Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_113662                                                              18    1135
XP_005253855                                                           18    1135
XP_005253854                                                           18    1135
XP_005253853                                                           18    1135
XP_006721884                                                           17    2528
XP_005257286                                                           17    2528
NP_000207                                                              17    3301
XP_005274558                                                           17    3301
NP_536799                                                              17    4312
NP_821079                                                              17    4312
NP_079511                                                              17    4312
NP_003738                                                              17    4312
XP_005270242                                                           17    4312
XP_006716326                                                           17    4312
NP_061846                                                              16    5631
NP_714963                                                              16    5631
NP_000675                                                              16    5631
NP_055884                                                              16    5631
NP_001258549                                                           16    5631
NP_065891                                                              16    5631
NP_005551                                                              16    5631
XP_006723859                                                           16    5631
XP_005255914                                                           16    5631
XP_005255913                                                           16    5631
XP_005255912                                                           16    5631
XP_006721234                                                           16    5631
XP_006714344                                                           16    5631
XP_005263218                                                           16    5631
XP_005263217                                                           16    5631
XP_005263216                                                           16    5631
XP_005263215                                                           16    5631
XP_005263213                                                           16    5631
NP_005021                                                              16    7355
NP_852466                                                              16    7355
NP_443100                                                              16    7355
NP_001193594                                                           16    7355
XP_006724942                                                           16    7355
XP_005265160                                                           16    7355
XP_006713189                                                           16    7355
XP_005265159                                                           16    7355
XP_006711694                                                           16    7355
XP_005245671                                                           16    7355
XP_006710331                                                           16    7355
NP_002011                                                              15    9605
NP_002323                                                              15    9605
NP_891555                                                              15    9605
NP_001035373                                                           15    9605
NP_073617                                                              15    9605
XP_005259205                                                           15    9605
XP_005259203                                                           15    9605
XP_006723383                                                           15    9605
XP_006719839                                                           15    9605
XP_005252850                                                           15    9605
XP_006710034                                                           15    9605
NP_699202                                                              15    9605
NP_001004474                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AVSAGCLA 8
            AVSAGCLA
Sbjct: 2875 AVSAGCLA 2882
>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    AVSAGCLA 8
            AVSAGCLA
Sbjct: 2875 AVSAGCLA 2882
>NP_113662
          Length = 281

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSAGCLAR 9
           +SAGCL+R
Sbjct: 184 ISAGCLSR 191
>XP_005253855
          Length = 279

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSAGCLAR 9
           +SAGCL+R
Sbjct: 184 ISAGCLSR 191
>XP_005253854
          Length = 281

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSAGCLAR 9
           +SAGCL+R
Sbjct: 184 ISAGCLSR 191
>XP_005253853
          Length = 294

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSAGCLAR 9
           +SAGCL+R
Sbjct: 184 ISAGCLSR 191
>XP_006721884
          Length = 461

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AVSAGCLAR 9
           A S+GCL R
Sbjct: 448 AASSGCLTR 456
>XP_005257286
          Length = 562

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AVSAGCLAR 9
           A S+GCL R
Sbjct: 549 AASSGCLTR 557
>NP_000207
          Length = 680

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  AVSAGCLA 8
          A S+GCLA
Sbjct: 17 AASSGCLA 24
>XP_005274558
          Length = 420

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  AVSAGCLA 8
          A S+GCLA
Sbjct: 17 AASSGCLA 24
>NP_536799
          Length = 445

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  SAGCLA 8
          SAGCLA
Sbjct: 21 SAGCLA 26
>NP_821079
          Length = 581

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AVSAGCL 7
          ++SAGCL
Sbjct: 58 SISAGCL 64
>NP_079511
          Length = 1166

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AVSAGCLAR 9
           A   GCLAR
Sbjct: 652 AAKKGCLAR 660
>NP_003738
          Length = 1327

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AVSAGCLAR 9
           A   GCLAR
Sbjct: 810 AAKKGCLAR 818
>XP_005270242
          Length = 734

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AVSAGCLAR 9
           A   GCLAR
Sbjct: 220 AAKKGCLAR 228
>XP_006716326
          Length = 1316

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AVSAGCLAR 9
           A   GCLAR
Sbjct: 810 AAKKGCLAR 818
>NP_061846
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VSAGCLA 8
          VSAGC A
Sbjct: 77 VSAGCYA 83
>NP_714963
          Length = 92

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VSAGCLA 8
          VSAGC A
Sbjct: 47 VSAGCYA 53
>NP_000675
          Length = 477

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SAGCLAR 9
           ++GCLAR
Sbjct: 411 ASGCLAR 417
>NP_055884
          Length = 1284

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AGCLAR 9
            AGC+AR
Sbjct: 1093 AGCMAR 1098
>NP_001258549
          Length = 1224

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AGCLAR 9
            AGC+AR
Sbjct: 1033 AGCMAR 1038
>NP_065891
          Length = 1114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVSAGCL 7
           A SAGCL
Sbjct: 628 ACSAGCL 634
>NP_005551
          Length = 3695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    VSAGCLA 8
            VSAGC A
Sbjct: 3111 VSAGCTA 3117
>XP_006723859
          Length = 3700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    VSAGCLA 8
            VSAGC A
Sbjct: 3111 VSAGCTA 3117
>XP_005255914
          Length = 1167

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AGCLAR 9
           AGC+AR
Sbjct: 976 AGCMAR 981
>XP_005255913
          Length = 1224

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AGCLAR 9
            AGC+AR
Sbjct: 1033 AGCMAR 1038
>XP_005255912
          Length = 1286

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AGCLAR 9
            AGC+AR
Sbjct: 1095 AGCMAR 1100
>XP_006721234
          Length = 1299

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AGCLAR 9
            AGC+AR
Sbjct: 1108 AGCMAR 1113
>XP_006714344
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVSAGCL 7
           A SAGCL
Sbjct: 274 ACSAGCL 280
>XP_005263218
          Length = 1040

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVSAGCL 7
           A SAGCL
Sbjct: 681 ACSAGCL 687
>XP_005263217
          Length = 1061

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVSAGCL 7
           A SAGCL
Sbjct: 681 ACSAGCL 687
>XP_005263216
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVSAGCL 7
           A SAGCL
Sbjct: 651 ACSAGCL 657
>XP_005263215
          Length = 1144

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVSAGCL 7
           A SAGCL
Sbjct: 658 ACSAGCL 664
>XP_005263213
          Length = 1167

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVSAGCL 7
           A SAGCL
Sbjct: 681 ACSAGCL 687
>NP_005021
          Length = 603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  AVSAGCLAR 9
          AV+AG LAR
Sbjct: 4  AVTAGKLAR 12
>NP_852466
          Length = 1031

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AVSAGCLA 8
           AVS+GC A
Sbjct: 335 AVSSGCPA 342
>NP_443100
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VSAGCLAR 9
           ++ GCLAR
Sbjct: 182 LALGCLAR 189
>NP_001193594
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VSAGCLAR 9
           ++ GCLAR
Sbjct: 182 LALGCLAR 189
>XP_006724942
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAGCLA 8
           SAGC+A
Sbjct: 595 SAGCMA 600
>XP_005265160
          Length = 826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AVSAGCLA 8
           AVS+GC A
Sbjct: 130 AVSSGCPA 137
>XP_006713189
          Length = 1019

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AVSAGCLA 8
           AVS+GC A
Sbjct: 323 AVSSGCPA 330
>XP_005265159
          Length = 1031

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AVSAGCLA 8
           AVS+GC A
Sbjct: 335 AVSSGCPA 342
>XP_006711694
          Length = 596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VSAGCLAR 9
           ++ GCLAR
Sbjct: 165 LALGCLAR 172
>XP_005245671
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VSAGCLAR 9
           ++ GCLAR
Sbjct: 182 LALGCLAR 189
>XP_006710331
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAGCLA 8
           SAGC+A
Sbjct: 595 SAGCMA 600
>NP_002011
          Length = 1298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GCLAR 9
            GCLAR
Sbjct: 1238 GCLAR 1242
>NP_002323
          Length = 4544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    AVSAGCL 7
            +++AGCL
Sbjct: 2807 SIAAGCL 2813
>NP_891555
          Length = 1363

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GCLAR 9
            GCLAR
Sbjct: 1238 GCLAR 1242
>NP_001035373
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AVSAGCLAR 9
           A  A CLAR
Sbjct: 273 ASGASCLAR 281
>NP_073617
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAGCLA 8
           SAGC+A
Sbjct: 287 SAGCVA 292
>XP_005259205
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AVSAGCLAR 9
           A  A CLAR
Sbjct: 273 ASGASCLAR 281
>XP_005259203
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AVSAGCLAR 9
           A  A CLAR
Sbjct: 276 ASGASCLAR 284
>XP_006723383
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   AVSAGCLAR 9
           A  A CLAR
Sbjct: 324 ASGASCLAR 332
>XP_006719839
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   GCLAR 9
           GCLAR
Sbjct: 382 GCLAR 386
>XP_005252850
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  AVSAGCLAR 9
          + S+GC+A+
Sbjct: 5  SASSGCVAK 13
>XP_006710034
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VSAGCLA 8
           VSAGC +
Sbjct: 214 VSAGCFS 220
>NP_699202
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   GCLAR 9
           GCLAR
Sbjct: 505 GCLAR 509
>NP_001004474
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VSAGCL 7
           V+AGCL
Sbjct: 220 VAAGCL 225
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.128    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 546,045
Number of extensions: 579
Number of successful extensions: 58
Number of sequences better than 10000.0: 58
Number of HSP's gapped: 58
Number of HSP's successfully gapped: 58
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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