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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001124320                                                           17    2528
NP_004901                                                              17    2528
NP_689728                                                              15    9605
NP_001243028                                                           15    9605
XP_006718871                                                           15    9605
NP_835228                                                              15    9605

>NP_001124320
          Length = 1243

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1    ARSVSLKL 8
            ARS+SLKL
Sbjct: 1232 ARSISLKL 1239



>NP_004901
          Length = 1244

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1    ARSVSLKL 8
            ARS+SLKL
Sbjct: 1233 ARSISLKL 1240



>NP_689728
          Length = 745

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  RSVSLKLR 9
          RS+S+KL+
Sbjct: 72 RSISIKLQ 79



>NP_001243028
          Length = 229

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  RSVSLKLR 9
          RS+++KLR
Sbjct: 6  RSITVKLR 13



>XP_006718871
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RSVSLKLR 9
           +SVS+K+R
Sbjct: 308 KSVSMKIR 315



>NP_835228
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RSVSLKLR 9
           +SVS+K+R
Sbjct: 373 KSVSMKIR 380



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.128    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 254,025
Number of extensions: 216
Number of successful extensions: 6
Number of sequences better than 10000.0: 6
Number of HSP's gapped: 6
Number of HSP's successfully gapped: 6
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)