Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              19     869
NP_001035957                                                           19     869
XP_006721991                                                           19     869
XP_006721990                                                           19     869
XP_006721989                                                           19     869
XP_005258041                                                           19     869
XP_006721988                                                           19     869
XP_006721987                                                           19     869
XP_006721986                                                           19     869
XP_005258040                                                           19     869
XP_006721985                                                           19     869
NP_005742                                                              16    5631
NP_671714                                                              16    5631
XP_006715890                                                           16    5631
XP_005250160                                                           16    5631
XP_005250159                                                           16    5631
NP_001277114                                                           16    7355
NP_068375                                                              16    7355
NP_001229831                                                           16    7355
NP_001229832                                                           16    7355
NP_003479                                                              16    7355
XP_006722190                                                           16    7355
XP_005257766                                                           16    7355
XP_005257764                                                           16    7355
XP_006722189                                                           16    7355
XP_005249641                                                           16    7355
NP_066939                                                              16    7355
NP_056246                                                              16    7355
>NP_000258
          Length = 2818

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 683 AQTKLEVAL 691
>NP_001035957
          Length = 2839

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 683 AQTKLEVAL 691
>XP_006721991
          Length = 1632

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 693 AQTKLEVAL 701
>XP_006721990
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 693 AQTKLEVAL 701
>XP_006721989
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 693 AQTKLEVAL 701
>XP_005258041
          Length = 2836

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 683 AQTKLEVAL 691
>XP_006721988
          Length = 2846

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 693 AQTKLEVAL 701
>XP_006721987
          Length = 2849

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 693 AQTKLEVAL 701
>XP_006721986
          Length = 2854

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 680 AQTKLEVAL 688
>XP_005258040
          Length = 2857

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 683 AQTKLEVAL 691
>XP_006721985
          Length = 2867

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   AQTKLEMAL 9
           AQTKLE+AL
Sbjct: 693 AQTKLEVAL 701
>NP_005742
          Length = 3907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    AQTKLEM 7
            AQTKLE+
Sbjct: 3169 AQTKLEL 3175
>NP_671714
          Length = 3899

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    AQTKLEM 7
            AQTKLE+
Sbjct: 3161 AQTKLEL 3167
>XP_006715890
          Length = 3860

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    AQTKLEM 7
            AQTKLE+
Sbjct: 3122 AQTKLEL 3128
>XP_005250160
          Length = 3939

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    AQTKLEM 7
            AQTKLE+
Sbjct: 3201 AQTKLEL 3207
>XP_005250159
          Length = 3952

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    AQTKLEM 7
            AQTKLE+
Sbjct: 3214 AQTKLEL 3220
>NP_001277114
          Length = 931

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QTKLEM 7
           QTKLEM
Sbjct: 348 QTKLEM 353
>NP_068375
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QTKLEM 7
           QTKLEM
Sbjct: 358 QTKLEM 363
>NP_001229831
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKLEMAL 9
           TKLE+AL
Sbjct: 129 TKLELAL 135
>NP_001229832
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKLEMAL 9
           TKLE+AL
Sbjct: 129 TKLELAL 135
>NP_003479
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKLEMAL 9
           TKLE+AL
Sbjct: 129 TKLELAL 135
>XP_006722190
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKLEMAL 9
           TKLE+AL
Sbjct: 129 TKLELAL 135
>XP_005257766
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKLEMAL 9
           TKLE+AL
Sbjct: 129 TKLELAL 135
>XP_005257764
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKLEMAL 9
           TKLE+AL
Sbjct: 129 TKLELAL 135
>XP_006722189
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TKLEMAL 9
           TKLE+AL
Sbjct: 129 TKLELAL 135
>XP_005249641
          Length = 979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   QTKLEMA 8
           QT+LEMA
Sbjct: 577 QTELEMA 583
>NP_066939
          Length = 1119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   QTKLEMA 8
           QT+LEMA
Sbjct: 577 QTELEMA 583
>NP_056246
          Length = 486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QTKLEMAL 9
           Q KL+MAL
Sbjct: 140 QVKLQMAL 147
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.117    0.265 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 313,342
Number of extensions: 363
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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