Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           19     665
NP_004561                                                              19     665
NP_001275703                                                           19     665
XP_005253450                                                           19     665
XP_006719151                                                           19     665
NP_076918                                                              16    5631
NP_008831                                                              16    5631
NP_001188357                                                           15    9605
NP_056345                                                              15    9605
NP_077299                                                              15    9605
XP_005260135                                                           15    9605
XP_006722948                                                           15    9605
XP_006712471                                                           15    9605
>NP_001275701
          Length = 1486

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AQMSAINPA 9
            AQMSAI+PA
Sbjct: 1218 AQMSAISPA 1226
>NP_004561
          Length = 1445

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AQMSAINPA 9
            AQMSAI+PA
Sbjct: 1177 AQMSAISPA 1185
>NP_001275703
          Length = 1264

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AQMSAINPA 9
            AQMSAI+PA
Sbjct: 996  AQMSAISPA 1004
>XP_005253450
          Length = 1486

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AQMSAINPA 9
            AQMSAI+PA
Sbjct: 1218 AQMSAISPA 1226
>XP_006719151
          Length = 1486

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AQMSAINPA 9
            AQMSAI+PA
Sbjct: 1218 AQMSAISPA 1226
>NP_076918
          Length = 189

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AQMSAINPA 9
          AQMS I+P+
Sbjct: 42 AQMSRISPS 50
>NP_008831
          Length = 190

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AQMSAINPA 9
          AQMS I+P+
Sbjct: 43 AQMSRISPS 51
>NP_001188357
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   QMSAINP 8
           Q+S++NP
Sbjct: 167 QLSSVNP 173
>NP_056345
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   QMSAINP 8
           Q+S++NP
Sbjct: 235 QLSSVNP 241
>NP_077299
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QMSAINPA 9
           Q SAI+PA
Sbjct: 362 QASAISPA 369
>XP_005260135
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QMSAINPA 9
           Q SAI+PA
Sbjct: 244 QASAISPA 251
>XP_006722948
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QMSAINPA 9
           Q SAI+PA
Sbjct: 362 QASAISPA 369
>XP_006712471
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   QMSAINP 8
           Q+S++NP
Sbjct: 167 QLSSVNP 173
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.109    0.283 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 456,123
Number of extensions: 400
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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