Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              18    1482
NP_001156602                                                           17    3301
NP_001030008                                                           17    3301
NP_663629                                                              17    3301
NP_001156593                                                           17    3301
NP_001156594                                                           17    3301
NP_004317                                                              17    4312
NP_001010870                                                           17    4312
NP_001161831                                                           17    4312
XP_005277475                                                           17    4312
XP_005277474                                                           17    4312
XP_006711546                                                           17    4312
XP_005273028                                                           17    4312
NP_055501                                                              16    5631
XP_005259501                                                           16    5631
NP_001004758                                                           16    5631
NP_037451                                                              16    7355
NP_036246                                                              16    7355
NP_001157862                                                           16    7355
NP_001157861                                                           16    7355
NP_001157860                                                           16    7355
NP_001157859                                                           16    7355
NP_001157858                                                           16    7355
NP_057424                                                              16    7355
XP_003960647                                                           16    7355
XP_003403507                                                           16    7355
XP_006710653                                                           16    7355
NP_001004698                                                           16    7355
NP_037367                                                              15    9605
NP_001093882                                                           15    9605
NP_861420                                                              15    9605
NP_005516                                                              15    9605
NP_001193670                                                           15    9605
NP_056190                                                              15    9605
NP_955749                                                              15    9605
NP_055919                                                              15    9605
NP_055613                                                              15    9605
NP_055760                                                              15    9605
XP_005273907                                                           15    9605
XP_005273906                                                           15    9605
XP_006714827                                                           15    9605
XP_005268453                                                           15    9605
XP_005268452                                                           15    9605
XP_005268451                                                           15    9605
XP_005268450                                                           15    9605
XP_005268449                                                           15    9605
XP_006714826                                                           15    9605
XP_005248351                                                           15    9605
XP_005248350                                                           15    9605
XP_005248349                                                           15    9605
XP_005248348                                                           15    9605
>NP_000312
          Length = 928

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   CALEVVM 7
           CALEVVM
Sbjct: 489 CALEVVM 495
>NP_001156602
          Length = 261

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   CALEVVM 7
           CALEVV+
Sbjct: 138 CALEVVL 144
>NP_001030008
          Length = 236

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   CALEVVM 7
           CALEVV+
Sbjct: 161 CALEVVL 167
>NP_663629
          Length = 284

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   CALEVVM 7
           CALEVV+
Sbjct: 161 CALEVVL 167
>NP_001156593
          Length = 251

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   CALEVVM 7
           CALEVV+
Sbjct: 161 CALEVVL 167
>NP_001156594
          Length = 228

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   CALEVVM 7
           CALEVV+
Sbjct: 138 CALEVVL 144
>NP_004317
          Length = 1426

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CALEVVMVH 9
           C+L+ +MVH
Sbjct: 495 CSLQDMMVH 503
>NP_001010870
          Length = 2096

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   CALEVVMVH 9
           C L+V+++H
Sbjct: 169 CVLDVLLLH 177
>NP_001161831
          Length = 2066

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   CALEVVMVH 9
           C L+V+++H
Sbjct: 169 CVLDVLLLH 177
>XP_005277475
          Length = 1352

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CALEVVMVH 9
           C+L+ +MVH
Sbjct: 421 CSLQDMMVH 429
>XP_005277474
          Length = 1414

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CALEVVMVH 9
           C+L+ +MVH
Sbjct: 495 CSLQDMMVH 503
>XP_006711546
          Length = 1426

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CALEVVMVH 9
           C+L+ +MVH
Sbjct: 495 CSLQDMMVH 503
>XP_005273028
          Length = 1426

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CALEVVMVH 9
           C+L+ +MVH
Sbjct: 495 CSLQDMMVH 503
>NP_055501
          Length = 1070

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           C L VV VH
Sbjct: 170 CFLTVVQVH 178
>XP_005259501
          Length = 1070

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           C L VV VH
Sbjct: 170 CFLTVVQVH 178
>NP_001004758
          Length = 323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   CALEVVMVH 9
           C L++V +H
Sbjct: 105 CLLQMVFIH 113
>NP_037451
          Length = 338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  CALEVVMVH 9
          CA+ V+ VH
Sbjct: 87 CAVAVLAVH 95
>NP_036246
          Length = 242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  CALEVVMVH 9
          CA  V+M H
Sbjct: 81 CAFVVLMAH 89
>NP_001157862
          Length = 827

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVVMVH 9
           E+VMVH
Sbjct: 395 EIVMVH 400
>NP_001157861
          Length = 1391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVVMVH 9
           E+VMVH
Sbjct: 395 EIVMVH 400
>NP_001157860
          Length = 1504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVVMVH 9
           E+VMVH
Sbjct: 395 EIVMVH 400
>NP_001157859
          Length = 1509

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVVMVH 9
           E+VMVH
Sbjct: 395 EIVMVH 400
>NP_001157858
          Length = 1609

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVVMVH 9
           E+VMVH
Sbjct: 395 EIVMVH 400
>NP_057424
          Length = 1601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   EVVMVH 9
           E+VMVH
Sbjct: 395 EIVMVH 400
>XP_003960647
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           CAL  +M H
Sbjct: 244 CALVALMAH 252
>XP_003403507
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           CAL  +M H
Sbjct: 244 CALVALMAH 252
>XP_006710653
          Length = 211

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  CALEVVMVH 9
          CA+ V+ VH
Sbjct: 87 CAVAVLAVH 95
>NP_001004698
          Length = 320

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           C L VVM H
Sbjct: 112 CVLLVVMSH 120
>NP_037367
          Length = 1374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           C L++ M H
Sbjct: 891 CLLQLAMTH 899
>NP_001093882
          Length = 1337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           C L++ M H
Sbjct: 854 CLLQLAMTH 862
>NP_861420
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   CALEVV 6
           CALE++
Sbjct: 241 CALEII 246
>NP_005516
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   CALEVV 6
           CALE++
Sbjct: 241 CALEII 246
>NP_001193670
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   CALEVV 6
           CALE++
Sbjct: 241 CALEII 246
>NP_056190
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   CALEVVMVH 9
           C+L+ V++H
Sbjct: 641 CSLQDVLLH 649
>NP_955749
          Length = 884

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   CALEVVMVH 9
           C+L+ V++H
Sbjct: 641 CSLQDVLLH 649
>NP_055919
          Length = 1938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   CALEVVM 7
           C+LEV++
Sbjct: 811 CSLEVIL 817
>NP_055613
          Length = 1056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  ALEVVMVH 9
          ALE V +H
Sbjct: 53 ALEAVFIH 60
>NP_055760
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CALEVVMVH 9
          C  EVV VH
Sbjct: 30 CKGEVVRVH 38
>XP_005273907
          Length = 1930

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   CALEVVM 7
           C+LEV++
Sbjct: 811 CSLEVIL 817
>XP_005273906
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   CALEVVM 7
           C+LEV++
Sbjct: 811 CSLEVIL 817
>XP_006714827
          Length = 572

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CALEVVMVH 9
          C  EVV VH
Sbjct: 30 CKGEVVRVH 38
>XP_005268453
          Length = 572

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CALEVVMVH 9
          C  EVV VH
Sbjct: 30 CKGEVVRVH 38
>XP_005268452
          Length = 588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CALEVVMVH 9
          C  EVV VH
Sbjct: 30 CKGEVVRVH 38
>XP_005268451
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CALEVVMVH 9
          C  EVV VH
Sbjct: 30 CKGEVVRVH 38
>XP_005268450
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CALEVVMVH 9
          C  EVV VH
Sbjct: 30 CKGEVVRVH 38
>XP_005268449
          Length = 650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CALEVVMVH 9
          C  EVV VH
Sbjct: 30 CKGEVVRVH 38
>XP_006714826
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  CALEVVMVH 9
          C  EVV VH
Sbjct: 30 CKGEVVRVH 38
>XP_005248351
          Length = 1306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           C L++ M H
Sbjct: 823 CLLQLAMTH 831
>XP_005248350
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           C L++ M H
Sbjct: 860 CLLQLAMTH 868
>XP_005248349
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           C L++ M H
Sbjct: 883 CLLQLAMTH 891
>XP_005248348
          Length = 1374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1   CALEVVMVH 9
           C L++ M H
Sbjct: 891 CLLQLAMTH 899
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.138    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 388,606
Number of extensions: 698
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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