Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              20     509
NP_005913                                                              20     509
XP_005267046                                                           20     509
XP_005267045                                                           20     509
NP_579878                                                              17    2528
NP_015627                                                              17    2528
NP_579890                                                              17    2528
NP_579889                                                              17    2528
NP_579877                                                              17    2528
NP_001035889                                                           17    2528
XP_006713977                                                           17    2528
XP_005248062                                                           17    2528
XP_005248058                                                           17    2528
NP_001167555                                                           17    4312
NP_037406                                                              17    4312
XP_006717836                                                           17    4312
XP_006717835                                                           17    4312
XP_006717834                                                           17    4312
XP_006717833                                                           17    4312
NP_001186306                                                           16    5631
NP_055977                                                              16    5631
NP_001159777                                                           16    5631
NP_056512                                                              16    5631
NP_001011655                                                           16    5631
NP_001159778                                                           16    5631
NP_612408                                                              16    5631
XP_006713887                                                           16    5631
XP_006713886                                                           16    5631
XP_005269429                                                           16    5631
XP_005269428                                                           16    5631
NP_004068                                                              16    7355
NP_004057                                                              15    9605
NP_689914                                                              15    9605
NP_004335                                                              15    9605
XP_006726390                                                           15    9605
XP_006726389                                                           15    9605
XP_006726388                                                           15    9605
XP_006726387                                                           15    9605
XP_006711142                                                           15    9605
XP_006711141                                                           15    9605
XP_006711140                                                           15    9605
XP_006710131                                                           15    9605
XP_006710130                                                           15    9605
XP_006710129                                                           15    9605
XP_006710128                                                           15    9605
>NP_006715
          Length = 1558

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASRALGFAK 9
           AS+ALGFAK
Sbjct: 812 ASKALGFAK 820
>NP_005913
          Length = 1608

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASRALGFAK 9
           AS+ALGFAK
Sbjct: 812 ASKALGFAK 820
>XP_005267046
          Length = 1554

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASRALGFAK 9
           AS+ALGFAK
Sbjct: 812 ASKALGFAK 820
>XP_005267045
          Length = 1604

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASRALGFAK 9
           AS+ALGFAK
Sbjct: 812 ASKALGFAK 820
>NP_579878
          Length = 1365

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ASRALGFAK 9
           AS ALGF+K
Sbjct: 607 ASSALGFSK 615
>NP_015627
          Length = 629

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ASRALGFAK 9
           AS ALGF+K
Sbjct: 607 ASSALGFSK 615
>NP_579890
          Length = 1365

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ASRALGFAK 9
           AS ALGF+K
Sbjct: 607 ASSALGFSK 615
>NP_579889
          Length = 647

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ASRALGFAK 9
           AS ALGF+K
Sbjct: 607 ASSALGFSK 615
>NP_579877
          Length = 1365

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ASRALGFAK 9
           AS ALGF+K
Sbjct: 607 ASSALGFSK 615
>NP_001035889
          Length = 1365

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ASRALGFAK 9
           AS ALGF+K
Sbjct: 607 ASSALGFSK 615
>XP_006713977
          Length = 647

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ASRALGFAK 9
           AS ALGF+K
Sbjct: 607 ASSALGFSK 615
>XP_005248062
          Length = 647

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ASRALGFAK 9
           AS ALGF+K
Sbjct: 607 ASSALGFSK 615
>XP_005248058
          Length = 1365

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ASRALGFAK 9
           AS ALGF+K
Sbjct: 607 ASSALGFSK 615
>NP_001167555
          Length = 575

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           RALG+AK
Sbjct: 275 RALGYAK 281
>NP_037406
          Length = 575

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           RALG+AK
Sbjct: 275 RALGYAK 281
>XP_006717836
          Length = 406

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           RALG+AK
Sbjct: 106 RALGYAK 112
>XP_006717835
          Length = 406

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           RALG+AK
Sbjct: 106 RALGYAK 112
>XP_006717834
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           RALG+AK
Sbjct: 187 RALGYAK 193
>XP_006717833
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           RALG+AK
Sbjct: 187 RALGYAK 193
>NP_001186306
          Length = 720

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 434 QALGFAK 440
>NP_055977
          Length = 724

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 438 QALGFAK 444
>NP_001159777
          Length = 475

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 322 QALGFAK 328
>NP_056512
          Length = 421

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SRALGFAK 9
          SRA GFA+
Sbjct: 54 SRAAGFAR 61
>NP_001011655
          Length = 428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 275 QALGFAK 281
>NP_001159778
          Length = 396

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 275 QALGFAK 281
>NP_612408
          Length = 438

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 275 QALGFAK 281
>XP_006713887
          Length = 336

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 172 QALGFAK 178
>XP_006713886
          Length = 382

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 218 QALGFAK 224
>XP_005269429
          Length = 427

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 275 QALGFAK 281
>XP_005269428
          Length = 439

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALGFAK 9
           +ALGFAK
Sbjct: 275 QALGFAK 281
>NP_004068
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SRALGF 7
           SRALGF
Sbjct: 439 SRALGF 444
>NP_004057
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  ASRALGF 7
          A RALGF
Sbjct: 56 AMRALGF 62
>NP_689914
          Length = 5058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SRALGFAK 9
           SRAL F K
Sbjct: 706 SRALNFTK 713
>NP_004335
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  ASRALGF 7
          A RALGF
Sbjct: 56 AMRALGF 62
>XP_006726390
          Length = 1353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1191 ASRAAGF 1197
>XP_006726389
          Length = 1471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1309 ASRAAGF 1315
>XP_006726388
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1327 ASRAAGF 1333
>XP_006726387
          Length = 1497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1375 ASRAAGF 1381
>XP_006711142
          Length = 1471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1309 ASRAAGF 1315
>XP_006711141
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1327 ASRAAGF 1333
>XP_006711140
          Length = 1497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1375 ASRAAGF 1381
>XP_006710131
          Length = 1353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1191 ASRAAGF 1197
>XP_006710130
          Length = 1471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1309 ASRAAGF 1315
>XP_006710129
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1327 ASRAAGF 1333
>XP_006710128
          Length = 1497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    ASRALGF 7
            ASRA GF
Sbjct: 1375 ASRAAGF 1381
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.129    0.342 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 323,398
Number of extensions: 217
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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