Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              17    4312
NP_061184                                                              17    4312
NP_001093146                                                           16    7355
NP_110417                                                              16    7355
NP_001164104                                                           16    7355
NP_005600                                                              15    9605
NP_056138                                                              15    9605
NP_006280                                                              15    9605
NP_001158188                                                           15    9605
NP_001077081                                                           15    9605
NP_001013641                                                           15    9605
NP_065870                                                              15    9605
NP_001243352                                                           15    9605
NP_116289                                                              15    9605
NP_659465                                                              15    9605
NP_001191229                                                           15    9605
NP_001273503                                                           15    9605
NP_001273505                                                           15    9605
NP_001257638                                                           15    9605
XP_006716915                                                           15    9605
XP_005251621                                                           15    9605
XP_005248808                                                           15    9605
XP_005248807                                                           15    9605
XP_005248806                                                           15    9605
XP_005248805                                                           15    9605
XP_005248804                                                           15    9605
XP_005248803                                                           15    9605
XP_005248801                                                           15    9605
XP_005248800                                                           15    9605
XP_006715081                                                           15    9605
XP_005248977                                                           15    9605
XP_005248976                                                           15    9605
>NP_005350
          Length = 552

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALQLLYEVL 9
           ALQLL EVL
Sbjct: 532 ALQLLDEVL 540
>NP_061184
          Length = 980

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ALQLLYEV 8
           ALQLLY++
Sbjct: 806 ALQLLYDL 813
>NP_001093146
          Length = 462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALQLLYEVL 9
           A  LLYE+L
Sbjct: 330 ATALLYEIL 338
>NP_110417
          Length = 612

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ALQLLYEVL 9
           ALQL Y VL
Sbjct: 495 ALQLPYNVL 503
>NP_001164104
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALQLLYEVL 9
           A  LLYE+L
Sbjct: 490 ATALLYEIL 498
>NP_005600
          Length = 842

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   LQLLYEV 8
           LQ++YE+
Sbjct: 401 LQIIYEI 407
>NP_056138
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QLLYEVL 9
           QLLY VL
Sbjct: 215 QLLYSVL 221
>NP_006280
          Length = 2541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ALQLLY 6
            ALQLLY
Sbjct: 1772 ALQLLY 1777
>NP_001158188
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   LQLLYEV 8
           LQ++YE+
Sbjct: 313 LQIIYEI 319
>NP_001077081
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QLLYEV 8
           QLLYE+
Sbjct: 142 QLLYEI 147
>NP_001013641
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QLLYEV 8
           QLLYE+
Sbjct: 142 QLLYEI 147
>NP_065870
          Length = 1302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LQLLYEVL 9
           +QLLYE L
Sbjct: 236 MQLLYERL 243
>NP_001243352
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALQLLYEVL 9
           ALQLL+ +L
Sbjct: 218 ALQLLFLIL 226
>NP_116289
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALQLLYEVL 9
           ALQLL+ +L
Sbjct: 227 ALQLLFLIL 235
>NP_659465
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LLYEVL 9
           LLYEVL
Sbjct: 274 LLYEVL 279
>NP_001191229
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALQLLYEVL 9
           ALQLL+ +L
Sbjct: 227 ALQLLFLIL 235
>NP_001273503
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LLYEVL 9
           LLYEVL
Sbjct: 247 LLYEVL 252
>NP_001273505
          Length = 330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LLYEVL 9
           LLYEVL
Sbjct: 237 LLYEVL 242
>NP_001257638
          Length = 203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALQLLYEVL 9
           ALQ+ Y VL
Sbjct: 108 ALQVYYHVL 116
>XP_006716915
          Length = 2542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ALQLLY 6
            ALQLLY
Sbjct: 1772 ALQLLY 1777
>XP_005251621
          Length = 2542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ALQLLY 6
            ALQLLY
Sbjct: 1772 ALQLLY 1777
>XP_005248808
          Length = 1121

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LQLLYEVL 9
           +QLLYE L
Sbjct: 279 MQLLYERL 286
>XP_005248807
          Length = 1182

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LQLLYEVL 9
           +QLLYE L
Sbjct: 116 MQLLYERL 123
>XP_005248806
          Length = 1317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LQLLYEVL 9
           +QLLYE L
Sbjct: 279 MQLLYERL 286
>XP_005248805
          Length = 1345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LQLLYEVL 9
           +QLLYE L
Sbjct: 279 MQLLYERL 286
>XP_005248804
          Length = 1378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LQLLYEVL 9
           +QLLYE L
Sbjct: 116 MQLLYERL 123
>XP_005248803
          Length = 1455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LQLLYEVL 9
           +QLLYE L
Sbjct: 193 MQLLYERL 200
>XP_005248801
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LQLLYEVL 9
           +QLLYE L
Sbjct: 279 MQLLYERL 286
>XP_005248800
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LQLLYEVL 9
           +QLLYE L
Sbjct: 279 MQLLYERL 286
>XP_006715081
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LLYEVL 9
           LLYEVL
Sbjct: 160 LLYEVL 165
>XP_005248977
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LLYEVL 9
           LLYEVL
Sbjct: 274 LLYEVL 279
>XP_005248976
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LLYEVL 9
           LLYEVL
Sbjct: 274 LLYEVL 279
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.149    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 445,618
Number of extensions: 513
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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