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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              19     869
XP_006710739                                                           17    2528
XP_005270979                                                           17    2528
NP_056972                                                              17    2528
NP_073568                                                              16    5631
NP_055045                                                              16    5631
NP_079212                                                              16    5631
NP_001247431                                                           16    5631
XP_005245510                                                           16    5631
NP_055836                                                              16    7355
XP_005248755                                                           16    7355
XP_005248754                                                           16    7355
NP_150094                                                              15    9605
NP_005834                                                              15    9605

>NP_005219
          Length = 1210

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ATSSKANKE 9
           ATS KANKE
Sbjct: 750 ATSPKANKE 758



>XP_006710739
          Length = 560

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ATSSKANK 8
           ATS KANK
Sbjct: 311 ATSDKANK 318



>XP_005270979
          Length = 728

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ATSSKANK 8
           ATS KANK
Sbjct: 311 ATSDKANK 318



>NP_056972
          Length = 728

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ATSSKANK 8
           ATS KANK
Sbjct: 311 ATSDKANK 318



>NP_073568
          Length = 243

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TSSKANKE 9
           T+SKA+KE
Sbjct: 193 TASKASKE 200



>NP_055045
          Length = 627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ATSSKANKE 9
          +  SKANKE
Sbjct: 45 SAGSKANKE 53



>NP_079212
          Length = 513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ATSSKANK 8
           ATS +ANK
Sbjct: 462 ATSKRANK 469



>NP_001247431
          Length = 589

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ATSSKANKE 9
          +  SKANKE
Sbjct: 45 SAGSKANKE 53



>XP_005245510
          Length = 242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TSSKANKE 9
           T+SKA+KE
Sbjct: 192 TASKASKE 199



>NP_055836
          Length = 2723

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SSKANKE 9
            SSKAN+E
Sbjct: 2303 SSKANQE 2309



>XP_005248755
          Length = 2465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SSKANKE 9
            SSKAN+E
Sbjct: 2045 SSKANQE 2051



>XP_005248754
          Length = 2583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SSKANKE 9
            SSKAN+E
Sbjct: 2163 SSKANQE 2169



>NP_150094
          Length = 3564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ATSSKAN 7
            ATSSK N
Sbjct: 3380 ATSSKVN 3386



>NP_005834
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TSSKANKE 9
           TSS  NKE
Sbjct: 159 TSSNKNKE 166



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.266   0.0829    0.175 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 281,001
Number of extensions: 297
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 19 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.3 bits)
S2: 28 (15.4 bits)