Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           17    2528
NP_000240                                                              17    2528
NP_001161089                                                           17    2528
XP_005265218                                                           17    2528
NP_003137                                                              17    4312
XP_005274251                                                           17    4312
NP_002212                                                              16    5631
NP_056153                                                              16    5631
XP_006723795                                                           16    5631
XP_006723794                                                           16    5631
XP_006723793                                                           16    5631
XP_006723792                                                           16    5631
XP_006723791                                                           16    5631
XP_006723790                                                           16    5631
XP_005273177                                                           16    5631
NP_001265577                                                           16    7355
NP_001139811                                                           16    7355
NP_775929                                                              16    7355
NP_001265576                                                           16    7355
NP_005332                                                              16    7355
XP_005265147                                                           16    7355
XP_006713180                                                           16    7355
XP_006710655                                                           16    7355
NP_057510                                                              15    9605
XP_005261684                                                           15    9605
NP_005006                                                              15    9605
>NP_001245200
          Length = 687

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   CAYRAS 6
           CAYRAS
Sbjct: 124 CAYRAS 129
>NP_000240
          Length = 756

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   CAYRAS 6
           CAYRAS
Sbjct: 124 CAYRAS 129
>NP_001161089
          Length = 658

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  CAYRAS 6
          CAYRAS
Sbjct: 26 CAYRAS 31
>XP_005265218
          Length = 687

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   CAYRAS 6
           CAYRAS
Sbjct: 124 CAYRAS 129
>NP_003137
          Length = 709

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   CAYRASS 7
           C+Y+ASS
Sbjct: 343 CSYKASS 349
>XP_005274251
          Length = 712

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   CAYRASS 7
           C+Y+ASS
Sbjct: 343 CSYKASS 349
>NP_002212
          Length = 946

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CAYRASSQ 8
           C +R+SSQ
Sbjct: 178 CPFRSSSQ 185
>NP_056153
          Length = 1541

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AYRASSQ 8
           +YRASSQ
Sbjct: 151 SYRASSQ 157
>XP_006723795
          Length = 1480

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AYRASSQ 8
           +YRASSQ
Sbjct: 141 SYRASSQ 147
>XP_006723794
          Length = 1490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AYRASSQ 8
           +YRASSQ
Sbjct: 151 SYRASSQ 157
>XP_006723793
          Length = 1513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AYRASSQ 8
           +YRASSQ
Sbjct: 123 SYRASSQ 129
>XP_006723792
          Length = 1513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AYRASSQ 8
           +YRASSQ
Sbjct: 123 SYRASSQ 129
>XP_006723791
          Length = 1531

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AYRASSQ 8
           +YRASSQ
Sbjct: 141 SYRASSQ 147
>XP_006723790
          Length = 1540

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AYRASSQ 8
           +YRASSQ
Sbjct: 150 SYRASSQ 156
>XP_005273177
          Length = 644

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CAYRASSQ 8
           C +R+SSQ
Sbjct: 178 CPFRSSSQ 185
>NP_001265577
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CAYRASSQ 8
           C +RASS+
Sbjct: 441 CGHRASSR 448
>NP_001139811
          Length = 285

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  CAYRAS 6
          CA+RAS
Sbjct: 49 CAFRAS 54
>NP_775929
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AYRASSQM 9
           AYR SSQ+
Sbjct: 310 AYRYSSQL 317
>NP_001265576
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CAYRASSQ 8
           C +RASS+
Sbjct: 441 CGHRASSR 448
>NP_005332
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CAYRASSQ 8
           C +RASS+
Sbjct: 441 CGHRASSR 448
>XP_005265147
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AYRASSQM 9
           AYR SSQ+
Sbjct: 310 AYRYSSQL 317
>XP_006713180
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AYRASSQM 9
           AYR SSQ+
Sbjct: 310 AYRYSSQL 317
>XP_006710655
          Length = 346

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CAYRASSQ 8
           C +RASS+
Sbjct: 99  CGHRASSR 106
>NP_057510
          Length = 739

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CAYRASSQM 9
           CA+  SSQ+
Sbjct: 435 CAWSESSQL 443
>XP_005261684
          Length = 739

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CAYRASSQM 9
           CA+  SSQ+
Sbjct: 435 CAWSESSQL 443
>NP_005006
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YRASSQM 9
           Y+ASS+M
Sbjct: 256 YKASSEM 262
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.113    0.351 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 386,624
Number of extensions: 302
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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