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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              18    1135
XP_006715292                                                           18    1135
XP_006716135                                                           17    4312
NP_689669                                                              16    5631
NP_001166242                                                           16    5631
NP_001166243                                                           16    5631
NP_001244336                                                           16    7355
NP_000409                                                              16    7355
NP_001244335                                                           16    7355
XP_005255366                                                           16    7355
XP_005255362                                                           16    7355
NP_001157745                                                           15    9605
NP_001074010                                                           15    9605
XP_005254311                                                           15    9605
XP_006720508                                                           15    9605
XP_006720507                                                           15    9605
XP_005254310                                                           15    9605
XP_005254309                                                           15    9605
XP_005254306                                                           15    9605
XP_005254305                                                           15    9605
XP_005254304                                                           15    9605
XP_005254303                                                           15    9605
XP_006720506                                                           15    9605
XP_005254302                                                           15    9605
XP_005254301                                                           15    9605
XP_006720505                                                           15    9605
XP_005254300                                                           15    9605

>NP_115927
          Length = 1321

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMFRVVNVT 9
           AMF VVNVT
Sbjct: 336 AMFGVVNVT 344



>XP_006715292
          Length = 759

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AMFRVVNVT 9
           AMF VVNVT
Sbjct: 323 AMFGVVNVT 331



>XP_006716135
          Length = 718

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  AMFRVVNVT 9
          A+F+VVNV+
Sbjct: 4  AIFQVVNVS 12



>NP_689669
          Length = 242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MFRVVNVT 9
          +FR+ NVT
Sbjct: 71 VFRIANVT 78



>NP_001166242
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MFRVVNVT 9
          +FR+ NVT
Sbjct: 71 VFRIANVT 78



>NP_001166243
          Length = 242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MFRVVNVT 9
          +FR+ NVT
Sbjct: 71 VFRIANVT 78



>NP_001244336
          Length = 810

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AMFRVVNVT 9
           A FR+ NVT
Sbjct: 155 ADFRIYNVT 163



>NP_000409
          Length = 825

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AMFRVVNVT 9
           A FR+ NVT
Sbjct: 170 ADFRIYNVT 178



>NP_001244335
          Length = 825

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AMFRVVNVT 9
           A FR+ NVT
Sbjct: 170 ADFRIYNVT 178



>XP_005255366
          Length = 364

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AMFRVVNVT 9
           A FR+ NVT
Sbjct: 230 ADFRIYNVT 238



>XP_005255362
          Length = 885

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AMFRVVNVT 9
           A FR+ NVT
Sbjct: 230 ADFRIYNVT 238



>NP_001157745
          Length = 3065

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2648 MPRIVNVT 2655



>NP_001074010
          Length = 2856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2439 MPRIVNVT 2446



>XP_005254311
          Length = 1627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 1210 MPRIVNVT 1217



>XP_006720508
          Length = 2658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2241 MPRIVNVT 2248



>XP_006720507
          Length = 2763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2346 MPRIVNVT 2353



>XP_005254310
          Length = 2906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2489 MPRIVNVT 2496



>XP_005254309
          Length = 2956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2539 MPRIVNVT 2546



>XP_005254306
          Length = 3066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2649 MPRIVNVT 2656



>XP_005254305
          Length = 3114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2697 MPRIVNVT 2704



>XP_005254304
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705



>XP_005254303
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705



>XP_006720506
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705



>XP_005254302
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705



>XP_005254301
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705



>XP_006720505
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705



>XP_005254300
          Length = 3115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MFRVVNVT 9
            M R+VNVT
Sbjct: 2698 MPRIVNVT 2705



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.138    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 277,869
Number of extensions: 374
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)