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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              19     665
XP_005266703                                                           19     665
XP_006726780                                                           17    4312
XP_006721690                                                           17    4312
XP_006710091                                                           17    4312
NP_073625                                                              16    5631
NP_795345                                                              16    5631
NP_116202                                                              16    5631
NP_057330                                                              16    5631
NP_001073137                                                           16    5631
NP_001273382                                                           16    5631
XP_005259280                                                           16    5631
XP_005259279                                                           16    5631
XP_005254137                                                           16    5631
XP_006720017                                                           16    5631
NP_037494                                                              16    5631
NP_001153797                                                           16    5631
NP_997186                                                              16    7355
NP_150375                                                              16    7355
NP_653167                                                              16    7355
NP_115960                                                              16    7355
NP_001276087                                                           16    7355
NP_001258777                                                           16    7355
XP_005261634                                                           16    7355
NP_001276088                                                           16    7355
NP_001035915                                                           15    9605
NP_689564                                                              15    9605
NP_001257578                                                           15    9605
NP_001257577                                                           15    9605
NP_002842                                                              15    9605
NP_001193767                                                           15    9605
XP_006721514                                                           15    9605
XP_006721513                                                           15    9605
XP_005256514                                                           15    9605
XP_006716139                                                           15    9605
XP_005250576                                                           15    9605
NP_001193768                                                           15    9605

>NP_443179
          Length = 2170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    KAFRSTVM 9
            KAFRSTVM
Sbjct: 1932 KAFRSTVM 1939



>XP_005266703
          Length = 1501

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    KAFRSTVM 9
            KAFRSTVM
Sbjct: 1263 KAFRSTVM 1270



>XP_006726780
          Length = 293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CKAFRSTV 8
           C++FRS V
Sbjct: 107 CRSFRSVV 114



>XP_006721690
          Length = 293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CKAFRSTV 8
           C++FRS V
Sbjct: 107 CRSFRSVV 114



>XP_006710091
          Length = 293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CKAFRSTV 8
           C++FRS V
Sbjct: 107 CRSFRSVV 114



>NP_073625
          Length = 342

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   CKAFRSTVM 9
           CK+FR++++
Sbjct: 302 CKSFRNSLI 310



>NP_795345
          Length = 342

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   CKAFRSTVM 9
           CK+FR++++
Sbjct: 302 CKSFRNSLI 310



>NP_116202
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CKAFRST 7
           CKAFR +
Sbjct: 208 CKAFRES 214



>NP_057330
          Length = 270

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CKAFRST 7
           CKA RST
Sbjct: 255 CKASRST 261



>NP_001073137
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CKAFRST 7
           CKAFR +
Sbjct: 189 CKAFRES 195



>NP_001273382
          Length = 630

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  CKAFRST 7
          CKAFR +
Sbjct: 78 CKAFRES 84



>XP_005259280
          Length = 623

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   CKAFRS 6
           CK+FRS
Sbjct: 211 CKSFRS 216



>XP_005259279
          Length = 706

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   CKAFRS 6
           CK+FRS
Sbjct: 294 CKSFRS 299



>XP_005254137
          Length = 632

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CKAFRST 7
           CKAFR +
Sbjct: 208 CKAFRES 214



>XP_006720017
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CKAFRST 7
           CKAFR +
Sbjct: 189 CKAFRES 195



>NP_037494
          Length = 706

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   CKAFRS 6
           CK+FRS
Sbjct: 294 CKSFRS 299



>NP_001153797
          Length = 317

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KAFRSTVM 9
           +AFR TVM
Sbjct: 299 EAFRKTVM 306



>NP_997186
          Length = 1379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   CKAFR 5
           CKAFR
Sbjct: 615 CKAFR 619



>NP_150375
          Length = 1196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   CKAFR 5
           CKAFR
Sbjct: 743 CKAFR 747



>NP_653167
          Length = 742

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KAFRST 7
           KAFRST
Sbjct: 453 KAFRST 458



>NP_115960
          Length = 652

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CKAFRST 7
           CKA R+T
Sbjct: 381 CKAIRNT 387



>NP_001276087
          Length = 600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CKAFRST 7
           CKA R+T
Sbjct: 329 CKAIRNT 335



>NP_001258777
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KAFRST 7
           KAFRST
Sbjct: 456 KAFRST 461



>XP_005261634
          Length = 632

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   CKAFRS 6
           CK FRS
Sbjct: 614 CKTFRS 619



>NP_001276088
          Length = 526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CKAFRST 7
           CKA R+T
Sbjct: 255 CKAIRNT 261



>NP_001035915
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  CKAFRSTV 8
          C+A R+TV
Sbjct: 40 CRASRNTV 47



>NP_689564
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  CKAFRSTV 8
          C+A R+TV
Sbjct: 40 CRASRNTV 47



>NP_001257578
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  CKAFRSTV 8
          C+A R+TV
Sbjct: 40 CRASRNTV 47



>NP_001257577
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  CKAFRSTV 8
          C+A R+TV
Sbjct: 40 CRASRNTV 47



>NP_002842
          Length = 2315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    CKAFRSTVM 9
            C++F+ T+M
Sbjct: 2144 CESFKVTLM 2152



>NP_001193767
          Length = 1455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    CKAFRSTVM 9
            C++F+ T+M
Sbjct: 1284 CESFKVTLM 1292



>XP_006721514
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  CKAFRSTV 8
          C+A R+TV
Sbjct: 40 CRASRNTV 47



>XP_006721513
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  CKAFRSTV 8
          C+A R+TV
Sbjct: 40 CRASRNTV 47



>XP_005256514
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  CKAFRSTV 8
          C+A R+TV
Sbjct: 40 CRASRNTV 47



>XP_006716139
          Length = 1869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    CKAFRSTVM 9
            C++F+ T+M
Sbjct: 1698 CESFKVTLM 1706



>XP_005250576
          Length = 2308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    CKAFRSTVM 9
            C++F+ T+M
Sbjct: 2137 CESFKVTLM 2145



>NP_001193768
          Length = 1448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    CKAFRSTVM 9
            C++F+ T+M
Sbjct: 1277 CESFKVTLM 1285



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.341    0.129    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 410,069
Number of extensions: 625
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)