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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              18    1935
NP_001004471                                                           17    4312
NP_001252542                                                           16    7355
NP_001010985                                                           16    7355
XP_006710676                                                           16    7355
NP_065099                                                              15    9605
NP_001275913                                                           15    9605
NP_060585                                                              15    9605
NP_001275912                                                           15    9605
XP_005263162                                                           15    9605
XP_005263159                                                           15    9605
NP_064529                                                              15    9605

>NP_004251
          Length = 1208

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALRSTLLRK 9
           ALRS LLRK
Sbjct: 368 ALRSRLLRK 376



>NP_001004471
          Length = 319

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALRSTLLRK 9
           ALRS ++RK
Sbjct: 305 ALRSAIIRK 313



>NP_001252542
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ALRSTLLRK 9
          ALR T +RK
Sbjct: 58 ALRQTYIRK 66



>NP_001010985
          Length = 354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ALRSTLLRK 9
          ALR T +RK
Sbjct: 58 ALRQTYIRK 66



>XP_006710676
          Length = 354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ALRSTLLRK 9
          ALR T +RK
Sbjct: 58 ALRQTYIRK 66



>NP_065099
          Length = 1286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LRSTLLR 8
          LR+TLLR
Sbjct: 74 LRTTLLR 80



>NP_001275913
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALRSTLLRK 9
           ALR  LL+K
Sbjct: 577 ALRDALLKK 585



>NP_060585
          Length = 687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALRSTLLRK 9
           ALR  LL+K
Sbjct: 674 ALRDALLKK 682



>NP_001275912
          Length = 650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALRSTLLRK 9
           ALR  LL+K
Sbjct: 637 ALRDALLKK 645



>XP_005263162
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALRSTLLRK 9
           ALR  LL+K
Sbjct: 577 ALRDALLKK 585



>XP_005263159
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALRSTLLRK 9
           ALR  LL+K
Sbjct: 649 ALRDALLKK 657



>NP_064529
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALRSTLLRK 9
           A+ STL+RK
Sbjct: 264 AVASTLVRK 272



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.134    0.321 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 326,544
Number of extensions: 405
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)