Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           18    1135
NP_036462                                                              18    1135
NP_001243397                                                           18    1135
XP_005269721                                                           18    1135
NP_036418                                                              17    2528
NP_001135891                                                           17    2528
NP_001135897                                                           17    2528
NP_001135893                                                           17    2528
NP_001135898                                                           17    2528
NP_001135895                                                           17    2528
NP_001135892                                                           17    2528
NP_001135894                                                           17    2528
NP_001135904                                                           17    2528
NP_001135903                                                           17    2528
NP_001135902                                                           17    2528
NP_001135901                                                           17    2528
NP_001135899                                                           17    2528
NP_001135890                                                           17    2528
NP_001135896                                                           17    2528
NP_001135900                                                           17    2528
NP_001127911                                                           17    4312
NP_001127910                                                           17    4312
NP_665696                                                              17    4312
NP_001127909                                                           17    4312
NP_277052                                                              16    5631
NP_056224                                                              16    5631
NP_003912                                                              16    7355
NP_004230                                                              16    7355
XP_006726441                                                           16    7355
XP_005268271                                                           16    7355
XP_005268270                                                           16    7355
XP_006720384                                                           16    7355
XP_006711918                                                           16    7355
XP_005271368                                                           16    7355
XP_003403763                                                           16    7355
NP_110399                                                              15    9605
NP_001230072                                                           15    9605
NP_057443                                                              15    9605
NP_060924                                                              15    9605
NP_001059                                                              15    9605
NP_001230071                                                           15    9605
NP_001193620                                                           15    9605
NP_998759                                                              15    9605
XP_005254487                                                           15    9605
XP_006720624                                                           15    9605
XP_005254486                                                           15    9605
XP_006720623                                                           15    9605
XP_005254485                                                           15    9605
XP_006720622                                                           15    9605
XP_005265484                                                           15    9605
NP_115880                                                              15    9605
>NP_001243398
          Length = 1781

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ANSASLFTP 9
            ANSASL TP
Sbjct: 1552 ANSASLSTP 1560
>NP_036462
          Length = 2073

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ANSASLFTP 9
            ANSASL TP
Sbjct: 1844 ANSASLSTP 1852
>NP_001243397
          Length = 1890

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ANSASLFTP 9
            ANSASL TP
Sbjct: 1661 ANSASLSTP 1669
>XP_005269721
          Length = 2073

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ANSASLFTP 9
            ANSASL TP
Sbjct: 1844 ANSASLSTP 1852
>NP_036418
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135891
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135897
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135893
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135898
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135895
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135892
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135894
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135904
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135903
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135902
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135901
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135899
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135890
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135896
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135900
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   NSASLFT 8
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001127911
          Length = 1253

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           +SAS FTP
Sbjct: 528 SSASFFTP 535
>NP_001127910
          Length = 1253

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           +SAS FTP
Sbjct: 528 SSASFFTP 535
>NP_665696
          Length = 1210

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           +SAS FTP
Sbjct: 528 SSASFFTP 535
>NP_001127909
          Length = 1237

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           +SAS FTP
Sbjct: 555 SSASFFTP 562
>NP_277052
          Length = 1731

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ANSASLFTP 9
           A SASLF P
Sbjct: 936 AFSASLFAP 944
>NP_056224
          Length = 3123

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           N+ASL TP
Sbjct: 546 NAASLHTP 553
>NP_003912
          Length = 233

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ANSASLFTP 9
           ANS+ +F P
Sbjct: 216 ANSSEMFLP 224
>NP_004230
          Length = 1979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NSASLFTP 9
            +SASL TP
Sbjct: 1317 SSASLLTP 1324
>XP_006726441
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASLFTP 9
           SASL+TP
Sbjct: 130 SASLWTP 136
>XP_005268271
          Length = 1537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           +SASL TP
Sbjct: 875 SSASLLTP 882
>XP_005268270
          Length = 1950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NSASLFTP 9
            +SASL TP
Sbjct: 1288 SSASLLTP 1295
>XP_006720384
          Length = 1978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    NSASLFTP 9
            +SASL TP
Sbjct: 1316 SSASLLTP 1323
>XP_006711918
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASLFTP 9
           SASL+TP
Sbjct: 130 SASLWTP 136
>XP_005271368
          Length = 222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ANSASLFTP 9
           ANS+ +F P
Sbjct: 205 ANSSEMFLP 213
>XP_003403763
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASLFTP 9
           SASL+TP
Sbjct: 130 SASLWTP 136
>NP_110399
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NS+S+F P
Sbjct: 301 NSSSVFQP 308
>NP_001230072
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 317 NSAAVITP 324
>NP_057443
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 332 NSAAVITP 339
>NP_060924
          Length = 440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 331 NSAAVITP 338
>NP_001059
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NSASLFTP 9
            +SAS+F+P
Sbjct: 1465 DSASVFSP 1472
>NP_001230071
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 330 NSAAVITP 337
>NP_001193620
          Length = 744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NS+ ++TP
Sbjct: 706 NSSDMYTP 713
>NP_998759
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NS+ ++TP
Sbjct: 697 NSSDMYTP 704
>XP_005254487
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 316 NSAAVITP 323
>XP_006720624
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 323 NSAAVITP 330
>XP_005254486
          Length = 440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 331 NSAAVITP 338
>XP_006720623
          Length = 446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 337 NSAAVITP 344
>XP_005254485
          Length = 446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 337 NSAAVITP 344
>XP_006720622
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NSA++ TP
Sbjct: 338 NSAAVITP 345
>XP_005265484
          Length = 1626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NSASLFTP 9
            +SAS+F+P
Sbjct: 1470 DSASVFSP 1477
>NP_115880
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NSASLFTP 9
           NS+ ++TP
Sbjct: 603 NSSDMYTP 610
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.114    0.309 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 375,457
Number of extensions: 478
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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