Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     299
NP_001035957                                                           20     299
XP_006721991                                                           20     299
XP_006721990                                                           20     299
XP_006721989                                                           20     299
XP_005258041                                                           20     299
XP_006721988                                                           20     299
XP_006721987                                                           20     299
XP_006721986                                                           20     299
XP_005258040                                                           20     299
XP_006721985                                                           20     299
NP_689503                                                              18    1935
NP_001005245                                                           17    3301
NP_001005284                                                           17    3301
NP_006734                                                              17    4312
NP_660333                                                              17    4312
NP_001171455                                                           17    4312
NP_055006                                                              17    4312
NP_006589                                                              17    4312
NP_079272                                                              17    4312
XP_006725069                                                           17    4312
XP_006719619                                                           17    4312
XP_005248288                                                           17    4312
XP_006713858                                                           17    4312
NP_001004740                                                           17    4312
NP_001004741                                                           17    4312
NP_079375                                                              16    5631
NP_001071096                                                           16    5631
NP_057106                                                              16    5631
NP_001230700                                                           16    5631
NP_036505                                                              16    5631
NP_001230701                                                           16    5631
XP_006721641                                                           16    5631
XP_006721640                                                           16    5631
XP_006711425                                                           16    5631
NP_055433                                                              16    5631
NP_001398                                                              16    7355
NP_004708                                                              16    7355
NP_001513                                                              16    7355
XP_006716236                                                           16    7355
XP_006716235                                                           16    7355
NP_078825                                                              15    9605
NP_001167446                                                           15    9605
NP_001076006                                                           15    9605
NP_001076007                                                           15    9605
NP_079235                                                              15    9605
NP_001167447                                                           15    9605
NP_149039                                                              15    9605
NP_001137542                                                           15    9605
NP_003777                                                              15    9605
NP_060440                                                              15    9605
XP_006719671                                                           15    9605
XP_006719670                                                           15    9605
XP_005253992                                                           15    9605
XP_005253991                                                           15    9605
XP_006719657                                                           15    9605
>NP_000258
          Length = 2818

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1387 AMFLRFINP 1395
>NP_001035957
          Length = 2839

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1408 AMFLRFINP 1416
>XP_006721991
          Length = 1632

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1418 AMFLRFINP 1426
>XP_006721990
          Length = 2823

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1418 AMFLRFINP 1426
>XP_006721989
          Length = 2823

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1418 AMFLRFINP 1426
>XP_005258041
          Length = 2836

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1387 AMFLRFINP 1395
>XP_006721988
          Length = 2846

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1397 AMFLRFINP 1405
>XP_006721987
          Length = 2849

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1418 AMFLRFINP 1426
>XP_006721986
          Length = 2854

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1405 AMFLRFINP 1413
>XP_005258040
          Length = 2857

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1408 AMFLRFINP 1416
>XP_006721985
          Length = 2867

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AMFLTFINP 9
            AMFL FINP
Sbjct: 1418 AMFLRFINP 1426
>NP_689503
          Length = 194

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 MFLTFINP 9
         MFLT +NP
Sbjct: 1 MFLTAVNP 8
>NP_001005245
          Length = 305

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AMFLTFINP 9
           A+F TF++P
Sbjct: 274 AVFYTFVSP 282
>NP_001005284
          Length = 327

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AMFLTFINP 9
           A+F T INP
Sbjct: 290 ALFYTVINP 298
>NP_006734
          Length = 157

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  FLTFINP 9
          F+TF NP
Sbjct: 51 FITFTNP 57
>NP_660333
          Length = 1321

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   AMFLTFIN 8
           A F+TF+N
Sbjct: 924 ASFITFVN 931
>NP_001171455
          Length = 1939

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MFLTFINP 9
           +F+TF NP
Sbjct: 147 VFMTFSNP 154
>NP_055006
          Length = 1980

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MFLTFINP 9
           +F+TF NP
Sbjct: 147 VFMTFSNP 154
>NP_006589
          Length = 1083

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MFLTFINP 9
           +FLT+I+P
Sbjct: 223 IFLTYISP 230
>NP_079272
          Length = 751

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FLTFIN 8
           FLTFIN
Sbjct: 169 FLTFIN 174
>XP_006725069
          Length = 1088

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MFLTFINP 9
           +FLT+I+P
Sbjct: 223 IFLTYISP 230
>XP_006719619
          Length = 1980

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MFLTFINP 9
           +F+TF NP
Sbjct: 147 VFMTFSNP 154
>XP_005248288
          Length = 1088

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MFLTFINP 9
           +FLT+I+P
Sbjct: 223 IFLTYISP 230
>XP_006713858
          Length = 712

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FLTFIN 8
           FLTFIN
Sbjct: 169 FLTFIN 174
>NP_001004740
          Length = 315

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AMFLTFINP 9
           A+F TF++P
Sbjct: 275 AVFYTFLSP 283
>NP_001004741
          Length = 315

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   AMFLTFINP 9
           A+F TF++P
Sbjct: 275 AVFYTFLSP 283
>NP_079375
          Length = 1217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLTFINP 9
            FLT +NP
Sbjct: 1186 FLTHVNP 1192
>NP_001071096
          Length = 265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           LTF+NP
Sbjct: 203 LTFLNP 208
>NP_057106
          Length = 247

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           LTF+NP
Sbjct: 203 LTFLNP 208
>NP_001230700
          Length = 190

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           LTF+NP
Sbjct: 146 LTFLNP 151
>NP_036505
          Length = 321

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MFLTFINP 9
           +F+T INP
Sbjct: 285 VFMTVINP 292
>NP_001230701
          Length = 195

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           LTF+NP
Sbjct: 133 LTFLNP 138
>XP_006721641
          Length = 1140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLTFINP 9
            FLT +NP
Sbjct: 1109 FLTHVNP 1115
>XP_006721640
          Length = 1174

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FLTFINP 9
            FLT +NP
Sbjct: 1143 FLTHVNP 1149
>XP_006711425
          Length = 208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           LTF+NP
Sbjct: 146 LTFLNP 151
>NP_055433
          Length = 761

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MFLTFINP 9
           MF  ++NP
Sbjct: 559 MFFVYVNP 566
>NP_001398
          Length = 3312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MFLTFINP 9
            M LT++NP
Sbjct: 2309 MELTYLNP 2316
>NP_004708
          Length = 1065

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   MFLTFINP 9
           MF+ ++NP
Sbjct: 396 MFMWYLNP 403
>NP_001513
          Length = 1108

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           +TFINP
Sbjct: 513 VTFINP 518
>XP_006716236
          Length = 1022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   MFLTFINP 9
           MF+ ++NP
Sbjct: 344 MFMWYLNP 351
>XP_006716235
          Length = 1057

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   MFLTFINP 9
           MF+ ++NP
Sbjct: 396 MFMWYLNP 403
>NP_078825
          Length = 573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 162 LSFINP 167
>NP_001167446
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 106 LSFINP 111
>NP_001076006
          Length = 587

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 162 LSFINP 167
>NP_001076007
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 162 LSFINP 167
>NP_079235
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AMFLTFI 7
           A+FLTF+
Sbjct: 212 AVFLTFL 218
>NP_001167447
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 102 LSFINP 107
>NP_149039
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MFLTFIN 8
           +FLTFI+
Sbjct: 111 LFLTFIS 117
>NP_001137542
          Length = 572

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 324 LSFINP 329
>NP_003777
          Length = 1527

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 324 LSFINP 329
>NP_060440
          Length = 1164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   LTFINP 9
           LTF NP
Sbjct: 677 LTFFNP 682
>XP_006719671
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AMFLTFI 7
           A+FLTF+
Sbjct: 212 AVFLTFL 218
>XP_006719670
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AMFLTFI 7
          A+FLTF+
Sbjct: 17 AVFLTFL 23
>XP_005253992
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 162 LSFINP 167
>XP_005253991
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 162 LSFINP 167
>XP_006719657
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LTFINP 9
           L+FINP
Sbjct: 106 LSFINP 111
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.150    0.478 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 373,803
Number of extensions: 835
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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