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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              18    1135
XP_005276064                                                           16    7355
NP_061184                                                              15    9605
NP_113609                                                              15    9605
XP_006722192                                                           15    9605

>NP_004351
          Length = 882

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AQSVEAGLQ 9
           AQ VEAGLQ
Sbjct: 698 AQPVEAGLQ 706



>XP_005276064
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AQSVEAG 7
           AQS+EAG
Sbjct: 291 AQSLEAG 297



>NP_061184
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QSVEAGLQ 9
           QS+E G+Q
Sbjct: 590 QSIEEGVQ 597



>NP_113609
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QSVEAG 7
           QSVEAG
Sbjct: 567 QSVEAG 572



>XP_006722192
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   QSVEAG 7
           QSVEAG
Sbjct: 404 QSVEAG 409



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.113    0.262 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 319,436
Number of extensions: 168
Number of successful extensions: 5
Number of sequences better than 10000.0: 5
Number of HSP's gapped: 5
Number of HSP's successfully gapped: 5
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)