Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_062541                                                              18    1482
NP_006268                                                              18    1482
NP_055833                                                              17    2528
NP_001186871                                                           17    2528
XP_006715463                                                           17    2528
XP_006715462                                                           17    2528
XP_006715461                                                           17    2528
XP_006715460                                                           17    2528
XP_006715459                                                           17    2528
XP_006715458                                                           17    2528
XP_006715457                                                           17    2528
XP_006715456                                                           17    2528
NP_060606                                                              17    3301
NP_057033                                                              17    4312
NP_001026789                                                           16    7355
NP_778234                                                              16    7355
NP_001159811                                                           16    7355
XP_006716168                                                           16    7355
XP_006716061                                                           16    7355
XP_006716060                                                           16    7355
XP_005250150                                                           16    7355
XP_006715884                                                           16    7355
XP_005249776                                                           16    7355
XP_005249775                                                           16    7355
NP_001139682                                                           16    7355
NP_001277622                                                           15    9605
NP_004381                                                              15    9605
NP_115640                                                              15    9605
NP_001269609                                                           15    9605
NP_001269608                                                           15    9605
NP_001269610                                                           15    9605
NP_001243345                                                           15    9605
NP_001243346                                                           15    9605
NP_055847                                                              15    9605
NP_076996                                                              15    9605
NP_055592                                                              15    9605
NP_056175                                                              15    9605
NP_689838                                                              15    9605
NP_001193856                                                           15    9605
XP_005254238                                                           15    9605
XP_005266355                                                           15    9605
XP_005266354                                                           15    9605
XP_005266353                                                           15    9605
XP_005266352                                                           15    9605
XP_005251961                                                           15    9605
XP_006717107                                                           15    9605
XP_006717106                                                           15    9605
XP_006717105                                                           15    9605
XP_005251958                                                           15    9605
XP_006715067                                                           15    9605
XP_006714561                                                           15    9605
XP_005273417                                                           15    9605
XP_006711022                                                           15    9605
XP_006711021                                                           15    9605
XP_006711020                                                           15    9605
>NP_062541
          Length = 1670

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    AQFQMYRT 8
            AQF+MY+T
Sbjct: 1457 AQFKMYKT 1464
>NP_006268
          Length = 1697

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    AQFQMYRT 8
            AQF+MY+T
Sbjct: 1484 AQFKMYKT 1491
>NP_055833
          Length = 2465

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2318 QFQMYR 2323
>NP_001186871
          Length = 2476

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2309 QFQMYR 2314
>XP_006715463
          Length = 2434

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2267 QFQMYR 2272
>XP_006715462
          Length = 2452

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2285 QFQMYR 2290
>XP_006715461
          Length = 2456

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2309 QFQMYR 2314
>XP_006715460
          Length = 2465

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2318 QFQMYR 2323
>XP_006715459
          Length = 2475

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2308 QFQMYR 2313
>XP_006715458
          Length = 2476

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2309 QFQMYR 2314
>XP_006715457
          Length = 2485

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2318 QFQMYR 2323
>XP_006715456
          Length = 2485

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QFQMYR 7
            QFQMYR
Sbjct: 2318 QFQMYR 2323
>NP_060606
          Length = 3477

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AQFQMYRT 8
            A F+MYRT
Sbjct: 2489 ATFRMYRT 2496
>NP_057033
          Length = 327

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  QMYRTL 9
          QMYRTL
Sbjct: 44 QMYRTL 49
>NP_001026789
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 338 RFQLYRSM 345
>NP_778234
          Length = 336

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 247 RFQLYRSM 254
>NP_001159811
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 338 RFQLYRSM 345
>XP_006716168
          Length = 403

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 338 RFQLYRSM 345
>XP_006716061
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 338 RFQLYRSM 345
>XP_006716060
          Length = 403

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 338 RFQLYRSM 345
>XP_005250150
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 338 RFQLYRSM 345
>XP_006715884
          Length = 362

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 298 RFQLYRSM 305
>XP_005249776
          Length = 376

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 287 RFQLYRSM 294
>XP_005249775
          Length = 377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 287 RFQLYRSM 294
>NP_001139682
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   QFQMYRTL 9
           +FQ+YR++
Sbjct: 338 RFQLYRSM 345
>NP_001277622
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   QFQMYRTL 9
           QFQ YR +
Sbjct: 306 QFQRYRLI 313
>NP_004381
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   FQMYRT 8
           F MYRT
Sbjct: 259 FMMYRT 264
>NP_115640
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   AQFQMYRT 8
           A FQ YR+
Sbjct: 586 ASFQQYRS 593
>NP_001269609
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           QMY+TL
Sbjct: 286 QMYKTL 291
>NP_001269608
          Length = 1478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           QMY+TL
Sbjct: 286 QMYKTL 291
>NP_001269610
          Length = 1439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           QMY+TL
Sbjct: 286 QMYKTL 291
>NP_001243345
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   AQFQMYRT 8
           A FQ YR+
Sbjct: 574 ASFQQYRS 581
>NP_001243346
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   AQFQMYRT 8
           A FQ YR+
Sbjct: 516 ASFQQYRS 523
>NP_055847
          Length = 1447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           Q+YRTL
Sbjct: 156 QLYRTL 161
>NP_076996
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  AQFQMYRTL 9
          + FQM+R L
Sbjct: 14 SDFQMHRAL 22
>NP_055592
          Length = 1524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   AQFQMYR 7
           AQF+ YR
Sbjct: 385 AQFRFYR 391
>NP_056175
          Length = 1042

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   QFQMYRTL 9
           QFQ YR +
Sbjct: 591 QFQHYRAI 598
>NP_689838
          Length = 744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FQMYRTL 9
           FQ YR+L
Sbjct: 136 FQFYRSL 142
>NP_001193856
          Length = 4707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FQMYRTL 9
            FQ Y+TL
Sbjct: 1224 FQKYKTL 1230
>XP_005254238
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   FQMYRT 8
           F MYRT
Sbjct: 221 FMMYRT 226
>XP_005266355
          Length = 1448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           Q+YRTL
Sbjct: 156 QLYRTL 161
>XP_005266354
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           Q+YRTL
Sbjct: 156 QLYRTL 161
>XP_005266353
          Length = 1445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           Q+YRTL
Sbjct: 156 QLYRTL 161
>XP_005266352
          Length = 1446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           Q+YRTL
Sbjct: 156 QLYRTL 161
>XP_005251961
          Length = 1433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           QMY+TL
Sbjct: 286 QMYKTL 291
>XP_006717107
          Length = 1439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           QMY+TL
Sbjct: 286 QMYKTL 291
>XP_006717106
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           QMY+TL
Sbjct: 286 QMYKTL 291
>XP_006717105
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           QMY+TL
Sbjct: 286 QMYKTL 291
>XP_005251958
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QMYRTL 9
           QMY+TL
Sbjct: 286 QMYKTL 291
>XP_006715067
          Length = 4707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FQMYRTL 9
            FQ Y+TL
Sbjct: 1224 FQKYKTL 1230
>XP_006714561
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  AQFQMYRTL 9
          + FQM+R L
Sbjct: 14 SDFQMHRAL 22
>XP_005273417
          Length = 1487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   AQFQMYR 7
           AQF+ YR
Sbjct: 385 AQFRFYR 391
>XP_006711022
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   AQFQMYRT 8
           A FQ YR+
Sbjct: 530 ASFQQYRS 537
>XP_006711021
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   AQFQMYRT 8
           A FQ YR+
Sbjct: 530 ASFQQYRS 537
>XP_006711020
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   AQFQMYRT 8
           A FQ YR+
Sbjct: 549 ASFQQYRS 556
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 424,407
Number of extensions: 634
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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