Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              18    1135
NP_001035957                                                           18    1135
XP_006721990                                                           18    1135
XP_006721989                                                           18    1135
XP_005258041                                                           18    1135
XP_006721988                                                           18    1135
XP_006721987                                                           18    1135
XP_006721986                                                           18    1135
XP_005258040                                                           18    1135
XP_006721985                                                           18    1135
NP_001073883                                                           17    2528
XP_005267748                                                           17    2528
NP_689916                                                              17    3301
XP_006715953                                                           17    3301
XP_006715952                                                           17    3301
XP_005250250                                                           17    3301
XP_006715951                                                           17    3301
XP_005250248                                                           17    3301
NP_060594                                                              16    5631
XP_006721290                                                           16    5631
XP_005256079                                                           16    5631
XP_005256078                                                           16    5631
NP_001274152                                                           16    7355
NP_002408                                                              16    7355
NP_001139438                                                           16    7355
NP_054858                                                              16    7355
XP_006717927                                                           16    7355
NP_006531                                                              15    9605
NP_001172003                                                           15    9605
NP_001171988                                                           15    9605
NP_078893                                                              15    9605
NP_065910                                                              15    9605
NP_775901                                                              15    9605
XP_005266736                                                           15    9605
XP_006722498                                                           15    9605
XP_006722497                                                           15    9605
XP_006717349                                                           15    9605
XP_005252266                                                           15    9605
XP_005252265                                                           15    9605
XP_005252264                                                           15    9605
XP_006717348                                                           15    9605
XP_006717347                                                           15    9605
XP_006717346                                                           15    9605
XP_005251190                                                           15    9605
XP_005251188                                                           15    9605
XP_005251189                                                           15    9605
XP_005251187                                                           15    9605
XP_005251186                                                           15    9605
XP_005251185                                                           15    9605
XP_005263222                                                           15    9605
XP_005263221                                                           15    9605
XP_005263220                                                           15    9605
XP_006714345                                                           15    9605
XP_005263219                                                           15    9605
>NP_000258
          Length = 2818

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2177 ALTSLETVT 2185
>NP_001035957
          Length = 2839

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2198 ALTSLETVT 2206
>XP_006721990
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2208 ALTSLETVT 2216
>XP_006721989
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2208 ALTSLETVT 2216
>XP_005258041
          Length = 2836

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2177 ALTSLETVT 2185
>XP_006721988
          Length = 2846

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2187 ALTSLETVT 2195
>XP_006721987
          Length = 2849

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2208 ALTSLETVT 2216
>XP_006721986
          Length = 2854

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2195 ALTSLETVT 2203
>XP_005258040
          Length = 2857

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2198 ALTSLETVT 2206
>XP_006721985
          Length = 2867

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALTSLETAT 9
            ALTSLET T
Sbjct: 2208 ALTSLETVT 2216
>NP_001073883
          Length = 2028

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LTSLETAT 9
           +TSLETAT
Sbjct: 651 VTSLETAT 658
>XP_005267748
          Length = 1535

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LTSLETAT 9
           +TSLETAT
Sbjct: 651 VTSLETAT 658
>NP_689916
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALTSLETAT 9
           ALT+ ETAT
Sbjct: 201 ALTNTETAT 209
>XP_006715953
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALTSLETAT 9
           ALT+ ETAT
Sbjct: 201 ALTNTETAT 209
>XP_006715952
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALTSLETAT 9
           ALT+ ETAT
Sbjct: 201 ALTNTETAT 209
>XP_005250250
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALTSLETAT 9
           ALT+ ETAT
Sbjct: 201 ALTNTETAT 209
>XP_006715951
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALTSLETAT 9
           ALT+ ETAT
Sbjct: 201 ALTNTETAT 209
>XP_005250248
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ALTSLETAT 9
           ALT+ ETAT
Sbjct: 201 ALTNTETAT 209
>NP_060594
          Length = 774

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET T
Sbjct: 524 LTSLETNT 531
>XP_006721290
          Length = 774

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET T
Sbjct: 524 LTSLETNT 531
>XP_005256079
          Length = 774

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET T
Sbjct: 524 LTSLETNT 531
>XP_005256078
          Length = 774

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET T
Sbjct: 524 LTSLETNT 531
>NP_001274152
          Length = 1791

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ALTSLETA 8
           +LTSLET+
Sbjct: 444 SLTSLETS 451
>NP_002408
          Length = 3256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           +TSLET T
Sbjct: 851 MTSLETKT 858
>NP_001139438
          Length = 2896

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           +TSLET T
Sbjct: 491 MTSLETKT 498
>NP_054858
          Length = 1791

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ALTSLETA 8
           +LTSLET+
Sbjct: 444 SLTSLETS 451
>XP_006717927
          Length = 2406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 LTSLETAT 9
         +TSLET T
Sbjct: 1 MTSLETKT 8
>NP_006531
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTSLETAT 9
           +TSL+T+T
Sbjct: 280 ITSLDTST 287
>NP_001172003
          Length = 1259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>NP_001171988
          Length = 1495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>NP_078893
          Length = 1495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>NP_065910
          Length = 1996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSLETAT 9
           +SLETAT
Sbjct: 367 SSLETAT 373
>NP_775901
          Length = 2226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    ALTSLET 7
            ALT+LET
Sbjct: 1392 ALTTLET 1398
>XP_005266736
          Length = 1314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   ALTSLET 7
           ALT+LET
Sbjct: 480 ALTTLET 486
>XP_006722498
          Length = 2059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    ALTSLET 7
            ALT+LET
Sbjct: 1393 ALTTLET 1399
>XP_006722497
          Length = 2227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    ALTSLET 7
            ALT+LET
Sbjct: 1393 ALTTLET 1399
>XP_006717349
          Length = 1046

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>XP_005252266
          Length = 1046

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>XP_005252265
          Length = 1381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>XP_005252264
          Length = 1478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>XP_006717348
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>XP_006717347
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>XP_006717346
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LTSLETAT 9
           LTSLET +
Sbjct: 159 LTSLETTS 166
>XP_005251190
          Length = 1395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTSLETAT 9
           +TSL+T+T
Sbjct: 280 ITSLDTST 287
>XP_005251188
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTSLETAT 9
           +TSL+T+T
Sbjct: 280 ITSLDTST 287
>XP_005251189
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTSLETAT 9
           +TSL+T+T
Sbjct: 280 ITSLDTST 287
>XP_005251187
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTSLETAT 9
           +TSL+T+T
Sbjct: 280 ITSLDTST 287
>XP_005251186
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTSLETAT 9
           +TSL+T+T
Sbjct: 280 ITSLDTST 287
>XP_005251185
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTSLETAT 9
           +TSL+T+T
Sbjct: 280 ITSLDTST 287
>XP_005263222
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSLETAT 9
           +SLETAT
Sbjct: 191 SSLETAT 197
>XP_005263221
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSLETAT 9
           +SLETAT
Sbjct: 191 SSLETAT 197
>XP_005263220
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSLETAT 9
           +SLETAT
Sbjct: 191 SSLETAT 197
>XP_006714345
          Length = 1961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSLETAT 9
           +SLETAT
Sbjct: 332 SSLETAT 338
>XP_005263219
          Length = 1996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSLETAT 9
           +SLETAT
Sbjct: 367 SSLETAT 373
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.102    0.225 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 261,073
Number of extensions: 381
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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