                                           15    9605
XP_005269218                                                           15    9605
XP_005269217                                                           15    9605
XP_006719672                                                           15    9605
XP_005269215                                                           15    9605
XP_006715958                                                           15    9605
XP_006715957                                                           15    9605
XP_006714724                                                           15    9605
XP_005248616                                                           15    9605
XP_005264287                                                           15    9605
>NP_004110
          Length = 993

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DAEEVMYQN 9
           DAEE MYQN
Sbjct: 949 DAEEAMYQN 957
>NP_001243599
          Length = 707

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DAEEVMYQN 9
           DAE+V+Y N
Sbjct: 504 DAEDVIYVN 512
>NP_569710
          Length = 737

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DAEEVMYQN 9
           DAE+V+Y N
Sbjct: 534 DAEDVIYVN 542
>NP_005691
          Length = 737

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DAEEVMYQN 9
           DAE+V+Y N
Sbjct: 534 DAEDVIYVN 542
>NP_789792
          Length = 1093

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EEVMYQ 8
           EE+MYQ
Sbjct: 245 EEIMYQ 250
>NP_055907
          Length = 3661

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DAEEVMYQN 9
            D E+V Y+N
Sbjct: 3442 DGEDVPYEN 3450
>XP_005255242
          Length = 3609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DAEEVMYQN 9
            D E+V Y+N
Sbjct: 3390 DGEDVPYEN 3398
>XP_005255241
          Length = 3637

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DAEEVMYQN 9
            D E+V Y+N
Sbjct: 3418 DGEDVPYEN 3426
>XP_005255240
          Length = 3663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DAEEVMYQN 9
            D E+V Y+N
Sbjct: 3444 DGEDVPYEN 3452
>XP_005255239
          Length = 3689

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DAEEVMYQN 9
            D E+V Y+N
Sbjct: 3470 DGEDVPYEN 3478
>XP_006718281
          Length = 983

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EEVMYQ 8
           EE+MYQ
Sbjct: 135 EEIMYQ 140
>NP_085128
          Length = 2136

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  DAEEVMYQN 9
          D  EV++QN
Sbjct: 85 DRTEVLFQN 93
>NP_038203
          Length = 1262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVMYQN 9
           E+MYQN
Sbjct: 778 ELMYQN 783
>NP_079229
          Length = 972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAEEVMYQ 8
           D EE+M+Q
Sbjct: 347 DPEELMFQ 354
>NP_001128119
          Length = 814

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   EEVMYQN 9
           EEV+Y N
Sbjct: 527 EEVLYDN 533
>NP_001091966
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 33 DAQRLLYRN 41
>NP_001091963
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 33 DAQRLLYRN 41
>NP_001091962
          Length = 498

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 34 DAQRLLYRN 42
>NP_001091961
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 47 DAQRLLYRN 55
>NP_078967
          Length = 510

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 46 DAQRLLYRN 54
>NP_002152
          Length = 1262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVMYQN 9
           E+MYQN
Sbjct: 778 ELMYQN 783
>NP_940944
          Length = 442

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 34 DAQRLLYRN 42
>XP_005258937
          Length = 117

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 34 DAQRLLYRN 42
>XP_005258935
          Length = 441

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 33 DAQRLLYRN 41
>XP_005253995
          Length = 836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAEEVMYQ 8
           D EE+M+Q
Sbjct: 211 DPEELMFQ 218
>XP_006719669
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAEEVMYQ 8
           D EE+M+Q
Sbjct: 319 DPEELMFQ 326
>XP_006713973
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   EEVMYQN 9
           EEV+Y N
Sbjct: 548 EEVLYDN 554
>XP_006713971
          Length = 835

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   EEVMYQN 9
           EEV+Y N
Sbjct: 548 EEVLYDN 554
>NP_001091965
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 34 DAQRLLYRN 42
>NP_001091964
          Length = 478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  DAEEVMYQN 9
          DA+ ++Y+N
Sbjct: 47 DAQRLLYRN 55
>NP_079390
          Length = 2479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    DAEEVMYQN 9
            D EE+ Y N
Sbjct: 1368 DKEEIKYLN 1376
>NP_002833
          Length = 1115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    DAEEVMYQN 9
            D E ++Y+N
Sbjct: 1096 DVENLIYEN 1104
>NP_001154912
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   DAEEVMYQN 9
           D E ++Y+N
Sbjct: 918 DVENLIYEN 926
>NP_689961
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   EEVMYQN 9
           EEV+Y N
Sbjct: 823 EEVVYSN 829
>NP_001136221
          Length = 1029

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   EEVMYQN 9
           EEV+Y N
Sbjct: 990 EEVVYSN 996
>NP_065777
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EEVMYQN 9
          EEV Y+N
Sbjct: 81 EEVTYEN 87
>NP_036337
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  DAEEVMYQ 8
          + EEV YQ
Sbjct: 50 EGEEVFYQ 57
>NP_060613
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   DAEEVMYQ 8
           D+EEV+++
Sbjct: 261 DSEEVLFE 268
>XP_006723375
          Length = 1137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    DAEEVMYQN 9
            D E ++Y+N
Sbjct: 1118 DVENLIYEN 1126
>XP_006719673
          Length = 1997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   DAEEVMYQN 9
           D EE+ Y N
Sbjct: 886 DKEEIKYLN 894
>XP_005269218
          Length = 2009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   DAEEVMYQN 9
           D EE+ Y N
Sbjct: 898 DKEEIKYLN 906
>XP_005269217
          Length = 2353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    DAEEVMYQN 9
            D EE+ Y N
Sbjct: 1399 DKEEIKYLN 1407
>XP_006719672
          Length = 2510

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    DAEEVMYQN 9
            D EE+ Y N
Sbjct: 1399 DKEEIKYLN 1407
>XP_005269215
          Length = 2510

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    DAEEVMYQN 9
            D EE+ Y N
Sbjct: 1399 DKEEIKYLN 1407
>XP_006715958
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   EEVMYQN 9
           EEV+Y N
Sbjct: 876 EEVVYSN 882
>XP_006715957
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   EEVMYQN 9
           EEV+Y N
Sbjct: 876 EEVVYSN 882
>XP_006714724
          Length = 681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EEVMYQN 9
          EEV Y+N
Sbjct: 58 EEVTYEN 64
>XP_005248616
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  EEVMYQN 9
          EEV Y+N
Sbjct: 81 EEVTYEN 87
>XP_005264287
          Length = 201

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  DAEEVMYQ 8
          + EEV YQ
Sbjct: 50 EGEEVFYQ 57
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.119    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 563,249
Number of extensions: 898
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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