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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              17    4312
NP_001035835                                                           15    9605
XP_006726483                                                           15    9605
XP_006714876                                                           15    9605

>NP_005350
          Length = 552

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ASAISLSAA 9
           A AISLSAA
Sbjct: 479 APAISLSAA 487



>NP_001035835
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  ASAISLSAA 9
          ASA+SLS++
Sbjct: 63 ASALSLSSS 71



>XP_006726483
          Length = 116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SAISLSAA 9
          +A+SLSAA
Sbjct: 37 AAVSLSAA 44



>XP_006714876
          Length = 116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SAISLSAA 9
          +A+SLSAA
Sbjct: 37 AAVSLSAA 44



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296   0.0972    0.194 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 191,320
Number of extensions: 125
Number of successful extensions: 4
Number of sequences better than 10000.0: 4
Number of HSP's gapped: 4
Number of HSP's successfully gapped: 4
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)