Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000236                                                              20     390
NP_001120972                                                           20     390
XP_006716052                                                           20     390
XP_006716051                                                           20     390
NP_067018                                                              16    7355
NP_066192                                                              16    7355
NP_001248357                                                           15    9605
NP_663731                                                              15    9605
NP_001248356                                                           15    9605
NP_077320                                                              15    9605
NP_775110                                                              15    9605
NP_000061                                                              15    9605
NP_001167560                                                           15    9605
NP_114423                                                              15    9605
NP_001167561                                                           15    9605
NP_060681                                                              15    9605
NP_061950                                                              15    9605
NP_061949                                                              15    9605
NP_066307                                                              15    9605
XP_005260914                                                           15    9605
XP_005260913                                                           15    9605
XP_006722754                                                           15    9605
XP_005259877                                                           15    9605
XP_006711938                                                           15    9605
XP_006711937                                                           15    9605
XP_006711936                                                           15    9605
XP_006711935                                                           15    9605
XP_006711934                                                           15    9605
XP_006711933                                                           15    9605
XP_006711932                                                           15    9605
XP_006710801                                                           15    9605
NP_055790                                                              15    9605
>NP_000236
          Length = 1390

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ARQLGASLN 9
           ARQ+GASLN
Sbjct: 330 ARQIGASLN 338
>NP_001120972
          Length = 1408

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ARQLGASLN 9
           ARQ+GASLN
Sbjct: 330 ARQIGASLN 338
>XP_006716052
          Length = 1409

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ARQLGASLN 9
           ARQ+GASLN
Sbjct: 349 ARQIGASLN 357
>XP_006716051
          Length = 1427

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ARQLGASLN 9
           ARQ+GASLN
Sbjct: 349 ARQIGASLN 357
>NP_067018
          Length = 220

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RQLGASL 8
           R+LGASL
Sbjct: 158 RELGASL 164
>NP_066192
          Length = 217

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RQLGASL 8
           R+LGASL
Sbjct: 158 RELGASL 164
>NP_001248357
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 894 KQVGVSLN 901
>NP_663731
          Length = 890

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 809 KQVGVSLN 816
>NP_001248356
          Length = 896

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 815 KQVGVSLN 822
>NP_077320
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RQLGASL 8
           R+LGASL
Sbjct: 493 RKLGASL 499
>NP_775110
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RQLGASL 8
           R+LGASL
Sbjct: 445 RKLGASL 451
>NP_000061
          Length = 821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RQLGASL 8
           R+LGASL
Sbjct: 493 RKLGASL 499
>NP_001167560
          Length = 875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGASLN 9
           LGASLN
Sbjct: 524 LGASLN 529
>NP_114423
          Length = 891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGASLN 9
           LGASLN
Sbjct: 540 LGASLN 545
>NP_001167561
          Length = 918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGASLN 9
           LGASLN
Sbjct: 567 LGASLN 572
>NP_060681
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RQLGAS 7
           RQLGAS
Sbjct: 206 RQLGAS 211
>NP_061950
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QLGASLN 9
          QLG SLN
Sbjct: 65 QLGRSLN 71
>NP_061949
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QLGASLN 9
          QLG SLN
Sbjct: 65 QLGKSLN 71
>NP_066307
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QLGASLN 9
          QLG SLN
Sbjct: 65 QLGRSLN 71
>XP_005260914
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGASLN 9
           LGASLN
Sbjct: 505 LGASLN 510
>XP_005260913
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGASLN 9
           LGASLN
Sbjct: 647 LGASLN 652
>XP_006722754
          Length = 1169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ARQLGASLN 9
           A +LGASL+
Sbjct: 866 AEKLGASLS 874
>XP_005259877
          Length = 1394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ARQLGASLN 9
            A +LGASL+
Sbjct: 1091 AEKLGASLS 1099
>XP_006711938
          Length = 896

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 815 KQVGVSLN 822
>XP_006711937
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 845 KQVGVSLN 852
>XP_006711936
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 845 KQVGVSLN 852
>XP_006711935
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 845 KQVGVSLN 852
>XP_006711934
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 845 KQVGVSLN 852
>XP_006711933
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 851 KQVGVSLN 858
>XP_006711932
          Length = 939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQLGASLN 9
           +Q+G SLN
Sbjct: 858 KQVGVSLN 865
>XP_006710801
          Length = 647

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RQLGAS 7
           RQLGAS
Sbjct: 162 RQLGAS 167
>NP_055790
          Length = 1570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ARQLGASLN 9
            A +LGASL+
Sbjct: 1267 AEKLGASLS 1275
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.123    0.312 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 396,711
Number of extensions: 486
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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