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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001273205                                                           16    7355
NP_001273203                                                           16    7355
NP_065982                                                              16    7355
NP_775922                                                              16    7355
NP_001273204                                                           16    7355
NP_001010893                                                           16    7355
NP_945314                                                              15    9605
NP_056502                                                              15    9605
NP_001276916                                                           15    9605
XP_005248772                                                           15    9605
XP_005248771                                                           15    9605
XP_005248770                                                           15    9605

>NP_001273205
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ASKIVSTV 8
           A KIVST+
Sbjct: 626 AKKIVSTI 633



>NP_001273203
          Length = 760

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ASKIVSTV 8
           A KIVST+
Sbjct: 666 AKKIVSTI 673



>NP_065982
          Length = 787

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ASKIVSTV 8
           A KIVST+
Sbjct: 693 AKKIVSTI 700



>NP_775922
          Length = 6996

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ASKIVSTVI 9
            A KIV+TV+
Sbjct: 2860 AGKIVNTVL 2868



>NP_001273204
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ASKIVSTV 8
           A KIVST+
Sbjct: 626 AKKIVSTI 633



>NP_001010893
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SKIVSTVI 9
           SKIVST++
Sbjct: 272 SKIVSTLL 279



>NP_945314
          Length = 1386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    SKIVSTVI 9
            S+I+ST++
Sbjct: 1373 SRIISTIV 1380



>NP_056502
          Length = 1213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ASKIVSTV 8
            ASK+ ST+
Sbjct: 1122 ASKVTSTI 1129



>NP_001276916
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ASKIVSTV 8
            ASK+ ST+
Sbjct: 1125 ASKVTSTI 1132



>XP_005248772
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ASKIVSTV 8
            ASK+ ST+
Sbjct: 1122 ASKVTSTI 1129



>XP_005248771
          Length = 1177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ASKIVSTV 8
            ASK+ ST+
Sbjct: 1122 ASKVTSTI 1129



>XP_005248770
          Length = 1213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ASKIVSTV 8
            ASK+ ST+
Sbjct: 1122 ASKVTSTI 1129



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.121    0.261 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 215,604
Number of extensions: 171
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)