Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     665
NP_001075109                                                           19     665
NP_077017                                                              18    1135
NP_004937                                                              16    7355
NP_000072                                                              15    9605
NP_001165088                                                           15    9605
NP_001165074                                                           15    9605
NP_001165077                                                           15    9605
NP_062552                                                              15    9605
NP_001035845                                                           15    9605
NP_006027                                                              15    9605
NP_001035844                                                           15    9605
NP_653285                                                              15    9605
NP_079435                                                              15    9605
NP_001165084                                                           15    9605
XP_006723081                                                           15    9605
XP_005264717                                                           15    9605
XP_005264716                                                           15    9605
XP_005264715                                                           15    9605
XP_005264714                                                           15    9605
XP_005264712                                                           15    9605
XP_005264711                                                           15    9605
XP_006712215                                                           15    9605
XP_006712214                                                           15    9605
XP_005264710                                                           15    9605
XP_005273087                                                           15    9605
XP_005273086                                                           15    9605
XP_005273085                                                           15    9605
XP_005273084                                                           15    9605
NP_001005190                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AVLNSMLDQ 9
            AVLN MLDQ
Sbjct: 1595 AVLNGMLDQ 1603
>NP_001075109
          Length = 4097

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AVLNSMLDQ 9
            AVLN MLDQ
Sbjct: 1595 AVLNGMLDQ 1603
>NP_077017
          Length = 83

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  AVLNSMLDQ 9
          A +NSMLDQ
Sbjct: 29 AAINSMLDQ 37
>NP_004937
          Length = 1830

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AVLNSMLDQ 9
           A+LN M DQ
Sbjct: 942 AILNQMGDQ 950
>NP_000072
          Length = 3801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNSMLDQ 9
          LNS++DQ
Sbjct: 58 LNSIIDQ 64
>NP_001165088
          Length = 638

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 499 VLNTMMD 505
>NP_001165074
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 588 VLNTMMD 594
>NP_001165077
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 588 VLNTMMD 594
>NP_062552
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LNSMLDQ 9
           LNS+LD+
Sbjct: 197 LNSLLDE 203
>NP_001035845
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 522 VLNTMMD 528
>NP_006027
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 588 VLNTMMD 594
>NP_001035844
          Length = 665

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 526 VLNTMMD 532
>NP_653285
          Length = 535

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNSMLDQ 9
          +NSML+Q
Sbjct: 75 INSMLEQ 81
>NP_079435
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AVLNSMLDQ 9
           AVLN  L+Q
Sbjct: 208 AVLNEYLEQ 216
>NP_001165084
          Length = 638

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 499 VLNTMMD 505
>XP_006723081
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNSMLDQ 9
          +NSML+Q
Sbjct: 75 INSMLEQ 81
>XP_005264717
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 499 VLNTMMD 505
>XP_005264716
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 499 VLNTMMD 505
>XP_005264715
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 499 VLNTMMD 505
>XP_005264714
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 588 VLNTMMD 594
>XP_005264712
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 588 VLNTMMD 594
>XP_005264711
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 588 VLNTMMD 594
>XP_006712215
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 588 VLNTMMD 594
>XP_006712214
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 588 VLNTMMD 594
>XP_005264710
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VLNSMLD 8
           VLN+M+D
Sbjct: 588 VLNTMMD 594
>XP_005273087
          Length = 2788

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNSMLDQ 9
          LNS++DQ
Sbjct: 58 LNSIIDQ 64
>XP_005273086
          Length = 3264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNSMLDQ 9
          LNS++DQ
Sbjct: 58 LNSIIDQ 64
>XP_005273085
          Length = 3755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNSMLDQ 9
          LNS++DQ
Sbjct: 58 LNSIIDQ 64
>XP_005273084
          Length = 3801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LNSMLDQ 9
          LNS++DQ
Sbjct: 58 LNSIIDQ 64
>NP_001005190
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AVLNSML 7
           +VLNSML
Sbjct: 152 SVLNSML 158
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.125    0.303 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 365,562
Number of extensions: 643
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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