Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245202                                                           20     390
NP_001245200                                                           20     390
NP_001161091                                                           20     390
NP_000240                                                              20     390
NP_001161090                                                           20     390
NP_001161089                                                           20     390
NP_001245203                                                           20     390
XP_005265223                                                           20     390
XP_005265221                                                           20     390
XP_005265220                                                           20     390
XP_005265218                                                           20     390
NP_000312                                                              17    4312
NP_002702                                                              17    4312
NP_001018125                                                           16    5631
NP_149075                                                              16    5631
NP_036220                                                              16    5631
XP_005277590                                                           16    5631
XP_006713088                                                           16    5631
XP_006713087                                                           16    5631
XP_005265003                                                           16    5631
XP_006713086                                                           16    5631
XP_006713085                                                           16    5631
XP_006713084                                                           16    5631
NP_001231612                                                           16    7355
NP_000912                                                              16    7355
NP_775822                                                              16    7355
NP_055417                                                              16    7355
XP_006719150                                                           16    7355
XP_006719149                                                           16    7355
XP_006717062                                                           16    7355
XP_006717061                                                           16    7355
XP_005251851                                                           16    7355
XP_005251850                                                           16    7355
NP_060726                                                              15    9605
NP_699179                                                              15    9605
NP_001276903                                                           15    9605
XP_003960167                                                           15    9605
XP_005253925                                                           15    9605
XP_005253924                                                           15    9605
XP_005253923                                                           15    9605
XP_003846269                                                           15    9605
XP_003846670                                                           15    9605
NP_060797                                                              15    9605
NP_036306                                                              15    9605
>NP_001245202
          Length = 515

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 339 APLFDLAML 347
>NP_001245200
          Length = 687

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 580 APLFDLAML 588
>NP_001161091
          Length = 515

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 339 APLFDLAML 347
>NP_000240
          Length = 756

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 580 APLFDLAML 588
>NP_001161090
          Length = 515

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 339 APLFDLAML 347
>NP_001161089
          Length = 658

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 482 APLFDLAML 490
>NP_001245203
          Length = 515

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 339 APLFDLAML 347
>XP_005265223
          Length = 415

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 239 APLFDLAML 247
>XP_005265221
          Length = 515

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 339 APLFDLAML 347
>XP_005265220
          Length = 515

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 339 APLFDLAML 347
>XP_005265218
          Length = 687

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLFDLVML 9
           APLFDL ML
Sbjct: 511 APLFDLAML 519
>NP_000312
          Length = 928

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   APLFDLV 7
           +PLFDL+
Sbjct: 567 SPLFDLI 573
>NP_002702
          Length = 1122

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   APLFDL 6
           APLFDL
Sbjct: 107 APLFDL 112
>NP_001018125
          Length = 1451

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LFDLVML 9
           +FDLVML
Sbjct: 379 VFDLVML 385
>NP_149075
          Length = 1471

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LFDLVML 9
           +FDLVML
Sbjct: 379 VFDLVML 385
>NP_036220
          Length = 258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   PLFDLVML 9
           P+FDL++L
Sbjct: 140 PVFDLLIL 147
>XP_005277590
          Length = 900

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LFDLVML 9
           +FDLVML
Sbjct: 126 VFDLVML 132
>XP_006713088
          Length = 1382

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LFDLVML 9
           +FDLVML
Sbjct: 379 VFDLVML 385
>XP_006713087
          Length = 1432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LFDLVML 9
           +FDLVML
Sbjct: 379 VFDLVML 385
>XP_005265003
          Length = 1451

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LFDLVML 9
           +FDLVML
Sbjct: 379 VFDLVML 385
>XP_006713086
          Length = 1458

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LFDLVML 9
           +FDLVML
Sbjct: 379 VFDLVML 385
>XP_006713085
          Length = 1471

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LFDLVML 9
           +FDLVML
Sbjct: 379 VFDLVML 385
>XP_006713084
          Length = 1471

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LFDLVML 9
           +FDLVML
Sbjct: 379 VFDLVML 385
>NP_001231612
          Length = 819

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PLFDLV 7
           P+FDLV
Sbjct: 373 PIFDLV 378
>NP_000912
          Length = 1141

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PLFDLV 7
           P+FDLV
Sbjct: 695 PIFDLV 700
>NP_775822
          Length = 871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FDLVML 9
           FDLVML
Sbjct: 397 FDLVML 402
>NP_055417
          Length = 1358

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   PLFDLVML 9
           PLFDL  L
Sbjct: 257 PLFDLSQL 264
>XP_006719150
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PLFDLV 7
           P+FDLV
Sbjct: 377 PIFDLV 382
>XP_006719149
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PLFDLV 7
           P+FDLV
Sbjct: 695 PIFDLV 700
>XP_006717062
          Length = 828

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FDLVML 9
           FDLVML
Sbjct: 339 FDLVML 344
>XP_006717061
          Length = 872

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FDLVML 9
           FDLVML
Sbjct: 397 FDLVML 402
>XP_005251851
          Length = 885

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FDLVML 9
           FDLVML
Sbjct: 397 FDLVML 402
>XP_005251850
          Length = 886

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FDLVML 9
           FDLVML
Sbjct: 397 FDLVML 402
>NP_060726
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   APLFDL 6
           APL+DL
Sbjct: 377 APLYDL 382
>NP_699179
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   APLFDLVML 9
           A  FD+VML
Sbjct: 317 ASAFDVVML 325
>NP_001276903
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   APLFDL 6
           APL+DL
Sbjct: 317 APLYDL 322
>XP_003960167
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   APLFDL 6
           APL+DL
Sbjct: 400 APLYDL 405
>XP_005253925
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   APLFDLVML 9
           A  FD+VML
Sbjct: 136 ASAFDVVML 144
>XP_005253924
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   APLFDLVML 9
           A  FD+VML
Sbjct: 136 ASAFDVVML 144
>XP_005253923
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   APLFDLVML 9
           A  FD+VML
Sbjct: 317 ASAFDVVML 325
>XP_003846269
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   APLFDL 6
           APL+DL
Sbjct: 400 APLYDL 405
>XP_003846670
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   APLFDL 6
           APL+DL
Sbjct: 407 APLYDL 412
>NP_060797
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PLFDLV 7
           PLFDL+
Sbjct: 136 PLFDLM 141
>NP_036306
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   APLFDLVML 9
           A  FD+VML
Sbjct: 251 ASAFDVVML 259
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.158    0.470 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 443,876
Number of extensions: 549
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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