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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           21     175
NP_001257437                                                           21     175
NP_006281                                                              21     175
XP_006715618                                                           21     175
XP_005267176                                                           21     175
NP_055772                                                              17    3301
XP_005250902                                                           17    3301
XP_005250901                                                           17    3301
XP_005250900                                                           17    3301
XP_005250899                                                           17    3301
XP_005250898                                                           17    3301
XP_005250897                                                           17    3301
XP_005250896                                                           17    3301
XP_005250895                                                           17    3301
NP_001017979                                                           17    4312
NP_001153073                                                           17    4312
NP_004240                                                              17    4312
XP_005248274                                                           17    4312
XP_005248272                                                           17    4312
NP_065761                                                              16    7355
NP_005545                                                              15    9605
NP_005546                                                              15    9605
NP_775109                                                              15    9605
NP_001554                                                              15    9605
NP_001269297                                                           15    9605
NP_000414                                                              15    9605
NP_258259                                                              15    9605
NP_056932                                                              15    9605
NP_004684                                                              15    9605
NP_476429                                                              15    9605
NP_002263                                                              15    9605
XP_006719456                                                           15    9605
XP_005268916                                                           15    9605
XP_006719455                                                           15    9605

>NP_001257436
          Length = 790

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   CFKRTAAEA 9
           CFKRT AEA
Sbjct: 539 CFKRTTAEA 547



>NP_001257437
          Length = 790

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   CFKRTAAEA 9
           CFKRT AEA
Sbjct: 539 CFKRTTAEA 547



>NP_006281
          Length = 790

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   CFKRTAAEA 9
           CFKRT AEA
Sbjct: 539 CFKRTTAEA 547



>XP_006715618
          Length = 577

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   CFKRTAAEA 9
           CFKRT AEA
Sbjct: 326 CFKRTTAEA 334



>XP_005267176
          Length = 790

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   CFKRTAAEA 9
           CFKRT AEA
Sbjct: 539 CFKRTTAEA 547



>NP_055772
          Length = 1198

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CFKRTAAEA 9
           C+K T AEA
Sbjct: 695 CWKETEAEA 703



>XP_005250902
          Length = 808

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CFKRTAAEA 9
           C+K T AEA
Sbjct: 287 CWKETEAEA 295



>XP_005250901
          Length = 900

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CFKRTAAEA 9
           C+K T AEA
Sbjct: 379 CWKETEAEA 387



>XP_005250900
          Length = 1048

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CFKRTAAEA 9
           C+K T AEA
Sbjct: 527 CWKETEAEA 535



>XP_005250899
          Length = 1218

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CFKRTAAEA 9
           C+K T AEA
Sbjct: 697 CWKETEAEA 705



>XP_005250898
          Length = 1226

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CFKRTAAEA 9
           C+K T AEA
Sbjct: 723 CWKETEAEA 731



>XP_005250897
          Length = 1244

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CFKRTAAEA 9
           C+K T AEA
Sbjct: 723 CWKETEAEA 731



>XP_005250896
          Length = 1278

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CFKRTAAEA 9
           C+K T AEA
Sbjct: 775 CWKETEAEA 783



>XP_005250895
          Length = 1296

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CFKRTAAEA 9
           C+K T AEA
Sbjct: 775 CWKETEAEA 783



>NP_001017979
          Length = 221

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CFKRTAAE 8
           CF++ AAE
Sbjct: 169 CFQKVAAE 176



>NP_001153073
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CFKRTAAE 8
           CF++ AAE
Sbjct: 169 CFQKVAAE 176



>NP_004240
          Length = 220

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CFKRTAAE 8
           CF++ AAE
Sbjct: 169 CFQKVAAE 176



>XP_005248274
          Length = 194

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CFKRTAAE 8
           CF++ AAE
Sbjct: 169 CFQKVAAE 176



>XP_005248272
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CFKRTAAE 8
           CF++ AAE
Sbjct: 169 CFQKVAAE 176



>NP_065761
          Length = 583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   CFKRTAA 7
           C KRTAA
Sbjct: 367 CEKRTAA 373



>NP_005545
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 259 KRTAAE 264



>NP_005546
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 259 KRTAAE 264



>NP_775109
          Length = 564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 259 KRTAAE 264



>NP_001554
          Length = 797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FKRTAAEA 9
           FKR +AEA
Sbjct: 308 FKRHSAEA 315



>NP_001269297
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FKRTAAEA 9
           FKR +AEA
Sbjct: 230 FKRHSAEA 237



>NP_000414
          Length = 639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 274 KRTAAE 279



>NP_258259
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 226 KRTAAE 231



>NP_056932
          Length = 638

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 279 KRTAAE 284



>NP_004684
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 245 KRTAAE 250



>NP_476429
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 296 KRTAAE 301



>NP_002263
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 233 KRTAAE 238



>XP_006719456
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 248 KRTAAE 253



>XP_005268916
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 274 KRTAAE 279



>XP_006719455
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KRTAAE 8
           KRTAAE
Sbjct: 284 KRTAAE 289



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.123    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 336,886
Number of extensions: 267
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)