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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              18    1135
NP_001073936                                                           17    2528
NP_872577                                                              17    4312
NP_000186                                                              17    4312
NP_690047                                                              17    4312
XP_006717881                                                           17    4312
XP_005269815                                                           17    4312
XP_005269813                                                           17    4312
XP_005269814                                                           17    4312
XP_005269812                                                           17    4312
NP_001129206                                                           16    5631
NP_597733                                                              16    5631
XP_006719031                                                           16    5631
XP_006719030                                                           16    5631
XP_006719029                                                           16    5631
XP_006719028                                                           16    5631
XP_006719027                                                           16    5631
XP_006719026                                                           16    5631
NP_000287                                                              16    7355
NP_001009944                                                           16    7355
XP_006720958                                                           16    7355
XP_006720957                                                           16    7355
XP_005255427                                                           16    7355
XP_006720956                                                           16    7355
XP_006720955                                                           16    7355
XP_006720954                                                           16    7355
XP_006720953                                                           16    7355
XP_006720952                                                           16    7355
NP_659425                                                              15    9605
NP_054706                                                              15    9605
NP_003364                                                              15    9605
NP_075053                                                              15    9605
XP_005270200                                                           15    9605
XP_005270199                                                           15    9605
XP_006717808                                                           15    9605
XP_006714735                                                           15    9605
XP_005248626                                                           15    9605

>NP_073713
          Length = 985

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APIIVSAML 9
           APIIVS ML
Sbjct: 547 APIIVSTML 555



>NP_001073936
          Length = 1848

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2    PIIVSAML 9
            P+IVSAML
Sbjct: 1615 PMIVSAML 1622



>NP_872577
          Length = 324

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  APIIVSAM 8
          AP+I+S+M
Sbjct: 52 APVIISSM 59



>NP_000186
          Length = 700

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  APIIVSAM 8
          AP+I+S+M
Sbjct: 52 APVIISSM 59



>NP_690047
          Length = 140

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   APIIVSAML 9
           AP++V+AM+
Sbjct: 99  APLLVNAMI 107



>XP_006717881
          Length = 613

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  APIIVSAM 8
          AP+I+S+M
Sbjct: 52 APVIISSM 59



>XP_005269815
          Length = 667

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  APIIVSAM 8
          AP+I+S+M
Sbjct: 52 APVIISSM 59



>XP_005269813
          Length = 700

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  APIIVSAM 8
          AP+I+S+M
Sbjct: 52 APVIISSM 59



>XP_005269814
          Length = 700

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  APIIVSAM 8
          AP+I+S+M
Sbjct: 52 APVIISSM 59



>XP_005269812
          Length = 700

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  APIIVSAM 8
          AP+I+S+M
Sbjct: 52 APVIISSM 59



>NP_001129206
          Length = 577

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   APIIVSAM 8
           AP+IVSA+
Sbjct: 144 APMIVSAL 151



>NP_597733
          Length = 516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   APIIVSAM 8
           AP+IVSA+
Sbjct: 144 APMIVSAL 151



>XP_006719031
          Length = 561

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   APIIVSAM 8
           AP+IVSA+
Sbjct: 128 APMIVSAL 135



>XP_006719030
          Length = 561

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   APIIVSAM 8
           AP+IVSA+
Sbjct: 128 APMIVSAL 135



>XP_006719029
          Length = 577

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   APIIVSAM 8
           AP+IVSA+
Sbjct: 144 APMIVSAL 151



>XP_006719028
          Length = 577

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   APIIVSAM 8
           AP+IVSA+
Sbjct: 144 APMIVSAL 151



>XP_006719027
          Length = 577

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   APIIVSAM 8
           AP+IVSA+
Sbjct: 144 APMIVSAL 151



>XP_006719026
          Length = 577

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   APIIVSAM 8
           AP+IVSA+
Sbjct: 144 APMIVSAL 151



>NP_000287
          Length = 4302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    APIIVSA 7
            AP++VSA
Sbjct: 1227 APVVVSA 1233



>NP_001009944
          Length = 4303

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    APIIVSA 7
            AP++VSA
Sbjct: 1227 APVVVSA 1233



>XP_006720958
          Length = 2675

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    APIIVSA 7
            AP++VSA
Sbjct: 1264 APVVVSA 1270



>XP_006720957
          Length = 2688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    APIIVSA 7
            AP++VSA
Sbjct: 1264 APVVVSA 1270



>XP_005255427
          Length = 3288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   APIIVSA 7
           AP++VSA
Sbjct: 212 APVVVSA 218



>XP_006720956
          Length = 3895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   APIIVSA 7
           AP++VSA
Sbjct: 819 APVVVSA 825



>XP_006720955
          Length = 4279

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    APIIVSA 7
            AP++VSA
Sbjct: 1203 APVVVSA 1209



>XP_006720954
          Length = 4334

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    APIIVSA 7
            AP++VSA
Sbjct: 1264 APVVVSA 1270



>XP_006720953
          Length = 4339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    APIIVSA 7
            AP++VSA
Sbjct: 1264 APVVVSA 1270



>XP_006720952
          Length = 4340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    APIIVSA 7
            AP++VSA
Sbjct: 1264 APVVVSA 1270



>NP_659425
          Length = 216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PIIVSAML 9
           PI VSA+L
Sbjct: 151 PICVSALL 158



>NP_054706
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   PIIVSAM 8
           P+++SAM
Sbjct: 203 PVLISAM 209



>NP_003364
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   PIIVSAM 8
           P+++SAM
Sbjct: 203 PVLISAM 209



>NP_075053
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PIIVSAML 9
           PI +SA+L
Sbjct: 308 PIFISALL 315



>XP_005270200
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   PIIVSAM 8
           P+++SAM
Sbjct: 203 PVLISAM 209



>XP_005270199
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   PIIVSAM 8
           P+++SAM
Sbjct: 203 PVLISAM 209



>XP_006717808
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  APIIVSA 7
          AP++VSA
Sbjct: 19 APVLVSA 25



>XP_006714735
          Length = 722

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PIIVSAML 9
           PI +SA+L
Sbjct: 308 PIFISALL 315



>XP_005248626
          Length = 724

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PIIVSAML 9
           PI +SA+L
Sbjct: 267 PIFISALL 274



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.138    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 301,032
Number of extensions: 339
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)