Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     665
NP_001075109                                                           19     665
NP_597677                                                              17    3301
NP_056199                                                              17    3301
XP_005248339                                                           17    3301
XP_005248337                                                           17    3301
XP_006714531                                                           17    3301
XP_006714530                                                           17    3301
XP_005248180                                                           17    3301
XP_006714090                                                           17    3301
NP_003206                                                              17    3301
NP_115599                                                              16    5631
XP_006718613                                                           16    5631
XP_006718612                                                           16    5631
XP_005274044                                                           16    5631
XP_005274045                                                           16    5631
NP_001127907                                                           16    7355
NP_115639                                                              16    7355
XP_006713848                                                           16    7355
XP_006713847                                                           16    7355
NP_055826                                                              15    9605
NP_001185879                                                           15    9605
NP_003464                                                              15    9605
NP_277050                                                              15    9605
XP_005252660                                                           15    9605
XP_005267270                                                           15    9605
XP_005267269                                                           15    9605

>NP_008835
          Length = 4128

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AMYDIIAAE 9
            AM+DIIAAE
Sbjct: 1302 AMHDIIAAE 1310



>NP_001075109
          Length = 4097

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    AMYDIIAAE 9
            AM+DIIAAE
Sbjct: 1302 AMHDIIAAE 1310



>NP_597677
          Length = 2804

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AMYDIIAA 8
           AMYDII++
Sbjct: 343 AMYDIISS 350



>NP_056199
          Length = 2697

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AMYDIIAA 8
           AMYDII++
Sbjct: 343 AMYDIISS 350



>XP_005248339
          Length = 2584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AMYDIIAA 8
           AMYDII++
Sbjct: 123 AMYDIISS 130



>XP_005248337
          Length = 2698

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AMYDIIAA 8
           AMYDII++
Sbjct: 343 AMYDIISS 350



>XP_006714531
          Length = 2738

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AMYDIIAA 8
           AMYDII++
Sbjct: 343 AMYDIISS 350



>XP_006714530
          Length = 2755

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   AMYDIIAA 8
           AMYDII++
Sbjct: 343 AMYDIISS 350



>XP_005248180
          Length = 551

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   AMYDIIAAE 9
           AMYD  AAE
Sbjct: 106 AMYDFQAAE 114



>XP_006714090
          Length = 551

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   AMYDIIAAE 9
           AMYD  AAE
Sbjct: 106 AMYDFQAAE 114



>NP_003206
          Length = 631

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   AMYDIIAAE 9
           AMYD  AAE
Sbjct: 186 AMYDFQAAE 194



>NP_115599
          Length = 2034

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AMYDIIAA 8
            A+YD IAA
Sbjct: 1665 ALYDAIAA 1672



>XP_006718613
          Length = 1946

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AMYDIIAA 8
            A+YD IAA
Sbjct: 1577 ALYDAIAA 1584



>XP_006718612
          Length = 2041

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AMYDIIAA 8
            A+YD IAA
Sbjct: 1672 ALYDAIAA 1679



>XP_005274044
          Length = 2046

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AMYDIIAA 8
            A+YD IAA
Sbjct: 1677 ALYDAIAA 1684



>XP_005274045
          Length = 2047

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    AMYDIIAA 8
            A+YD IAA
Sbjct: 1678 ALYDAIAA 1685



>NP_001127907
          Length = 443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   YDIIAAE 9
           YDIIA E
Sbjct: 210 YDIIARE 216



>NP_115639
          Length = 443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   YDIIAAE 9
           YDIIA E
Sbjct: 210 YDIIARE 216



>XP_006713848
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   YDIIAAE 9
           YDIIA E
Sbjct: 112 YDIIARE 118



>XP_006713847
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   YDIIAAE 9
           YDIIA E
Sbjct: 136 YDIIARE 142



>NP_055826
          Length = 1858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   MYDIIAA 8
           MYD++ A
Sbjct: 912 MYDVVGA 918



>NP_001185879
          Length = 1880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   MYDIIAA 8
           MYD++ A
Sbjct: 934 MYDVVGA 940



>NP_003464
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AMYDIIAAE 9
           A+YD  AAE
Sbjct: 217 AIYDFEAAE 225



>NP_277050
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   AMYDIIAAE 9
           A+YD++  E
Sbjct: 297 ALYDVLGIE 305



>XP_005252660
          Length = 490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AMYDIIAAE 9
           A+YD  AAE
Sbjct: 167 AIYDFEAAE 175



>XP_005267270
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   AMYDIIAAE 9
           A+YD++  E
Sbjct: 297 ALYDVLGIE 305



>XP_005267269
          Length = 618

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   AMYDIIAAE 9
           A+YD++  E
Sbjct: 252 ALYDVLGIE 260



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.133    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 353,887
Number of extensions: 287
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)