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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              17    2528
NP_001075109                                                           17    2528
NP_001093321                                                           16    5631
NP_001038945                                                           16    5631
NP_001034450                                                           16    5631
NP_001278310                                                           16    5631
NP_002354                                                              16    5631
NP_803134                                                              16    5631
NP_001136336                                                           16    5631
NP_796379                                                              16    5631
XP_006721417                                                           16    5631
XP_006721416                                                           16    5631
XP_005246247                                                           16    7355

>NP_008835
          Length = 4128

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASRLLELAF 9
            AS LLELAF
Sbjct: 1511 ASGLLELAF 1519



>NP_001075109
          Length = 4097

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASRLLELAF 9
            AS LLELAF
Sbjct: 1511 ASGLLELAF 1519



>NP_001093321
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2  SRLLELA 8
          SRLLELA
Sbjct: 7  SRLLELA 13



>NP_001038945
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2  SRLLELA 8
          SRLLELA
Sbjct: 7  SRLLELA 13



>NP_001034450
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2  SRLLELA 8
          SRLLELA
Sbjct: 7  SRLLELA 13



>NP_001278310
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2  SRLLELA 8
          SRLLELA
Sbjct: 7  SRLLELA 13



>NP_002354
          Length = 347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ASRLLELAF 9
           ASR LEL F
Sbjct: 153 ASRRLELVF 161



>NP_803134
          Length = 347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ASRLLELAF 9
           ASR LEL F
Sbjct: 153 ASRRLELVF 161



>NP_001136336
          Length = 2521

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ASRLLELA 8
           A RLLELA
Sbjct: 785 AERLLELA 792



>NP_796379
          Length = 347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ASRLLELAF 9
           ASR LEL F
Sbjct: 153 ASRRLELVF 161



>XP_006721417
          Length = 2497

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ASRLLELA 8
           A RLLELA
Sbjct: 785 AERLLELA 792



>XP_006721416
          Length = 2504

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ASRLLELA 8
           A RLLELA
Sbjct: 768 AERLLELA 775



>XP_005246247
          Length = 1417

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    RLLELAF 9
            RLL+LAF
Sbjct: 1388 RLLDLAF 1394



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.141    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 319,255
Number of extensions: 234
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)