Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001274741                                                           18    1482
NP_071765                                                              17    4312
NP_853517                                                              17    4312
XP_006721927                                                           17    4312
XP_005257364                                                           17    4312
NP_057675                                                              16    5631
NP_115992                                                              16    5631
NP_001276093                                                           16    5631
NP_001276094                                                           16    5631
NP_998768                                                              16    5631
XP_005250338                                                           16    5631
NP_001032242                                                           16    7355
NP_001229813                                                           16    7355
NP_056369                                                              16    7355
NP_001274533                                                           16    7355
NP_001274532                                                           16    7355
NP_542782                                                              16    7355
NP_003434                                                              16    7355
XP_005261690                                                           16    7355
XP_005254355                                                           16    7355
XP_005246044                                                           16    7355
XP_005246043                                                           16    7355
NP_005484                                                              15    9605
NP_060290                                                              15    9605
XP_006720925                                                           15    9605
XP_006720924                                                           15    9605
XP_006720923                                                           15    9605
XP_005255218                                                           15    9605
XP_005255217                                                           15    9605
XP_006717224                                                           15    9605
XP_006717223                                                           15    9605
XP_006717222                                                           15    9605
XP_006717221                                                           15    9605
XP_006717220                                                           15    9605
XP_006717219                                                           15    9605
XP_006717218                                                           15    9605
XP_006717217                                                           15    9605
XP_006717216                                                           15    9605
XP_006717215                                                           15    9605
XP_006717214                                                           15    9605
XP_006715992                                                           15    9605
XP_006715008                                                           15    9605
>NP_001274741
          Length = 131

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  CLGSEARS 8
          CLGS ARS
Sbjct: 48 CLGSAARS 55
>NP_071765
          Length = 1386

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   CLGSEAR 7
           CLG+E+R
Sbjct: 810 CLGNESR 816
>NP_853517
          Length = 468

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  CLGSEARS 8
          C+GS ARS
Sbjct: 34 CVGSGARS 41
>XP_006721927
          Length = 465

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  CLGSEARS 8
          C+GS ARS
Sbjct: 34 CVGSGARS 41
>XP_005257364
          Length = 466

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  CLGSEARS 8
          C+GS ARS
Sbjct: 34 CVGSGARS 41
>NP_057675
          Length = 453

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   CLGSEARSV 9
           C GS  RSV
Sbjct: 382 CTGSHLRSV 390
>NP_115992
          Length = 753

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   CLGSEARS 8
           CL S ARS
Sbjct: 560 CLASSARS 567
>NP_001276093
          Length = 608

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   CLGSEARS 8
           CL S ARS
Sbjct: 415 CLASSARS 422
>NP_001276094
          Length = 392

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   CLGSEARS 8
           CL S ARS
Sbjct: 199 CLASSARS 206
>NP_998768
          Length = 425

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  CLGSEAR 7
          C GSEAR
Sbjct: 87 CPGSEAR 93
>XP_005250338
          Length = 418

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  CLGSEAR 7
          C GSEAR
Sbjct: 80 CPGSEAR 86
>NP_001032242
          Length = 733

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGSEARSV 9
           LGSEAR +
Sbjct: 560 LGSEARGL 567
>NP_001229813
          Length = 721

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGSEARSV 9
           LGSEAR +
Sbjct: 198 LGSEARGL 205
>NP_056369
          Length = 617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGSEARSV 9
           LG EARS+
Sbjct: 530 LGKEARSL 537
>NP_001274533
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGSEARSV 9
           LGSEAR +
Sbjct: 204 LGSEARGL 211
>NP_001274532
          Length = 810

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGSEARSV 9
           LGSEAR +
Sbjct: 280 LGSEARGL 287
>NP_542782
          Length = 575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           C G+EAR
Sbjct: 413 CAGTEAR 419
>NP_003434
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGSEARSV 9
           LGSEAR +
Sbjct: 280 LGSEARGL 287
>XP_005261690
          Length = 316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  GSEARSV 9
          GSEARS+
Sbjct: 13 GSEARSL 19
>XP_005254355
          Length = 617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGSEARSV 9
           LG EARS+
Sbjct: 530 LGKEARSL 537
>XP_005246044
          Length = 734

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGSEARSV 9
           LGSEAR +
Sbjct: 204 LGSEARGL 211
>XP_005246043
          Length = 747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LGSEARSV 9
           LGSEAR +
Sbjct: 217 LGSEARGL 224
>NP_005484
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   CLGSEARS 8
           CLG  ARS
Sbjct: 707 CLGLMARS 714
>NP_060290
          Length = 876

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 647 CLGVDAR 653
>XP_006720925
          Length = 1674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    CLGSEARS 8
            C GS ARS
Sbjct: 1377 CKGSGARS 1384
>XP_006720924
          Length = 1679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    CLGSEARS 8
            C GS ARS
Sbjct: 1305 CKGSGARS 1312
>XP_006720923
          Length = 1738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    CLGSEARS 8
            C GS ARS
Sbjct: 1364 CKGSGARS 1371
>XP_005255218
          Length = 1750

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    CLGSEARS 8
            C GS ARS
Sbjct: 1376 CKGSGARS 1383
>XP_005255217
          Length = 1751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    CLGSEARS 8
            C GS ARS
Sbjct: 1377 CKGSGARS 1384
>XP_006717224
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 699 CLGVDAR 705
>XP_006717223
          Length = 557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 328 CLGVDAR 334
>XP_006717222
          Length = 557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 328 CLGVDAR 334
>XP_006717221
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 700 CLGVDAR 706
>XP_006717220
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 700 CLGVDAR 706
>XP_006717219
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 700 CLGVDAR 706
>XP_006717218
          Length = 877

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 648 CLGVDAR 654
>XP_006717217
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 676 CLGVDAR 682
>XP_006717216
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 700 CLGVDAR 706
>XP_006717215
          Length = 928

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 699 CLGVDAR 705
>XP_006717214
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLGSEAR 7
           CLG +AR
Sbjct: 700 CLGVDAR 706
>XP_006715992
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  LGSEAR 7
          LGSEAR
Sbjct: 50 LGSEAR 55
>XP_006715008
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   CLGSEARS 8
           CLG  ARS
Sbjct: 478 CLGLMARS 485
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.126    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 464,004
Number of extensions: 471
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package