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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005202                                                              19     665
NP_000213                                                              19     665
NP_001087241                                                           19     665
NP_001275634                                                           19     665
XP_005265799                                                           19     665
XP_005265798                                                           19     665
XP_005265797                                                           19     665
NP_006197                                                              17    2528
NP_002600                                                              17    2528
XP_005268521                                                           17    2528
XP_005265800                                                           17    2528
XP_006714103                                                           17    2528
XP_006714102                                                           17    2528
NP_001007526                                                           16    5631
NP_001277284                                                           16    5631
NP_001019554                                                           16    7355
NP_001445                                                              16    7355
NP_775490                                                              16    7355
NP_055718                                                              16    7355
NP_002855                                                              16    7355
XP_006726866                                                           16    7355
XP_006723983                                                           16    7355
XP_006719739                                                           16    7355
XP_006719738                                                           16    7355
XP_005269272                                                           16    7355
XP_006717373                                                           16    7355
XP_005265662                                                           16    7355
XP_006710157                                                           16    7355
NP_001239                                                              16    7355
NP_001726                                                              15    9605
NP_073751                                                              15    9605
NP_005012                                                              15    9605
NP_808211                                                              15    9605
NP_803545                                                              15    9605
NP_003703                                                              15    9605
XP_005258189                                                           15    9605
XP_006722402                                                           15    9605

>NP_005202
          Length = 972

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAFLASKN 9
           MAFLASKN
Sbjct: 766 MAFLASKN 773



>NP_000213
          Length = 976

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAFLASKN 9
           MAFLASKN
Sbjct: 780 MAFLASKN 787



>NP_001087241
          Length = 972

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAFLASKN 9
           MAFLASKN
Sbjct: 776 MAFLASKN 783



>NP_001275634
          Length = 972

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAFLASKN 9
           MAFLASKN
Sbjct: 766 MAFLASKN 773



>XP_005265799
          Length = 973

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAFLASKN 9
           MAFLASKN
Sbjct: 777 MAFLASKN 784



>XP_005265798
          Length = 976

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAFLASKN 9
           MAFLASKN
Sbjct: 780 MAFLASKN 787



>XP_005265797
          Length = 977

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   MAFLASKN 9
           MAFLASKN
Sbjct: 781 MAFLASKN 788



>NP_006197
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MAFLASKN 9
           M FLASKN
Sbjct: 806 MEFLASKN 813



>NP_002600
          Length = 1106

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MAFLASKN 9
           M FLASKN
Sbjct: 814 MEFLASKN 821



>XP_005268521
          Length = 1042

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MAFLASKN 9
           M FLASKN
Sbjct: 750 MEFLASKN 757



>XP_005265800
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MAFLASKN 9
           M FLASKN
Sbjct: 806 MEFLASKN 813



>XP_006714103
          Length = 1111

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MAFLASKN 9
           M FLASKN
Sbjct: 828 MEFLASKN 835



>XP_006714102
          Length = 1114

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MAFLASKN 9
           M FLASKN
Sbjct: 831 MEFLASKN 838



>NP_001007526
          Length = 1432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    CMAFLASK 8
            CMA LAS+
Sbjct: 1005 CMAVLASQ 1012



>NP_001277284
          Length = 1429

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CMAFLASK 8
           CMA LAS+
Sbjct: 870 CMAVLASQ 877



>NP_001019554
          Length = 2385

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   CMAFLASK 8
           C++FLA++
Sbjct: 145 CLSFLAAR 152



>NP_001445
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   CMAFLASK 8
           CMA  ASK
Sbjct: 131 CMAMQASK 138



>NP_775490
          Length = 783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CMAFLASKN 9
           C+ F ASK+
Sbjct: 508 CLTFSASKS 516



>NP_055718
          Length = 2363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   CMAFLASK 8
           C++FLA++
Sbjct: 145 CLSFLAAR 152



>NP_002855
          Length = 1482

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AFLASKN 9
           AFLAS+N
Sbjct: 839 AFLASQN 845



>XP_006726866
          Length = 783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CMAFLASKN 9
           C+ F ASK+
Sbjct: 508 CLTFSASKS 516



>XP_006723983
          Length = 783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CMAFLASKN 9
           C+ F ASK+
Sbjct: 508 CLTFSASKS 516



>XP_006719739
          Length = 2306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  CMAFLASK 8
          C++FLA++
Sbjct: 88 CLSFLAAR 95



>XP_006719738
          Length = 2340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   CMAFLASK 8
           C++FLA++
Sbjct: 145 CLSFLAAR 152



>XP_005269272
          Length = 2356

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   CMAFLASK 8
           C++FLA++
Sbjct: 145 CLSFLAAR 152



>XP_006717373
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  CMAFLA 6
          C+AFLA
Sbjct: 6  CLAFLA 11



>XP_005265662
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   CMAFLA 6
           C+AFLA
Sbjct: 501 CLAFLA 506



>XP_006710157
          Length = 783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CMAFLASKN 9
           C+ F ASK+
Sbjct: 508 CLTFSASKS 516



>NP_001239
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   CMAFLA 6
           C+AFLA
Sbjct: 501 CLAFLA 506



>NP_001726
          Length = 1676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    CMAFLAS 7
            C AFLA+
Sbjct: 1657 CQAFLAN 1663



>NP_073751
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  CMAFLAS 7
          C AF+AS
Sbjct: 59 CAAFIAS 65



>NP_005012
          Length = 875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   CMAFLASKN 9
           C  +LA KN
Sbjct: 679 CSFYLADKN 687



>NP_808211
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   CMAFLA 6
           CM FLA
Sbjct: 196 CMVFLA 201



>NP_803545
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   CMAFLA 6
           CM FLA
Sbjct: 119 CMVFLA 124



>NP_003703
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   CMAFLA 6
           CM FLA
Sbjct: 175 CMVFLA 180



>XP_005258189
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  CMAFLAS 7
          C AF+AS
Sbjct: 59 CAAFIAS 65



>XP_006722402
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  CMAFLAS 7
          C AF+AS
Sbjct: 59 CAAFIAS 65



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.126    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 356,514
Number of extensions: 635
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)