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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1935
NP_001075109                                                           18    1935
NP_001171482                                                           17    4312
NP_001171481                                                           17    4312
NP_003495                                                              17    4312
XP_005261342                                                           17    4312
XP_005261343                                                           17    4312
NP_078613                                                              16    7355
NP_060608                                                              16    7355
NP_001127887                                                           16    7355
NP_004176                                                              16    7355
NP_619515                                                              15    9605
NP_001034230                                                           15    9605
NP_005059                                                              15    9605
NP_071766                                                              15    9605
XP_005267157                                                           15    9605
XP_006711548                                                           15    9605

>NP_008835
          Length = 4128

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AVQIRATQQ 9
            A QIRATQQ
Sbjct: 2632 AGQIRATQQ 2640



>NP_001075109
          Length = 4097

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AVQIRATQQ 9
            A QIRATQQ
Sbjct: 2632 AGQIRATQQ 2640



>NP_001171482
          Length = 520

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QIRATQQ 9
           Q+RATQQ
Sbjct: 376 QLRATQQ 382



>NP_001171481
          Length = 598

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QIRATQQ 9
           Q+RATQQ
Sbjct: 454 QLRATQQ 460



>NP_003495
          Length = 566

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QIRATQQ 9
           Q+RATQQ
Sbjct: 422 QLRATQQ 428



>XP_005261342
          Length = 598

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QIRATQQ 9
           Q+RATQQ
Sbjct: 454 QLRATQQ 460



>XP_005261343
          Length = 567

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QIRATQQ 9
           Q+RATQQ
Sbjct: 422 QLRATQQ 428



>NP_078613
          Length = 391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AVQIRATQ 8
           AVQ+ ATQ
Sbjct: 272 AVQVMATQ 279



>NP_060608
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  VQIRATQ 8
          VQIRAT+
Sbjct: 40 VQIRATE 46



>NP_001127887
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  VQIRATQ 8
          VQIRAT+
Sbjct: 40 VQIRATE 46



>NP_004176
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   AVQIRATQ 8
           AVQ+ ATQ
Sbjct: 253 AVQVMATQ 260



>NP_619515
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AVQIRATQ 8
           A Q+RAT+
Sbjct: 261 AAQVRATE 268



>NP_001034230
          Length = 2365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   IRATQQ 9
           IRATQQ
Sbjct: 807 IRATQQ 812



>NP_005059
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   IRATQQ 9
           IRATQQ
Sbjct: 568 IRATQQ 573



>NP_071766
          Length = 397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QIRATQQ 9
           QIR TQQ
Sbjct: 219 QIRETQQ 225



>XP_005267157
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   IRATQQ 9
           IRATQQ
Sbjct: 568 IRATQQ 573



>XP_006711548
          Length = 181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3 QIRATQQ 9
         QIR TQQ
Sbjct: 3 QIRETQQ 9



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.117    0.272 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 236,506
Number of extensions: 188
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)