Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001093112                                                           17    3301
NP_060818                                                              17    3301
XP_005256104                                                           17    3301
XP_005256103                                                           17    3301
NP_072179                                                              17    4312
NP_002881                                                              17    4312
NP_114432                                                              17    4312
NP_001269609                                                           17    4312
NP_001269608                                                           17    4312
NP_689804                                                              17    4312
NP_001273173                                                           17    4312
NP_001273172                                                           17    4312
XP_006719326                                                           17    4312
XP_006719325                                                           17    4312
XP_005251961                                                           17    4312
XP_006717106                                                           17    4312
XP_006717105                                                           17    4312
XP_005251958                                                           17    4312
NP_000292                                                              16    5631
NP_002514                                                              16    5631
NP_003250                                                              16    5631
NP_003900                                                              16    7355
NP_001186363                                                           16    7355
NP_775862                                                              16    7355
XP_005258234                                                           16    7355
XP_005268859                                                           16    7355
XP_005251150                                                           16    7355
NP_057185                                                              16    7355
NP_001167639                                                           15    9605
NP_001167638                                                           15    9605
NP_001128524                                                           15    9605
NP_003168                                                              15    9605
NP_002342                                                              15    9605
NP_001108409                                                           15    9605
NP_001269796                                                           15    9605
NP_001269800                                                           15    9605
NP_001269801                                                           15    9605
NP_001269799                                                           15    9605
NP_001121163                                                           15    9605
NP_057208                                                              15    9605
NP_001269797                                                           15    9605
NP_001269798                                                           15    9605
XP_005252204                                                           15    9605
NP_899234                                                              15    9605
NP_066939                                                              15    9605
>NP_001093112
          Length = 770

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CHGKVSKTL 9
           C+G++ KTL
Sbjct: 511 CNGEILKTL 519
>NP_060818
          Length = 770

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CHGKVSKTL 9
           C+G++ KTL
Sbjct: 511 CNGEILKTL 519
>XP_005256104
          Length = 770

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CHGKVSKTL 9
           C+G++ KTL
Sbjct: 511 CNGEILKTL 519
>XP_005256103
          Length = 770

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CHGKVSKTL 9
           C+G++ KTL
Sbjct: 511 CNGEILKTL 519
>NP_072179
          Length = 870

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HGKVSK 7
           HGK+SK
Sbjct: 176 HGKISK 181
>NP_002881
          Length = 1047

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HGKVSK 7
           HGK+SK
Sbjct: 353 HGKISK 358
>NP_114432
          Length = 1249

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CHGKVSKTL 9
           C GKVS+ L
Sbjct: 761 CRGKVSEGL 769
>NP_001269609
          Length = 1460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HGKVSKTL 9
           HGK  KTL
Sbjct: 556 HGKPDKTL 563
>NP_001269608
          Length = 1478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HGKVSKTL 9
           HGK  KTL
Sbjct: 556 HGKPDKTL 563
>NP_689804
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   HGKVSKTL 9
           +G+VSKTL
Sbjct: 400 YGEVSKTL 407
>NP_001273173
          Length = 484

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   HGKVSKTL 9
           +G+VSKTL
Sbjct: 321 YGEVSKTL 328
>NP_001273172
          Length = 523

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   HGKVSKTL 9
           +G+VSKTL
Sbjct: 360 YGEVSKTL 367
>XP_006719326
          Length = 539

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   HGKVSKTL 9
           +G+VSKTL
Sbjct: 400 YGEVSKTL 407
>XP_006719325
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   HGKVSKTL 9
           +G+VSKTL
Sbjct: 400 YGEVSKTL 407
>XP_005251961
          Length = 1433

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HGKVSKTL 9
           HGK  KTL
Sbjct: 556 HGKPDKTL 563
>XP_006717106
          Length = 1460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HGKVSKTL 9
           HGK  KTL
Sbjct: 556 HGKPDKTL 563
>XP_006717105
          Length = 1460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HGKVSKTL 9
           HGK  KTL
Sbjct: 556 HGKPDKTL 563
>XP_005251958
          Length = 1460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HGKVSKTL 9
           HGK  KTL
Sbjct: 556 HGKPDKTL 563
>NP_000292
          Length = 810

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GKVSKTL 9
           GK+SKT+
Sbjct: 195 GKISKTM 201
>NP_002514
          Length = 431

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HGKVSKTL 9
           +GK+ KTL
Sbjct: 237 YGKIKKTL 244
>NP_003250
          Length = 924

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HGKVSKTL 9
           HG V+KT+
Sbjct: 651 HGSVAKTV 658
>NP_003900
          Length = 537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   CHGKVSKTL 9
           C+G + KT+
Sbjct: 202 CYGDMDKTI 210
>NP_001186363
          Length = 133

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CHGKVSKT 8
          C GK +KT
Sbjct: 69 CFGKFTKT 76
>NP_775862
          Length = 2344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    CHGKVS 6
            CH KVS
Sbjct: 1743 CHDKVS 1748
>XP_005258234
          Length = 933

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CHGKVSKT 8
          C G +SKT
Sbjct: 14 CKGSLSKT 21
>XP_005268859
          Length = 2361

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    CHGKVS 6
            CH KVS
Sbjct: 1760 CHDKVS 1765
>XP_005251150
          Length = 537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   CHGKVSKTL 9
           C+G + KT+
Sbjct: 202 CYGDMDKTI 210
>NP_057185
          Length = 180

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  CHGKVSKT 8
          C GK +KT
Sbjct: 69 CFGKFTKT 76
>NP_001167639
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   HGKVSK 7
           HGK+S+
Sbjct: 170 HGKISR 175
>NP_001167638
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   HGKVSK 7
           HGK+S+
Sbjct: 170 HGKISR 175
>NP_001128524
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   HGKVSK 7
           HGK+S+
Sbjct: 170 HGKISR 175
>NP_003168
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   HGKVSK 7
           HGK+S+
Sbjct: 170 HGKISR 175
>NP_002342
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2  HGKVSK 7
          HGK+S+
Sbjct: 8  HGKISR 13
>NP_001108409
          Length = 125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2  HGKVSK 7
          HGK+S+
Sbjct: 8  HGKISR 13
>NP_001269796
          Length = 251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GKVSKT 8
           GK+SKT
Sbjct: 97  GKISKT 102
>NP_001269800
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GKVSKT 8
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001269801
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GKVSKT 8
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001269799
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GKVSKT 8
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001121163
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GKVSKT 8
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_057208
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GKVSKT 8
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001269797
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GKVSKT 8
           GK+SKT
Sbjct: 263 GKISKT 268
>NP_001269798
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GKVSKT 8
           GK+SKT
Sbjct: 263 GKISKT 268
>XP_005252204
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   HGKVSK 7
           HGK+S+
Sbjct: 170 HGKISR 175
>NP_899234
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GKVSKT 8
           GK+SKT
Sbjct: 179 GKISKT 184
>NP_066939
          Length = 1119

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    CHGKVS 6
            C GKVS
Sbjct: 1037 CRGKVS 1042
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.131    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 546,222
Number of extensions: 756
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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