Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_056153                                                              16    5631
XP_006723795                                                           16    5631
XP_006723794                                                           16    5631
XP_006723793                                                           16    5631
XP_006723792                                                           16    5631
XP_006723791                                                           16    5631
XP_006723790                                                           16    5631
NP_775929                                                              16    7355
XP_005265147                                                           16    7355
XP_006713180                                                           16    7355
NP_001007076                                                           15    9605
NP_057074                                                              15    9605
NP_950240                                                              15    9605
NP_835224                                                              15    9605
NP_653291                                                              15    9605
NP_954672                                                              15    9605
NP_057669                                                              15    9605
XP_006723107                                                           15    9605
XP_005259626                                                           15    9605
XP_005262712                                                           15    9605
XP_005262711                                                           15    9605
XP_005247001                                                           15    9605
NP_005006                                                              15    9605
>NP_056153
          Length = 1541

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AYRASSQ 7
           +YRASSQ
Sbjct: 151 SYRASSQ 157
>XP_006723795
          Length = 1480

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AYRASSQ 7
           +YRASSQ
Sbjct: 141 SYRASSQ 147
>XP_006723794
          Length = 1490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AYRASSQ 7
           +YRASSQ
Sbjct: 151 SYRASSQ 157
>XP_006723793
          Length = 1513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AYRASSQ 7
           +YRASSQ
Sbjct: 123 SYRASSQ 129
>XP_006723792
          Length = 1513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AYRASSQ 7
           +YRASSQ
Sbjct: 123 SYRASSQ 129
>XP_006723791
          Length = 1531

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AYRASSQ 7
           +YRASSQ
Sbjct: 141 SYRASSQ 147
>XP_006723790
          Length = 1540

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AYRASSQ 7
           +YRASSQ
Sbjct: 150 SYRASSQ 156
>NP_775929
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AYRASSQM 8
           AYR SSQ+
Sbjct: 310 AYRYSSQL 317
>XP_005265147
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AYRASSQM 8
           AYR SSQ+
Sbjct: 310 AYRYSSQL 317
>XP_006713180
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AYRASSQM 8
           AYR SSQ+
Sbjct: 310 AYRYSSQL 317
>NP_001007076
          Length = 709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  YRASSQME 9
          YR SSQ++
Sbjct: 68 YRRSSQLD 75
>NP_057074
          Length = 755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YRASSQME 9
           YR SSQ++
Sbjct: 114 YRRSSQLD 121
>NP_950240
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YRASSQME 9
           YR SSQ++
Sbjct: 114 YRRSSQLD 121
>NP_835224
          Length = 696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YRASSQME 9
           YR +SQ+E
Sbjct: 364 YRQASQLE 371
>NP_653291
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AYRASSQME 9
           A+R SSQ++
Sbjct: 375 AFRVSSQLK 383
>NP_954672
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YRASSQME 9
           YRASS+++
Sbjct: 202 YRASSRLK 209
>NP_057669
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YRASSQME 9
           YRASS+++
Sbjct: 226 YRASSRLK 233
>XP_006723107
          Length = 548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AYRASSQME 9
           A+R SSQ++
Sbjct: 406 AFRVSSQLK 414
>XP_005259626
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   YRASSQME 9
           YRASS+++
Sbjct: 194 YRASSRLK 201
>XP_005262712
          Length = 789

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YRASSQME 9
           YR SSQ++
Sbjct: 148 YRRSSQLD 155
>XP_005262711
          Length = 791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YRASSQME 9
           YR SSQ++
Sbjct: 148 YRRSSQLD 155
>XP_005247001
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   YRASSQME 9
           YR +SQ+E
Sbjct: 364 YRQASQLE 371
>NP_005006
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   YRASSQM 8
           Y+ASS+M
Sbjct: 256 YKASSEM 262
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.103    0.245 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 340,125
Number of extensions: 303
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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