Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     869
NP_444254                                                              19     869
NP_444253                                                              19     869
NP_444255                                                              19     869
XP_005247551                                                           19     869
XP_005247550                                                           19     869
XP_006713712                                                           19     869
XP_005247549                                                           19     869
NP_056957                                                              17    4312
NP_001248396                                                           17    4312
NP_115517                                                              17    4312
XP_005265249                                                           17    4312
XP_006713247                                                           17    4312
XP_006713246                                                           17    4312
XP_005265248                                                           17    4312
NP_001138432                                                           16    5631
NP_001073280                                                           15    9605
NP_005210                                                              15    9605
NP_001003891                                                           15    9605
NP_056973                                                              15    9605
XP_005261689                                                           15    9605
XP_006724326                                                           15    9605
XP_005268441                                                           15    9605
>NP_444256
          Length = 1794

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASAFKQKLQ 9
            A AFKQKLQ
Sbjct: 1028 APAFKQKLQ 1036
>NP_444254
          Length = 1845

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASAFKQKLQ 9
            A AFKQKLQ
Sbjct: 1028 APAFKQKLQ 1036
>NP_444253
          Length = 1914

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASAFKQKLQ 9
            A AFKQKLQ
Sbjct: 1097 APAFKQKLQ 1105
>NP_444255
          Length = 1863

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASAFKQKLQ 9
            A AFKQKLQ
Sbjct: 1097 APAFKQKLQ 1105
>XP_005247551
          Length = 1855

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASAFKQKLQ 9
            A AFKQKLQ
Sbjct: 1038 APAFKQKLQ 1046
>XP_005247550
          Length = 1856

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASAFKQKLQ 9
            A AFKQKLQ
Sbjct: 1107 APAFKQKLQ 1115
>XP_006713712
          Length = 1923

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASAFKQKLQ 9
            A AFKQKLQ
Sbjct: 1107 APAFKQKLQ 1115
>XP_005247549
          Length = 1924

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ASAFKQKLQ 9
            A AFKQKLQ
Sbjct: 1107 APAFKQKLQ 1115
>NP_056957
          Length = 856

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ASAFKQKLQ 9
          A AF+Q+LQ
Sbjct: 76 AEAFQQRLQ 84
>NP_001248396
          Length = 504

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SAFKQKLQ 9
          SA+K+KLQ
Sbjct: 92 SAYKKKLQ 99
>NP_115517
          Length = 558

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SAFKQKLQ 9
           SA+K+KLQ
Sbjct: 146 SAYKKKLQ 153
>XP_005265249
          Length = 856

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ASAFKQKLQ 9
          A AF+Q+LQ
Sbjct: 76 AEAFQQRLQ 84
>XP_006713247
          Length = 856

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ASAFKQKLQ 9
          A AF+Q+LQ
Sbjct: 76 AEAFQQRLQ 84
>XP_006713246
          Length = 856

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ASAFKQKLQ 9
          A AF+Q+LQ
Sbjct: 76 AEAFQQRLQ 84
>XP_005265248
          Length = 856

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ASAFKQKLQ 9
          A AF+Q+LQ
Sbjct: 76 AEAFQQRLQ 84
>NP_001138432
          Length = 544

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   ASAFKQK 7
           ASAFK+K
Sbjct: 501 ASAFKEK 507
>NP_001073280
          Length = 1263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SAFKQKLQ 9
           S F+QKLQ
Sbjct: 498 SDFEQKLQ 505
>NP_005210
          Length = 1272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SAFKQKLQ 9
           S F+QKLQ
Sbjct: 507 SDFEQKLQ 514
>NP_001003891
          Length = 788

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  ASAFKQKL 8
          ++AF+QKL
Sbjct: 12 STAFRQKL 19
>NP_056973
          Length = 748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  ASAFKQKL 8
          ++AF+QKL
Sbjct: 12 STAFRQKL 19
>XP_005261689
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  ASAFKQKL 8
          ++AF+QKL
Sbjct: 12 STAFRQKL 19
>XP_006724326
          Length = 717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  ASAFKQKL 8
          ++AF+QKL
Sbjct: 12 STAFRQKL 19
>XP_005268441
          Length = 1250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SAFKQKLQ 9
           S F+QKLQ
Sbjct: 507 SDFEQKLQ 514
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.116    0.276 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 470,239
Number of extensions: 684
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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