Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     299
XP_006713721                                                           20     299
NP_056161                                                              17    3301
XP_006720156                                                           17    3301
XP_006712935                                                           16    5631
XP_006712934                                                           16    5631
XP_006712933                                                           16    5631
NP_852118                                                              16    5631
NP_001026                                                              16    7355
NP_064551                                                              16    7355
NP_001159687                                                           16    7355
NP_110387                                                              16    7355
XP_005259994                                                           16    7355
XP_006711873                                                           16    7355
XP_006711871                                                           16    7355
XP_006711870                                                           16    7355
XP_006711869                                                           16    7355
XP_006711868                                                           16    7355
XP_006711867                                                           16    7355
XP_006711866                                                           16    7355
XP_006711865                                                           16    7355
NP_000408                                                              15    9605
NP_066919                                                              15    9605
NP_001003406                                                           15    9605
XP_005252503                                                           15    9605
NP_004465                                                              15    9605
>NP_006209
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   CQIMSSAKR 9
           C+IMSSAKR
Sbjct: 769 CRIMSSAKR 777
>XP_006713721
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   CQIMSSAKR 9
           C+IMSSAKR
Sbjct: 769 CRIMSSAKR 777
>NP_056161
          Length = 2539

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CQIMSSAKR 9
           CQ + SAKR
Sbjct: 377 CQSLESAKR 385
>XP_006720156
          Length = 2497

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CQIMSSAKR 9
           CQ + SAKR
Sbjct: 377 CQSLESAKR 385
>XP_006712935
          Length = 977

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CQIMSSAK 8
           CQI +SAK
Sbjct: 260 CQIEASAK 267
>XP_006712934
          Length = 1583

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CQIMSSAK 8
           CQI +SAK
Sbjct: 156 CQIEASAK 163
>XP_006712933
          Length = 1687

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CQIMSSAK 8
           CQI +SAK
Sbjct: 260 CQIEASAK 267
>NP_852118
          Length = 1684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CQIMSSAK 8
           CQI +SAK
Sbjct: 257 CQIEASAK 264
>NP_001026
          Length = 4967

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    QIMSSAKR 9
            +IM SAKR
Sbjct: 4137 EIMGSAKR 4144
>NP_064551
          Length = 725

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  CQIMSSAKR 9
          C++M +AK+
Sbjct: 62 CRVMRTAKK 70
>NP_001159687
          Length = 398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   CQIMSSAKR 9
           CQ+ ++A+R
Sbjct: 217 CQVRANARR 225
>NP_110387
          Length = 398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   CQIMSSAKR 9
           CQ+ ++A+R
Sbjct: 217 CQVRANARR 225
>XP_005259994
          Length = 500

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   CQIMSSAKR 9
           CQ+ ++A+R
Sbjct: 319 CQVRANARR 327
>XP_006711873
          Length = 4954

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    QIMSSAKR 9
            +IM SAKR
Sbjct: 4124 EIMGSAKR 4131
>XP_006711871
          Length = 4906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    QIMSSAKR 9
            +IM SAKR
Sbjct: 4076 EIMGSAKR 4083
>XP_006711870
          Length = 4965

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    QIMSSAKR 9
            +IM SAKR
Sbjct: 4135 EIMGSAKR 4142
>XP_006711869
          Length = 4973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    QIMSSAKR 9
            +IM SAKR
Sbjct: 4143 EIMGSAKR 4150
>XP_006711868
          Length = 4975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    QIMSSAKR 9
            +IM SAKR
Sbjct: 4145 EIMGSAKR 4152
>XP_006711867
          Length = 4977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    QIMSSAKR 9
            +IM SAKR
Sbjct: 4147 EIMGSAKR 4154
>XP_006711866
          Length = 4984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    QIMSSAKR 9
            +IM SAKR
Sbjct: 4154 EIMGSAKR 4161
>XP_006711865
          Length = 4985

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    QIMSSAKR 9
            +IM SAKR
Sbjct: 4155 EIMGSAKR 4162
>NP_000408
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CQIMSSAKR 9
          CQ  SSA R
Sbjct: 80 CQCTSSATR 88
>NP_066919
          Length = 2223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   CQIMSSAKR 9
           C I+  AKR
Sbjct: 443 CHILRKAKR 451
>NP_001003406
          Length = 2188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   CQIMSSAKR 9
           C I+  AKR
Sbjct: 443 CHILRKAKR 451
>XP_005252503
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CQIMSSAKR 9
          CQ  SSA R
Sbjct: 80 CQCTSSATR 88
>NP_004465
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  CQIMSS 6
          C+IMSS
Sbjct: 8  CEIMSS 13
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.118    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 288,539
Number of extensions: 227
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package