Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1482
XP_006716142                                                           18    1482
XP_005250088                                                           18    1482
XP_006716141                                                           18    1482
XP_005250085                                                           18    1482
XP_006716140                                                           18    1482
XP_005250084                                                           18    1482
XP_005250083                                                           18    1482
XP_005250082                                                           18    1482
NP_008913                                                              16    5631
NP_065770                                                              16    5631
NP_872393                                                              16    5631
XP_005276260                                                           16    5631
XP_005269222                                                           16    5631
XP_006712515                                                           16    5631
XP_006712513                                                           16    5631
XP_006712512                                                           16    5631
XP_006712511                                                           16    5631
XP_006712510                                                           16    5631
XP_006712509                                                           16    5631
XP_005246533                                                           16    5631
XP_006712508                                                           16    5631
XP_005264734                                                           16    5631
XP_005275976                                                           16    5631
NP_115893                                                              16    5631
NP_671725                                                              16    7355
NP_757379                                                              16    7355
NP_597719                                                              16    7355
NP_001171922                                                           16    7355
NP_001274730                                                           16    7355
XP_006719916                                                           16    7355
XP_006719915                                                           16    7355
XP_006719914                                                           16    7355
XP_005266530                                                           16    7355
XP_006719913                                                           16    7355
XP_006719912                                                           16    7355
XP_006719911                                                           16    7355
XP_006719910                                                           16    7355
XP_006719909                                                           16    7355
XP_006713569                                                           16    7355
XP_006710418                                                           16    7355
NP_112567                                                              16    7355
NP_001071159                                                           15    9605
NP_079016                                                              15    9605
NP_612504                                                              15    9605
NP_065776                                                              15    9605
XP_006718976                                                           15    9605
XP_005277780                                                           15    9605
XP_006715966                                                           15    9605
XP_005250277                                                           15    9605
XP_006715965                                                           15    9605
XP_005250276                                                           15    9605
XP_006715964                                                           15    9605
XP_006715963                                                           15    9605
>NP_733751
          Length = 4911

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALSAKQHTA 9
            ALSAKQ TA
Sbjct: 3211 ALSAKQRTA 3219
>XP_006716142
          Length = 4927

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALSAKQHTA 9
            ALSAKQ TA
Sbjct: 3228 ALSAKQRTA 3236
>XP_005250088
          Length = 4928

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALSAKQHTA 9
            ALSAKQ TA
Sbjct: 3228 ALSAKQRTA 3236
>XP_006716141
          Length = 4959

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALSAKQHTA 9
            ALSAKQ TA
Sbjct: 3228 ALSAKQRTA 3236
>XP_005250085
          Length = 4979

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALSAKQHTA 9
            ALSAKQ TA
Sbjct: 3228 ALSAKQRTA 3236
>XP_006716140
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALSAKQHTA 9
            ALSAKQ TA
Sbjct: 3228 ALSAKQRTA 3236
>XP_005250084
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALSAKQHTA 9
            ALSAKQ TA
Sbjct: 3228 ALSAKQRTA 3236
>XP_005250083
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALSAKQHTA 9
            ALSAKQ TA
Sbjct: 3227 ALSAKQRTA 3235
>XP_005250082
          Length = 4983

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALSAKQHTA 9
            ALSAKQ TA
Sbjct: 3228 ALSAKQRTA 3236
>NP_008913
          Length = 326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LSAKQHTA 9
           + AK+HTA
Sbjct: 313 MKAKEHTA 320
>NP_065770
          Length = 2036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ALSAKQHTA 9
           ALSA QH A
Sbjct: 147 ALSAYQHPA 155
>NP_872393
          Length = 3234

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2865 LSARQHIA 2872
>XP_005276260
          Length = 160

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  ALSAKQH 7
          ALSA+QH
Sbjct: 34 ALSAQQH 40
>XP_005269222
          Length = 326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LSAKQHTA 9
           + AK+HTA
Sbjct: 313 MKAKEHTA 320
>XP_006712515
          Length = 3232

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2863 LSARQHIA 2870
>XP_006712513
          Length = 3220

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2832 LSARQHIA 2839
>XP_006712512
          Length = 3237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2868 LSARQHIA 2875
>XP_006712511
          Length = 3256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2868 LSARQHIA 2875
>XP_006712510
          Length = 3305

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2936 LSARQHIA 2943
>XP_006712509
          Length = 3319

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2931 LSARQHIA 2938
>XP_005246533
          Length = 3323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2935 LSARQHIA 2942
>XP_006712508
          Length = 3324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2936 LSARQHIA 2943
>XP_005264734
          Length = 160

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  ALSAKQH 7
          ALSA+QH
Sbjct: 34 ALSAQQH 40
>XP_005275976
          Length = 160

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  ALSAKQH 7
          ALSA+QH
Sbjct: 34 ALSAQQH 40
>NP_115893
          Length = 3258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSAKQHTA 9
            LSA+QH A
Sbjct: 2870 LSARQHIA 2877
>NP_671725
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LSAKQHTA 9
           L AKQH A
Sbjct: 366 LRAKQHAA 373
>NP_757379
          Length = 377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LSAKQHTA 9
           L AKQH A
Sbjct: 361 LRAKQHAA 368
>NP_597719
          Length = 770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ALSAKQHT 8
           ALS+K+H+
Sbjct: 731 ALSSKEHS 738
>NP_001171922
          Length = 1619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 541 SAKQHIA 547
>NP_001274730
          Length = 578

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LSAKQHTA 9
           +SAK HT+
Sbjct: 257 ISAKDHTS 264
>XP_006719916
          Length = 1482

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 400 SAKQHIA 406
>XP_006719915
          Length = 1531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 400 SAKQHIA 406
>XP_006719914
          Length = 1639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 561 SAKQHIA 567
>XP_005266530
          Length = 1643

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 561 SAKQHIA 567
>XP_006719913
          Length = 1650

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 561 SAKQHIA 567
>XP_006719912
          Length = 1672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 541 SAKQHIA 547
>XP_006719911
          Length = 1688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 561 SAKQHIA 567
>XP_006719910
          Length = 1692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 561 SAKQHIA 567
>XP_006719909
          Length = 1692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 561 SAKQHIA 567
>XP_006713569
          Length = 341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ALSAKQHT 8
           ALS+K+H+
Sbjct: 302 ALSSKEHS 309
>XP_006710418
          Length = 377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LSAKQHTA 9
           L AKQH A
Sbjct: 361 LRAKQHAA 368
>NP_112567
          Length = 1623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SAKQHTA 9
           SAKQH A
Sbjct: 541 SAKQHIA 547
>NP_001071159
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ALSAKQHT 8
           ALSA QH+
Sbjct: 215 ALSALQHS 222
>NP_079016
          Length = 618

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ALSAKQHT 8
           ALSA QH+
Sbjct: 386 ALSALQHS 393
>NP_612504
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  SAKQHT 8
          S+KQHT
Sbjct: 52 SSKQHT 57
>NP_065776
          Length = 861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAKQHT 8
           S+KQHT
Sbjct: 176 SSKQHT 181
>XP_006718976
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ALSAKQHT 8
           ALSA QH+
Sbjct: 386 ALSALQHS 393
>XP_005277780
          Length = 629

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ALSAKQHT 8
           ALSA QH+
Sbjct: 386 ALSALQHS 393
>XP_006715966
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAKQHT 8
           S+KQHT
Sbjct: 176 SSKQHT 181
>XP_005250277
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAKQHT 8
           S+KQHT
Sbjct: 176 SSKQHT 181
>XP_006715965
          Length = 872

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAKQHT 8
           S+KQHT
Sbjct: 176 SSKQHT 181
>XP_005250276
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAKQHT 8
           S+KQHT
Sbjct: 176 SSKQHT 181
>XP_006715964
          Length = 964

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAKQHT 8
           S+KQHT
Sbjct: 176 SSKQHT 181
>XP_006715963
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SAKQHT 8
           S+KQHT
Sbjct: 176 SSKQHT 181
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.102    0.253 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 521,753
Number of extensions: 380
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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