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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1482
XP_005262424                                                           18    1482
NP_001275637                                                           17    3301
XP_005270355                                                           17    4312
XP_006718143                                                           17    4312
XP_005270354                                                           17    4312
XP_005270352                                                           17    4312
XP_005270351                                                           17    4312
XP_006718142                                                           17    4312
NP_542194                                                              16    5631
NP_001171864                                                           16    5631
NP_053586                                                              16    5631
NP_053584                                                              16    5631
NP_004874                                                              16    5631
NP_053585                                                              16    5631
NP_940873                                                              16    5631
NP_954650                                                              16    5631
XP_006722781                                                           16    5631
XP_005272524                                                           16    5631
XP_006714873                                                           16    5631
XP_006714872                                                           16    5631
XP_006714871                                                           16    5631
NP_001098714                                                           15    9605
NP_001269777                                                           15    9605
NP_001276876                                                           15    9605
NP_690608                                                              15    9605
NP_004674                                                              15    9605
XP_006722401                                                           15    9605
XP_006722400                                                           15    9605
XP_006722399                                                           15    9605
XP_006722398                                                           15    9605
NP_002836                                                              15    9605

>NP_722576
          Length = 3080

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASQTGQVSK 9
            ASQTG VSK
Sbjct: 1924 ASQTGLVSK 1932



>XP_005262424
          Length = 2875

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASQTGQVSK 9
            ASQTG VSK
Sbjct: 1719 ASQTGLVSK 1727



>NP_001275637
          Length = 158

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ASQTGQVSK 9
          ASQTG++ K
Sbjct: 6  ASQTGEIVK 14



>XP_005270355
          Length = 968

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SQTGQVSK 9
          S+TG+VSK
Sbjct: 70 SKTGEVSK 77



>XP_006718143
          Length = 999

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SQTGQVSK 9
           S+TG+VSK
Sbjct: 101 SKTGEVSK 108



>XP_005270354
          Length = 1014

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SQTGQVSK 9
           S+TG+VSK
Sbjct: 116 SKTGEVSK 123



>XP_005270352
          Length = 1118

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SQTGQVSK 9
           S+TG+VSK
Sbjct: 220 SKTGEVSK 227



>XP_005270351
          Length = 1133

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SQTGQVSK 9
           S+TG+VSK
Sbjct: 235 SKTGEVSK 242



>XP_006718142
          Length = 1164

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SQTGQVSK 9
           S+TG+VSK
Sbjct: 266 SKTGEVSK 273



>NP_542194
          Length = 1661

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   ASQTGQV 7
           A+QTGQV
Sbjct: 285 AAQTGQV 291



>NP_001171864
          Length = 784

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTGQVSK 9
           SQTGQV +
Sbjct: 244 SQTGQVGE 251



>NP_053586
          Length = 852

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTGQVSK 9
           SQTGQV +
Sbjct: 244 SQTGQVGE 251



>NP_053584
          Length = 844

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTGQVSK 9
           SQTGQV +
Sbjct: 244 SQTGQVGE 251



>NP_004874
          Length = 850

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTGQVSK 9
           SQTGQV +
Sbjct: 244 SQTGQVGE 251



>NP_053585
          Length = 858

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTGQVSK 9
           SQTGQV +
Sbjct: 244 SQTGQVGE 251



>NP_940873
          Length = 821

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ASQTGQVS 8
           ASQTGQ +
Sbjct: 692 ASQTGQTA 699



>NP_954650
          Length = 1016

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  ASQTGQV 7
          A+QTGQV
Sbjct: 47 AAQTGQV 53



>XP_006722781
          Length = 821

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ASQTGQVS 8
           ASQTGQ +
Sbjct: 692 ASQTGQTA 699



>XP_005272524
          Length = 872

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ASQTGQVS 8
           ASQTGQ +
Sbjct: 743 ASQTGQTA 750



>XP_006714873
          Length = 422

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTGQVSK 9
           SQTGQV +
Sbjct: 244 SQTGQVGE 251



>XP_006714872
          Length = 629

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQTGQVSK 9
           SQTGQV +
Sbjct: 244 SQTGQVGE 251



>XP_006714871
          Length = 647

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SQTGQVSK 9
          SQTGQV +
Sbjct: 41 SQTGQVGE 48



>NP_001098714
          Length = 1465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ASQTGQVSK 9
           ++Q+G+VSK
Sbjct: 533 SNQSGRVSK 541



>NP_001269777
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QTGQVS 8
           QTGQ+S
Sbjct: 130 QTGQIS 135



>NP_001276876
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QTGQVSK 9
          +TG+VSK
Sbjct: 8  ETGEVSK 14



>NP_690608
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QTGQVS 8
           QTGQ+S
Sbjct: 183 QTGQIS 188



>NP_004674
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QTGQVS 8
           QTGQ+S
Sbjct: 183 QTGQIS 188



>XP_006722401
          Length = 1432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ASQTGQVSK 9
           ++Q+G+VSK
Sbjct: 471 SNQSGRVSK 479



>XP_006722400
          Length = 1469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ASQTGQVSK 9
           ++Q+G+VSK
Sbjct: 533 SNQSGRVSK 541



>XP_006722399
          Length = 1477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ASQTGQVSK 9
           ++Q+G+VSK
Sbjct: 533 SNQSGRVSK 541



>XP_006722398
          Length = 1494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ASQTGQVSK 9
           ++Q+G+VSK
Sbjct: 533 SNQSGRVSK 541



>NP_002836
          Length = 1452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ASQTGQVSK 9
           ++Q+G+VSK
Sbjct: 533 SNQSGRVSK 541



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.287   0.0987    0.225 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 408,467
Number of extensions: 299
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)