Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     665
NP_000242                                                              19     665
XP_005264389                                                           19     665
NP_004035                                                              17    2528
XP_006712608                                                           17    2528
NP_001094348                                                           17    4312
NP_031392                                                              16    5631
NP_001007268                                                           16    5631
NP_001182570                                                           16    5631
XP_005246452                                                           16    5631
XP_006712432                                                           16    5631
XP_005246450                                                           16    5631
XP_005246449                                                           16    5631
NP_996763                                                              16    5631
NP_059107                                                              16    7355
NP_075013                                                              16    7355
NP_075014                                                              16    7355
NP_000335                                                              16    7355
NP_075015                                                              16    7355
NP_075012                                                              16    7355
NP_055452                                                              16    7355
NP_001139311                                                           16    7355
NP_055637                                                              16    7355
XP_005276733                                                           16    7355
XP_005248632                                                           16    7355
XP_005276832                                                           16    7355
XP_005259517                                                           16    7355
XP_006716981                                                           16    7355
XP_006716980                                                           16    7355
XP_005272572                                                           16    7355
XP_005272573                                                           16    7355
XP_005251384                                                           16    7355
XP_006716547                                                           16    7355
XP_006714740                                                           16    7355
XP_005263436                                                           16    7355
XP_005263435                                                           16    7355
NP_001139310                                                           16    7355
NP_149350                                                              16    7355
NP_078796                                                              15    9605
NP_056076                                                              15    9605
NP_001005850                                                           15    9605
XP_005259440                                                           15    9605
XP_005259439                                                           15    9605
XP_005246909                                                           15    9605
>NP_001245210
          Length = 868

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVASFAHVS 9
           AV SFAHVS
Sbjct: 538 AVVSFAHVS 546
>NP_000242
          Length = 934

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVASFAHVS 9
           AV SFAHVS
Sbjct: 604 AVVSFAHVS 612
>XP_005264389
          Length = 921

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVASFAHVS 9
           AV SFAHVS
Sbjct: 604 AVVSFAHVS 612
>NP_004035
          Length = 592

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   AVASFAHVS 9
           A ASF HVS
Sbjct: 261 AAASFKHVS 269
>XP_006712608
          Length = 533

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   AVASFAHVS 9
           A ASF HVS
Sbjct: 202 AAASFKHVS 210
>NP_001094348
          Length = 719

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   AVASFAHVS 9
           AVA+ AHVS
Sbjct: 656 AVAAAAHVS 664
>NP_031392
          Length = 1463

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VASFAHV 8
           +ASFAH+
Sbjct: 706 LASFAHI 712
>NP_001007268
          Length = 1324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VASFAHV 8
           +ASFAH+
Sbjct: 706 LASFAHI 712
>NP_001182570
          Length = 1461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VASFAHV 8
           +ASFAH+
Sbjct: 706 LASFAHI 712
>XP_005246452
          Length = 776

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  VASFAHV 8
          +ASFAH+
Sbjct: 19 LASFAHI 25
>XP_006712432
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  VASFAHV 8
          +ASFAH+
Sbjct: 29 LASFAHI 35
>XP_005246450
          Length = 1461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VASFAHV 8
           +ASFAH+
Sbjct: 706 LASFAHI 712
>XP_005246449
          Length = 1463

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VASFAHV 8
           +ASFAH+
Sbjct: 706 LASFAHI 712
>NP_996763
          Length = 315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VASFAHV 8
           VAS+AH+
Sbjct: 216 VASYAHI 222
>NP_059107
          Length = 294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVASFAH 7
          AVASF H
Sbjct: 46 AVASFKH 52
>NP_075013
          Length = 282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVASFAH 7
          AVASF H
Sbjct: 46 AVASFKH 52
>NP_075014
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVASFAH 7
          AVASF H
Sbjct: 46 AVASFKH 52
>NP_000335
          Length = 294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVASFAH 7
          AVASF H
Sbjct: 46 AVASFKH 52
>NP_075015
          Length = 250

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVASFAH 7
          AVASF H
Sbjct: 46 AVASFKH 52
>NP_075012
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVASFAH 7
          AVASF H
Sbjct: 46 AVASFKH 52
>NP_055452
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  AVASFAHV 8
          AVASFA V
Sbjct: 72 AVASFADV 79
>NP_001139311
          Length = 348

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  AVASFAHV 8
          AVASFA V
Sbjct: 72 AVASFADV 79
>NP_055637
          Length = 1032

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VASFAHVS 9
           V SFAH++
Sbjct: 946 VPSFAHIN 953
>XP_005276733
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  AVASFAHVS 9
          + +SFAH+S
Sbjct: 62 STSSFAHLS 70
>XP_005248632
          Length = 282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVASFAH 7
          AVASF H
Sbjct: 46 AVASFKH 52
>XP_005276832
          Length = 282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVASFAH 7
          AVASF H
Sbjct: 46 AVASFKH 52
>XP_005259517
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  AVASFAHVS 9
          + +SFAH+S
Sbjct: 62 STSSFAHLS 70
>XP_006716981
          Length = 1053

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFAHVS 9
           SFAH+S
Sbjct: 632 SFAHIS 637
>XP_006716980
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFAHVS 9
           SFAH+S
Sbjct: 638 SFAHIS 643
>XP_005272572
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFAHVS 9
           SFAH+S
Sbjct: 638 SFAHIS 643
>XP_005272573
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFAHVS 9
           SFAH+S
Sbjct: 638 SFAHIS 643
>XP_005251384
          Length = 320

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  AVASFAHV 8
          AVASFA V
Sbjct: 59 AVASFADV 66
>XP_006716547
          Length = 348

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  AVASFAHV 8
          AVASFA V
Sbjct: 72 AVASFADV 79
>XP_006714740
          Length = 282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVASFAH 7
          AVASF H
Sbjct: 46 AVASFKH 52
>XP_005263436
          Length = 1033

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VASFAHVS 9
           V SFAH++
Sbjct: 947 VPSFAHIN 954
>XP_005263435
          Length = 1033

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VASFAHVS 9
           V SFAH++
Sbjct: 947 VPSFAHIN 954
>NP_001139310
          Length = 300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  AVASFAHV 8
          AVASFA V
Sbjct: 39 AVASFADV 46
>NP_149350
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SFAHVS 9
           SFAH+S
Sbjct: 638 SFAHIS 643
>NP_078796
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AVASFAHVS 9
           A  +FAHVS
Sbjct: 148 AKQAFAHVS 156
>NP_056076
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVASFAH 7
           A++SFAH
Sbjct: 464 ALSSFAH 470
>NP_001005850
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ASFAHVS 9
           A+F+HVS
Sbjct: 397 AAFSHVS 403
>XP_005259440
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ASFAHVS 9
           A+F+HVS
Sbjct: 397 AAFSHVS 403
>XP_005259439
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ASFAHVS 9
           A+F+HVS
Sbjct: 397 AAFSHVS 403
>XP_005246909
          Length = 225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AVASFAHVS 9
           A  +FAHVS
Sbjct: 148 AKQAFAHVS 156
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.116    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 427,398
Number of extensions: 601
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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