Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_114142                                                              17    4312
XP_006711003                                                           17    4312
NP_001035829                                                           16    7355
NP_001166996                                                           16    7355
NP_004591                                                              16    7355
NP_001166995                                                           16    7355
NP_001035828                                                           16    7355
XP_006711560                                                           16    7355
XP_006711559                                                           16    7355
XP_006711558                                                           16    7355
>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MAMSSIPM 9
            MAMSSIPM
Sbjct: 1935 MAMSSIPM 1942
>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MAMSSIPM 9
            MAMSSIPM
Sbjct: 1730 MAMSSIPM 1737
>NP_114142
          Length = 451

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AMAMSSIPM 9
           A+AM+S+P+
Sbjct: 170 ALAMASVPV 178
>XP_006711003
          Length = 451

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AMAMSSIPM 9
           A+AM+S+P+
Sbjct: 170 ALAMASVPV 178
>NP_001035829
          Length = 534

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   MAMSSIP 8
           MAMS IP
Sbjct: 435 MAMSQIP 441
>NP_001166996
          Length = 518

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   MAMSSIP 8
           MAMS IP
Sbjct: 435 MAMSQIP 441
>NP_004591
          Length = 538

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   MAMSSIP 8
           MAMS IP
Sbjct: 435 MAMSQIP 441
>NP_001166995
          Length = 538

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   MAMSSIP 8
           MAMS IP
Sbjct: 435 MAMSQIP 441
>NP_001035828
          Length = 525

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   MAMSSIP 8
           MAMS IP
Sbjct: 435 MAMSQIP 441
>XP_006711560
          Length = 538

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   MAMSSIP 8
           MAMS IP
Sbjct: 435 MAMSQIP 441
>XP_006711559
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   MAMSSIP 8
           MAMS IP
Sbjct: 457 MAMSQIP 463
>XP_006711558
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   MAMSSIP 8
           MAMS IP
Sbjct: 457 MAMSQIP 463
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.113    0.290 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 334,647
Number of extensions: 150
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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