Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     390
NP_001017408                                                           17    4312
NP_065132                                                              17    4312
NP_001006608                                                           16    5631
NP_955372                                                              16    5631
NP_055649                                                              16    5631
XP_006725346                                                           16    5631
XP_005275722                                                           16    5631
XP_005275721                                                           16    5631
XP_006725344                                                           16    5631
XP_006725343                                                           16    5631
XP_006725342                                                           16    5631
XP_005275717                                                           16    5631
XP_005257944                                                           16    5631
XP_005257941                                                           16    5631
XP_005257940                                                           16    5631
XP_005257939                                                           16    5631
XP_006722272                                                           16    5631
XP_005257937                                                           16    5631
XP_005257936                                                           16    5631
XP_006722271                                                           16    5631
NP_004612                                                              16    7355
NP_003296                                                              16    7355
NP_001124170                                                           16    7355
NP_006315                                                              16    7355
XP_006724409                                                           16    7355
XP_005261851                                                           16    7355
XP_005261850                                                           16    7355
XP_006718961                                                           16    7355
XP_006714359                                                           16    7355
XP_006714358                                                           16    7355
NP_001138678                                                           16    7355
NP_004870                                                              15    9605
NP_001277064                                                           15    9605
NP_149094                                                              15    9605
NP_055990                                                              15    9605
NP_001138740                                                           15    9605
NP_065122                                                              15    9605
XP_006725506                                                           15    9605
XP_006715959                                                           15    9605
XP_005265049                                                           15    9605
XP_006713135                                                           15    9605
NP_001135389                                                           15    9605
>NP_004351
          Length = 882

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   CRKAQSVEA 9
           CRKAQ VEA
Sbjct: 695 CRKAQPVEA 703
>NP_001017408
          Length = 454

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  CRKAQSV 7
          C KAQSV
Sbjct: 81 CHKAQSV 87
>NP_065132
          Length = 462

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  CRKAQSV 7
          C KAQSV
Sbjct: 81 CHKAQSV 87
>NP_001006608
          Length = 1700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>NP_955372
          Length = 1634

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>NP_055649
          Length = 1700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_006725346
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CRKAQSVE 8
          C K QS+E
Sbjct: 61 CNKIQSIE 68
>XP_005275722
          Length = 969

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_005275721
          Length = 1018

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_006725344
          Length = 1606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 828 CNKIQSIE 835
>XP_006725343
          Length = 1635

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_006725342
          Length = 1651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_005275717
          Length = 1665

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_005257944
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CRKAQSVE 8
          C K QS+E
Sbjct: 61 CNKIQSIE 68
>XP_005257941
          Length = 969

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_005257940
          Length = 1018

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_005257939
          Length = 1606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 828 CNKIQSIE 835
>XP_006722272
          Length = 1635

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_005257937
          Length = 1651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_005257936
          Length = 1665

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>XP_006722271
          Length = 1700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 922 CNKIQSIE 929
>NP_004612
          Length = 931

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   CRKAQSVEA 9
           CR  + VEA
Sbjct: 336 CRNTEEVEA 344
>NP_003296
          Length = 848

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRKAQSVEA 9
           CR ++ VEA
Sbjct: 277 CRDSEEVEA 285
>NP_001124170
          Length = 921

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRKAQSVEA 9
           CR ++ VEA
Sbjct: 350 CRDSEEVEA 358
>NP_006315
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RKAQSVEA 9
          RKAQS+ A
Sbjct: 53 RKAQSIPA 60
>XP_006724409
          Length = 1806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   CRKAQSVEA 9
           CR   SVEA
Sbjct: 585 CRGGSSVEA 593
>XP_005261851
          Length = 1806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   CRKAQSVEA 9
           CR   SVEA
Sbjct: 585 CRGGSSVEA 593
>XP_005261850
          Length = 1806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   CRKAQSVEA 9
           CR   SVEA
Sbjct: 585 CRGGSSVEA 593
>XP_006718961
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   CRKAQSVEA 9
           CR  + VEA
Sbjct: 336 CRNTEEVEA 344
>XP_006714359
          Length = 544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRKAQSVEA 9
           CR ++ VEA
Sbjct: 277 CRDSEEVEA 285
>XP_006714358
          Length = 820

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRKAQSVEA 9
           CR ++ VEA
Sbjct: 277 CRDSEEVEA 285
>NP_001138678
          Length = 1806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   CRKAQSVEA 9
           CR   SVEA
Sbjct: 585 CRGGSSVEA 593
>NP_004870
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  RKAQSVE 8
          R+AQS+E
Sbjct: 52 RRAQSIE 58
>NP_001277064
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  RKAQSVE 8
          R+AQS+E
Sbjct: 38 RRAQSIE 44
>NP_149094
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   CRKAQSVE 8
           C+K +S+E
Sbjct: 430 CKKLRSIE 437
>NP_055990
          Length = 2754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  CRKAQSVEA 9
          CR  +++EA
Sbjct: 89 CRNLENIEA 97
>NP_001138740
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 161 CVKVQSIE 168
>NP_065122
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   CRKAQSVEA 9
           CR  + VEA
Sbjct: 281 CRDTEEVEA 289
>XP_006725506
          Length = 250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRKAQSVEA 9
           CR +QSV +
Sbjct: 141 CRASQSVSS 149
>XP_006715959
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C K QS+E
Sbjct: 161 CVKVQSIE 168
>XP_005265049
          Length = 2720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  CRKAQSVEA 9
          CR  +++EA
Sbjct: 82 CRNLENIEA 90
>XP_006713135
          Length = 2727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  CRKAQSVEA 9
          CR  +++EA
Sbjct: 89 CRNLENIEA 97
>NP_001135389
          Length = 1335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   CRKAQSVE 8
           C KAQS E
Sbjct: 556 CGKAQSPE 563
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.114    0.318 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 319,640
Number of extensions: 286
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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