Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              19     665
NP_005913                                                              19     665
XP_005267046                                                           19     665
XP_005267045                                                           19     665
NP_055244                                                              18    1935
XP_005247419                                                           18    1935
NP_001074319                                                           17    4312
NP_001852                                                              16    5631
NP_001006608                                                           16    5631
NP_955372                                                              16    5631
NP_055649                                                              16    5631
XP_005275724                                                           16    5631
XP_005275722                                                           16    5631
XP_005275721                                                           16    5631
XP_006725345                                                           16    5631
XP_006725344                                                           16    5631
XP_005275719                                                           16    5631
XP_006725343                                                           16    5631
XP_006725342                                                           16    5631
XP_005275717                                                           16    5631
XP_005275716                                                           16    5631
XP_005257941                                                           16    5631
XP_005257940                                                           16    5631
XP_006722274                                                           16    5631
XP_006722273                                                           16    5631
XP_005257939                                                           16    5631
XP_006722272                                                           16    5631
XP_005257937                                                           16    5631
XP_005257936                                                           16    5631
XP_005257935                                                           16    5631
XP_006722271                                                           16    5631
XP_005255855                                                           16    5631
NP_001032402                                                           16    5631
NP_056125                                                              16    7355
NP_055475                                                              16    7355
NP_004114                                                              16    7355
NP_002902                                                              16    7355
XP_005260072                                                           16    7355
XP_005273518                                                           16    7355
XP_006716381                                                           16    7355
XP_005249968                                                           16    7355
XP_005249967                                                           16    7355
XP_005249966                                                           16    7355
XP_006715864                                                           16    7355
XP_005249965                                                           16    7355
NP_057251                                                              15    9605
NP_001230469                                                           15    9605
NP_001137481                                                           15    9605
NP_001137482                                                           15    9605
NP_001137480                                                           15    9605
NP_001137475                                                           15    9605
NP_001137477                                                           15    9605
NP_001137478                                                           15    9605
NP_001137476                                                           15    9605
NP_115928                                                              15    9605
NP_001093143                                                           15    9605
NP_001093142                                                           15    9605
NP_001093141                                                           15    9605
NP_001093140                                                           15    9605
NP_004873                                                              15    9605
NP_112600                                                              15    9605
NP_064613                                                              15    9605
NP_001230470                                                           15    9605
XP_005262403                                                           15    9605
XP_005262402                                                           15    9605
XP_005262401                                                           15    9605
XP_005262400                                                           15    9605
XP_005262399                                                           15    9605
XP_005256979                                                           15    9605
XP_006721694                                                           15    9605
XP_005249230                                                           15    9605
NP_955469                                                              15    9605
NP_955471                                                              15    9605
NP_955470                                                              15    9605
>NP_006715
          Length = 1558

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASQSNLKEK 9
           AS+SNLKEK
Sbjct: 108 ASRSNLKEK 116
>NP_005913
          Length = 1608

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASQSNLKEK 9
           AS+SNLKEK
Sbjct: 108 ASRSNLKEK 116
>XP_005267046
          Length = 1554

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASQSNLKEK 9
           AS+SNLKEK
Sbjct: 108 ASRSNLKEK 116
>XP_005267045
          Length = 1604

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASQSNLKEK 9
           AS+SNLKEK
Sbjct: 108 ASRSNLKEK 116
>NP_055244
          Length = 984

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           A Q NLKEK
Sbjct: 334 AKQKNLKEK 342
>XP_005247419
          Length = 1014

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           A Q NLKEK
Sbjct: 334 AKQKNLKEK 342
>NP_001074319
          Length = 783

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SQSNLKEK 9
           SQ+NL+EK
Sbjct: 334 SQNNLQEK 341
>NP_001852
          Length = 169

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  ASQSNLKEK 9
          ASQ  LKEK
Sbjct: 57 ASQKALKEK 65
>NP_001006608
          Length = 1700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>NP_955372
          Length = 1634

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>NP_055649
          Length = 1700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005275724
          Length = 894

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005275722
          Length = 969

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005275721
          Length = 1018

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_006725345
          Length = 1600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_006725344
          Length = 1606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ASQSNLKEK 9
          ASQ +LK+K
Sbjct: 34 ASQQDLKDK 42
>XP_005275719
          Length = 1625

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_006725343
          Length = 1635

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_006725342
          Length = 1651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005275717
          Length = 1665

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005275716
          Length = 1676

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005257941
          Length = 969

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005257940
          Length = 1018

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_006722274
          Length = 1576

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_006722273
          Length = 1600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005257939
          Length = 1606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ASQSNLKEK 9
          ASQ +LK+K
Sbjct: 34 ASQQDLKDK 42
>XP_006722272
          Length = 1635

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005257937
          Length = 1651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005257936
          Length = 1665

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005257935
          Length = 1676

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_006722271
          Length = 1700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ +LK+K
Sbjct: 128 ASQQDLKDK 136
>XP_005255855
          Length = 169

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  ASQSNLKEK 9
          ASQ  LKEK
Sbjct: 57 ASQKALKEK 65
>NP_001032402
          Length = 211

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  QSNLKEK 9
          +SNLKEK
Sbjct: 47 KSNLKEK 53
>NP_056125
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SQSNLKEK 9
           SQS+LKE+
Sbjct: 119 SQSHLKEQ 126
>NP_055475
          Length = 888

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           A Q N+K+K
Sbjct: 638 AEQKNIKDK 646
>NP_004114
          Length = 153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ASQSNLKEK 9
           ASQ+N KE+
Sbjct: 102 ASQANRKEE 110
>NP_002902
          Length = 1118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ASQSNLKE 8
           ASQS+LK+
Sbjct: 214 ASQSSLKD 221
>XP_005260072
          Length = 1129

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ASQSNLKE 8
           ASQS+LK+
Sbjct: 214 ASQSSLKD 221
>XP_005273518
          Length = 732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SQSNLKEK 9
           SQS+LKE+
Sbjct: 119 SQSHLKEQ 126
>XP_006716381
          Length = 1014

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SQSNLKEK 9
          SQS+LKE+
Sbjct: 86 SQSHLKEQ 93
>XP_005249968
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           A Q N+K+K
Sbjct: 353 AEQKNIKDK 361
>XP_005249967
          Length = 892

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           A Q N+K+K
Sbjct: 638 AEQKNIKDK 646
>XP_005249966
          Length = 948

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           A Q N+K+K
Sbjct: 638 AEQKNIKDK 646
>XP_006715864
          Length = 957

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           A Q N+K+K
Sbjct: 638 AEQKNIKDK 646
>XP_005249965
          Length = 958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           A Q N+K+K
Sbjct: 638 AEQKNIKDK 646
>NP_057251
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ASQSNLKE 8
           ASQ+N+K+
Sbjct: 125 ASQTNIKK 132
>NP_001230469
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           ASQ  L+EK
Sbjct: 326 ASQCTLREK 334
>NP_001137481
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>NP_001137482
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>NP_001137480
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>NP_001137475
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>NP_001137477
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>NP_001137478
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>NP_001137476
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>NP_115928
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>NP_001093143
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  ASQSNLKEK 9
          ASQ N K K
Sbjct: 18 ASQKNFKAK 26
>NP_001093142
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  ASQSNLKEK 9
          ASQ N K K
Sbjct: 18 ASQKNFKAK 26
>NP_001093141
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  ASQSNLKEK 9
          ASQ N K K
Sbjct: 18 ASQKNFKAK 26
>NP_001093140
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  ASQSNLKEK 9
          ASQ N K K
Sbjct: 18 ASQKNFKAK 26
>NP_004873
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S+SN KEK
Sbjct: 280 SESNNKEK 287
>NP_112600
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ASQSNLKEK 9
          ASQSNL ++
Sbjct: 79 ASQSNLTKE 87
>NP_064613
          Length = 1160

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SQSNLKEK 9
            SQS L+EK
Sbjct: 1101 SQSELEEK 1108
>NP_001230470
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           ASQ  L+EK
Sbjct: 268 ASQCTLREK 276
>XP_005262403
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>XP_005262402
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>XP_005262401
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>XP_005262400
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>XP_005262399
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           S SNL+EK
Sbjct: 719 SYSNLEEK 726
>XP_005256979
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           ASQ  L+EK
Sbjct: 268 ASQCTLREK 276
>XP_006721694
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ASQSNLKEK 9
           ASQ  L+EK
Sbjct: 326 ASQCTLREK 334
>XP_005249230
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ASQSNLKE 8
           ASQ+N+K+
Sbjct: 125 ASQTNIKK 132
>NP_955469
          Length = 1156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SQSNLKEK 9
            SQS L+EK
Sbjct: 1097 SQSELEEK 1104
>NP_955471
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SQSNLKEK 9
           SQS L+EK
Sbjct: 964 SQSELEEK 971
>NP_955470
          Length = 1061

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SQSNLKEK 9
            SQS L+EK
Sbjct: 1002 SQSELEEK 1009
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.278   0.0958    0.206 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 498,971
Number of extensions: 606
Number of successful extensions: 74
Number of sequences better than 10000.0: 74
Number of HSP's gapped: 74
Number of HSP's successfully gapped: 74
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.6 bits)
S2: 28 (15.4 bits)

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