Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           20     509
NP_000242                                                              20     509
XP_005264389                                                           20     509
NP_775489                                                              17    3301
XP_005268699                                                           17    3301
NP_057287                                                              16    5631
NP_077747                                                              16    5631
NP_001035723                                                           16    5631
XP_005250066                                                           16    5631
XP_005250064                                                           16    5631
XP_005250063                                                           16    5631
XP_005250062                                                           16    5631
XP_005250061                                                           16    5631
XP_006716084                                                           16    5631
XP_005250060                                                           16    5631
XP_005250059                                                           16    5631
NP_055192                                                              16    7355
NP_001123359                                                           16    7355
NP_004170                                                              16    7355
XP_005250326                                                           16    7355
NP_653197                                                              15    9605
NP_001275710                                                           15    9605
NP_938051                                                              15    9605
NP_938052                                                              15    9605
NP_001275711                                                           15    9605
NP_001275712                                                           15    9605
NP_859056                                                              15    9605
NP_001166251                                                           15    9605
NP_001166250                                                           15    9605
XP_006720100                                                           15    9605
XP_006720099                                                           15    9605
XP_006720098                                                           15    9605
XP_005247190                                                           15    9605
XP_006713571                                                           15    9605

>NP_001245210
          Length = 868

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AVKALNLFQ 9
           AV+ALNLFQ
Sbjct: 240 AVRALNLFQ 248



>NP_000242
          Length = 934

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AVKALNLFQ 9
           AV+ALNLFQ
Sbjct: 306 AVRALNLFQ 314



>XP_005264389
          Length = 921

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AVKALNLFQ 9
           AV+ALNLFQ
Sbjct: 306 AVRALNLFQ 314



>NP_775489
          Length = 490

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  VKALNLFQ 9
          VKAL LFQ
Sbjct: 78 VKALRLFQ 85



>XP_005268699
          Length = 496

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  VKALNLFQ 9
          VKAL LFQ
Sbjct: 84 VKALRLFQ 91



>NP_057287
          Length = 569

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 446 IKALNIF 452



>NP_077747
          Length = 328

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 205 IKALNIF 211



>NP_001035723
          Length = 525

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 402 IKALNIF 408



>XP_005250066
          Length = 358

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 185 IKALNIF 191



>XP_005250064
          Length = 444

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 321 IKALNIF 327



>XP_005250063
          Length = 495

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 322 IKALNIF 328



>XP_005250062
          Length = 568

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 445 IKALNIF 451



>XP_005250061
          Length = 575

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 402 IKALNIF 408



>XP_006716084
          Length = 615

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 442 IKALNIF 448



>XP_005250060
          Length = 618

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 445 IKALNIF 451



>XP_005250059
          Length = 619

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KALN+F
Sbjct: 446 IKALNIF 452



>NP_055192
          Length = 621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KA+NLF
Sbjct: 463 IKAVNLF 469



>NP_001123359
          Length = 568

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KA+NLF
Sbjct: 410 IKAVNLF 416



>NP_004170
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  VKALNLFQ 9
          +KAL +FQ
Sbjct: 32 IKALKIFQ 39



>XP_005250326
          Length = 475

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VKALNLF 8
           +KA+NLF
Sbjct: 317 IKAVNLF 323



>NP_653197
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VKALNLFQ 9
           V ALNLF+
Sbjct: 275 VTALNLFK 282



>NP_001275710
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VKALNLFQ 9
           V ALNLF+
Sbjct: 291 VTALNLFK 298



>NP_938051
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VKALNLFQ 9
           V ALNLF+
Sbjct: 265 VTALNLFK 272



>NP_938052
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VKALNLFQ 9
           V ALNLF+
Sbjct: 235 VTALNLFK 242



>NP_001275711
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VKALNLFQ 9
          V ALNLF+
Sbjct: 77 VTALNLFK 84



>NP_001275712
          Length = 276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VKALNLFQ 9
          V ALNLF+
Sbjct: 36 VTALNLFK 43



>NP_859056
          Length = 104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  AVKALNLFQ 9
          AVK L LF+
Sbjct: 4  AVKVLQLFK 12



>NP_001166251
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVKALNL 7
           A+KA+NL
Sbjct: 236 AIKAINL 242



>NP_001166250
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVKALNL 7
           A+KA+NL
Sbjct: 236 AIKAINL 242



>XP_006720100
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VKALNLFQ 9
           V ALNLF+
Sbjct: 235 VTALNLFK 242



>XP_006720099
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VKALNLFQ 9
           V ALNLF+
Sbjct: 265 VTALNLFK 272



>XP_006720098
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VKALNLFQ 9
           V ALNLF+
Sbjct: 265 VTALNLFK 272



>XP_005247190
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVKALNL 7
           A+KA+NL
Sbjct: 175 AIKAINL 181



>XP_006713571
          Length = 446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVKALNL 7
           A+KA+NL
Sbjct: 218 AIKAINL 224



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.136    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 275,097
Number of extensions: 326
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)