Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     509
NP_001035957                                                           20     509
XP_006721991                                                           20     509
XP_006721990                                                           20     509
XP_006721989                                                           20     509
XP_005258041                                                           20     509
XP_006721988                                                           20     509
XP_006721987                                                           20     509
XP_006721986                                                           20     509
XP_005258040                                                           20     509
XP_006721985                                                           20     509
NP_998814                                                              17    4312
XP_005269584                                                           17    4312
XP_005269583                                                           17    4312
XP_006717688                                                           17    4312
XP_005269582                                                           17    4312
NP_001124146                                                           16    7355
NP_001210                                                              16    7355
NP_001186602                                                           16    7355
NP_001186601                                                           16    7355
NP_001186600                                                           16    7355
XP_006716114                                                           16    7355
NP_001008405                                                           16    7355
NP_001558                                                              15    9605
XP_006718600                                                           15    9605
XP_006718599                                                           15    9605
XP_006718598                                                           15    9605
XP_006718597                                                           15    9605
XP_005274036                                                           15    9605
XP_005274035                                                           15    9605
>NP_000258
          Length = 2818

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1144 CTVLAMSNL 1152
>NP_001035957
          Length = 2839

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1144 CTVLAMSNL 1152
>XP_006721991
          Length = 1632

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1154 CTVLAMSNL 1162
>XP_006721990
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1154 CTVLAMSNL 1162
>XP_006721989
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1154 CTVLAMSNL 1162
>XP_005258041
          Length = 2836

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1144 CTVLAMSNL 1152
>XP_006721988
          Length = 2846

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1154 CTVLAMSNL 1162
>XP_006721987
          Length = 2849

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1154 CTVLAMSNL 1162
>XP_006721986
          Length = 2854

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1141 CTVLAMSNL 1149
>XP_005258040
          Length = 2857

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1144 CTVLAMSNL 1152
>XP_006721985
          Length = 2867

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    CTVLAMSKL 9
            CTVLAMS L
Sbjct: 1154 CTVLAMSNL 1162
>NP_998814
          Length = 337

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   CTVLAM 6
           CTVLAM
Sbjct: 291 CTVLAM 296
>XP_005269584
          Length = 221

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   CTVLAM 6
           CTVLAM
Sbjct: 175 CTVLAM 180
>XP_005269583
          Length = 221

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   CTVLAM 6
           CTVLAM
Sbjct: 175 CTVLAM 180
>XP_006717688
          Length = 314

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   CTVLAM 6
           CTVLAM
Sbjct: 291 CTVLAM 296
>XP_005269582
          Length = 328

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   CTVLAM 6
           CTVLAM
Sbjct: 282 CTVLAM 287
>NP_001124146
          Length = 315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CTVLAMSK 8
          CT  A+SK
Sbjct: 13 CTAFALSK 20
>NP_001210
          Length = 315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CTVLAMSK 8
          CT  A+SK
Sbjct: 13 CTAFALSK 20
>NP_001186602
          Length = 224

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CTVLAMSK 8
          CT  A+SK
Sbjct: 13 CTAFALSK 20
>NP_001186601
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CTVLAMSK 8
          CT  A+SK
Sbjct: 21 CTAFALSK 28
>NP_001186600
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CTVLAMSK 8
          CT  A+SK
Sbjct: 21 CTAFALSK 28
>XP_006716114
          Length = 348

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CTVLAMSK 8
           C V+A+SK
Sbjct: 312 CIVMALSK 319
>NP_001008405
          Length = 348

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CTVLAMSK 8
           C V+A+SK
Sbjct: 312 CIVMALSK 319
>NP_001558
          Length = 1258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    CTVLAMSK 8
            C+VL M+K
Sbjct: 1121 CSVLQMAK 1128
>XP_006718600
          Length = 1159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    CTVLAMSK 8
            C+VL M+K
Sbjct: 1022 CSVLQMAK 1029
>XP_006718599
          Length = 1159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    CTVLAMSK 8
            C+VL M+K
Sbjct: 1022 CSVLQMAK 1029
>XP_006718598
          Length = 1206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    CTVLAMSK 8
            C+VL M+K
Sbjct: 1143 CSVLQMAK 1150
>XP_006718597
          Length = 1258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    CTVLAMSK 8
            C+VL M+K
Sbjct: 1121 CSVLQMAK 1128
>XP_005274036
          Length = 1280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    CTVLAMSK 8
            C+VL M+K
Sbjct: 1143 CSVLQMAK 1150
>XP_005274035
          Length = 1280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    CTVLAMSK 8
            C+VL M+K
Sbjct: 1143 CSVLQMAK 1150
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.128    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 295,533
Number of extensions: 322
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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