Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1135
NP_671714                                                              18    1135
XP_006715890                                                           18    1135
XP_005250160                                                           18    1135
XP_005250159                                                           18    1135
NP_060004                                                              17    2528
NP_001093582                                                           17    2528
NP_004865                                                              17    3301
NP_001191807                                                           17    3301
NP_004578                                                              17    3301
NP_001036041                                                           17    3301
NP_001092903                                                           17    4312
NP_002463                                                              17    4312
NP_149022                                                              17    4312
NP_775820                                                              17    4312
XP_005273630                                                           17    4312
NP_061158                                                              17    4312
NP_001157718                                                           16    5631
NP_004378                                                              16    7355
NP_001128669                                                           16    7355
NP_055510                                                              16    7355
XP_005256934                                                           16    7355
XP_006721670                                                           16    7355
XP_005265877                                                           16    7355
XP_005265157                                                           16    7355
XP_005265156                                                           16    7355
XP_006713187                                                           16    7355
NP_835467                                                              16    7355
NP_060626                                                              15    9605
NP_056193                                                              15    9605
NP_060921                                                              15    9605
NP_066564                                                              15    9605
NP_001274359                                                           15    9605
NP_001185980                                                           15    9605
NP_001268369                                                           15    9605
NP_001001552                                                           15    9605
NP_057531                                                              15    9605
NP_001274355                                                           15    9605
NP_001274358                                                           15    9605
NP_003355                                                              15    9605
NP_001274357                                                           15    9605
NP_001037770                                                           15    9605
NP_001185979                                                           15    9605
NP_001185981                                                           15    9605
XP_006721881                                                           15    9605
XP_006721880                                                           15    9605
XP_005249895                                                           15    9605
XP_005249832                                                           15    9605
XP_006715803                                                           15    9605
XP_006715802                                                           15    9605
XP_006715801                                                           15    9605
XP_005248817                                                           15    9605
XP_006715610                                                           15    9605
XP_006711695                                                           15    9605
>NP_005742
          Length = 3907

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RLQAAVEK 9
            RLQAAVEK
Sbjct: 1849 RLQAAVEK 1856
>NP_671714
          Length = 3899

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RLQAAVEK 9
            RLQAAVEK
Sbjct: 1849 RLQAAVEK 1856
>XP_006715890
          Length = 3860

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RLQAAVEK 9
            RLQAAVEK
Sbjct: 1802 RLQAAVEK 1809
>XP_005250160
          Length = 3939

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RLQAAVEK 9
            RLQAAVEK
Sbjct: 1881 RLQAAVEK 1888
>XP_005250159
          Length = 3952

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RLQAAVEK 9
            RLQAAVEK
Sbjct: 1881 RLQAAVEK 1888
>NP_060004
          Length = 1941

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    CRLQAAVEK 9
            C LQAA+E+
Sbjct: 1544 CELQAALEE 1552
>NP_001093582
          Length = 1941

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    CRLQAAVEK 9
            C LQAA+E+
Sbjct: 1544 CELQAALEE 1552
>NP_004865
          Length = 457

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CRLQAAVEK 9
           C++QA +EK
Sbjct: 443 CKIQAILEK 451
>NP_001191807
          Length = 421

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CRLQAAVEK 9
           C++QA +EK
Sbjct: 407 CKIQAILEK 415
>NP_004578
          Length = 977

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CRLQAAVEK 9
           CRLQ  +EK
Sbjct: 890 CRLQEELEK 898
>NP_001036041
          Length = 977

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CRLQAAVEK 9
           CRLQ  +EK
Sbjct: 890 CRLQEELEK 898
>NP_001092903
          Length = 831

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LQAAVEK 9
           LQAAVEK
Sbjct: 758 LQAAVEK 764
>NP_002463
          Length = 1937

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    CRLQAAVEK 9
            C +QAA+E+
Sbjct: 1541 CEIQAALEE 1549
>NP_149022
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CRLQAAVE 8
           C LQAA+E
Sbjct: 195 CSLQAALE 202
>NP_775820
          Length = 374

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LQAAVEK 9
           LQAAVEK
Sbjct: 161 LQAAVEK 167
>XP_005273630
          Length = 205

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  RLQAAVEK 9
          RLQA VEK
Sbjct: 89 RLQAKVEK 96
>NP_061158
          Length = 253

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           RLQA VEK
Sbjct: 137 RLQAKVEK 144
>NP_001157718
          Length = 422

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  CRLQAAVEK 9
          C+L  AVEK
Sbjct: 77 CQLMFAVEK 85
>NP_004378
          Length = 324

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   CRLQAAVE 8
           C LQ AVE
Sbjct: 114 CALQKAVE 121
>NP_001128669
          Length = 594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           RLQ +VEK
Sbjct: 148 RLQTSVEK 155
>NP_055510
          Length = 1427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CRLQAAV 7
           CRL AAV
Sbjct: 918 CRLAAAV 924
>XP_005256934
          Length = 1434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CRLQAAV 7
           CRL AAV
Sbjct: 918 CRLAAAV 924
>XP_006721670
          Length = 1471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CRLQAAV 7
           CRL AAV
Sbjct: 918 CRLAAAV 924
>XP_005265877
          Length = 286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  CRLQAAVE 8
          C LQ AVE
Sbjct: 76 CALQKAVE 83
>XP_005265157
          Length = 577

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           RLQ +VEK
Sbjct: 131 RLQTSVEK 138
>XP_005265156
          Length = 594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           RLQ +VEK
Sbjct: 148 RLQTSVEK 155
>XP_006713187
          Length = 594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           RLQ +VEK
Sbjct: 148 RLQTSVEK 155
>NP_835467
          Length = 594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           RLQ +VEK
Sbjct: 148 RLQTSVEK 155
>NP_060626
          Length = 4363

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    CRLQAAVEK 9
            CRL+  VE+
Sbjct: 2205 CRLKLQVER 2213
>NP_056193
          Length = 4388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    CRLQAAVEK 9
            CRL+  VE+
Sbjct: 2205 CRLKLQVER 2213
>NP_060921
          Length = 1338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   CRLQAAVEK 9
           C+++ AV+K
Sbjct: 672 CKIRKAVQK 680
>NP_066564
          Length = 837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRLQAAVEK 9
           CR  +A+EK
Sbjct: 815 CRCASALEK 823
>NP_001274359
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CRLQAAV 7
           C LQAAV
Sbjct: 124 CGLQAAV 130
>NP_001185980
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  CRLQAAVEK 9
          C+LQ  +EK
Sbjct: 10 CKLQNQLEK 18
>NP_001268369
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LQAAVEK 9
           LQAAV+K
Sbjct: 145 LQAAVDK 151
>NP_001001552
          Length = 108

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  CRLQAAVEK 9
          C+LQ  +EK
Sbjct: 10 CKLQNQLEK 18
>NP_057531
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           +LQ A+EK
Sbjct: 519 KLQTAIEK 526
>NP_001274355
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CRLQAAV 7
           C LQAAV
Sbjct: 261 CGLQAAV 267
>NP_001274358
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CRLQAAV 7
           C LQAAV
Sbjct: 124 CGLQAAV 130
>NP_003355
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CRLQAAV 7
           C LQAAV
Sbjct: 261 CGLQAAV 267
>NP_001274357
          Length = 173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CRLQAAV 7
           C LQAAV
Sbjct: 124 CGLQAAV 130
>NP_001037770
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LQAAVEK 9
           LQAAV+K
Sbjct: 155 LQAAVDK 161
>NP_001185979
          Length = 181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  CRLQAAVEK 9
          C+LQ  +EK
Sbjct: 10 CKLQNQLEK 18
>NP_001185981
          Length = 67

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  CRLQAAVEK 9
          C+LQ  +EK
Sbjct: 10 CKLQNQLEK 18
>XP_006721881
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRLQAAVEK 9
           CR  +A+EK
Sbjct: 455 CRCASALEK 463
>XP_006721880
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRLQAAVEK 9
           CR  +A+EK
Sbjct: 816 CRCASALEK 824
>XP_005249895
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CRLQAAV 7
           C LQAAV
Sbjct: 197 CGLQAAV 203
>XP_005249832
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           +LQ A+EK
Sbjct: 407 KLQTAIEK 414
>XP_006715803
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           +LQ A+EK
Sbjct: 519 KLQTAIEK 526
>XP_006715802
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           +LQ A+EK
Sbjct: 519 KLQTAIEK 526
>XP_006715801
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RLQAAVEK 9
           +LQ A+EK
Sbjct: 519 KLQTAIEK 526
>XP_005248817
          Length = 250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LQAAVEK 9
           LQAAV+K
Sbjct: 155 LQAAVDK 161
>XP_006715610
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LQAAVEK 9
           LQAAV+K
Sbjct: 155 LQAAVDK 161
>XP_006711695
          Length = 181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  CRLQAAVEK 9
          C+LQ  +EK
Sbjct: 10 CKLQNQLEK 18
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.127    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 393,074
Number of extensions: 726
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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