Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1135
NP_001263689                                                           18    1135
NP_001119584                                                           18    1135
NP_001119586                                                           18    1135
NP_001263625                                                           18    1135
NP_001119590                                                           18    1135
NP_001263690                                                           18    1135
NP_001119585                                                           18    1135
NP_001263624                                                           18    1135
NP_006695                                                              17    3301
NP_001124384                                                           17    3301
NP_037395                                                              17    4312
NP_001167455                                                           17    4312
XP_005255969                                                           17    4312
XP_005255968                                                           17    4312
NP_065103                                                              16    5631
NP_037385                                                              16    5631
NP_001018067                                                           16    5631
NP_056965                                                              16    5631
NP_073617                                                              16    5631
XP_006718243                                                           16    5631
XP_006718242                                                           16    5631
XP_006718241                                                           16    5631
XP_006718240                                                           16    5631
NP_443732                                                              16    5631
NP_078966                                                              15    9605
NP_602296                                                              15    9605
NP_060886                                                              15    9605
XP_006723004                                                           15    9605
XP_005253488                                                           15    9605
XP_005253487                                                           15    9605
XP_005253782                                                           15    9605
XP_006719060                                                           15    9605
XP_006719059                                                           15    9605
XP_006719058                                                           15    9605
XP_005253778                                                           15    9605
XP_005253777                                                           15    9605
>NP_000537
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           ALNKMF QL
Sbjct: 129 ALNKMFCQL 137
>NP_001263689
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  ALNKMFRQL 9
          ALNKMF QL
Sbjct: 90 ALNKMFCQL 98
>NP_001119584
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           ALNKMF QL
Sbjct: 129 ALNKMFCQL 137
>NP_001119586
          Length = 341

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           ALNKMF QL
Sbjct: 129 ALNKMFCQL 137
>NP_001263625
          Length = 302

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  ALNKMFRQL 9
          ALNKMF QL
Sbjct: 90 ALNKMFCQL 98
>NP_001119590
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  ALNKMFRQL 9
          ALNKMF QL
Sbjct: 90 ALNKMFCQL 98
>NP_001263690
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  ALNKMFRQL 9
          ALNKMF QL
Sbjct: 90 ALNKMFCQL 98
>NP_001119585
          Length = 346

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           ALNKMF QL
Sbjct: 129 ALNKMFCQL 137
>NP_001263624
          Length = 307

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  ALNKMFRQL 9
          ALNKMF QL
Sbjct: 90 ALNKMFCQL 98
>NP_006695
          Length = 333

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ALNKMFRQL 9
           ALN++F+Q+
Sbjct: 276 ALNRLFQQI 284
>NP_001124384
          Length = 365

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ALNKMFRQL 9
           ALN++F+Q+
Sbjct: 308 ALNRLFQQI 316
>NP_037395
          Length = 651

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           ALN++ RQL
Sbjct: 139 ALNQLMRQL 147
>NP_001167455
          Length = 652

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           ALN++ RQL
Sbjct: 139 ALNQLMRQL 147
>XP_005255969
          Length = 615

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           ALN++ RQL
Sbjct: 139 ALNQLMRQL 147
>XP_005255968
          Length = 616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           ALN++ RQL
Sbjct: 139 ALNQLMRQL 147
>NP_065103
          Length = 396

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ALNKMFRQ 8
           A+N+ FRQ
Sbjct: 372 AMNRQFRQ 379
>NP_037385
          Length = 350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LNKMFRQL 9
          LN+MFR++
Sbjct: 47 LNEMFREV 54
>NP_001018067
          Length = 350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LNKMFRQL 9
          LN+MFR++
Sbjct: 47 LNEMFREV 54
>NP_056965
          Length = 350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LNKMFRQL 9
          LN+MFR++
Sbjct: 47 LNEMFREV 54
>NP_073617
          Length = 391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   ALNKMF 6
           ALNKMF
Sbjct: 362 ALNKMF 367
>XP_006718243
          Length = 216

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LNKMFRQL 9
           LN+MFR++
Sbjct: 110 LNEMFREV 117
>XP_006718242
          Length = 258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LNKMFRQL 9
           LN+MFR++
Sbjct: 110 LNEMFREV 117
>XP_006718241
          Length = 350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LNKMFRQL 9
          LN+MFR++
Sbjct: 47 LNEMFREV 54
>XP_006718240
          Length = 413

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LNKMFRQL 9
           LN+MFR++
Sbjct: 110 LNEMFREV 117
>NP_443732
          Length = 358

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KMFRQL 9
           KMFRQL
Sbjct: 115 KMFRQL 120
>NP_078966
          Length = 14507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1     ALNKMFR 7
             ALN++FR
Sbjct: 14356 ALNQLFR 14362
>NP_602296
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK+ RQL
Sbjct: 174 SLNKLPRQL 182
>NP_060886
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK FR++
Sbjct: 119 SLNKCFRKV 127
>XP_006723004
          Length = 6443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    ALNKMFR 7
            ALN++FR
Sbjct: 6292 ALNQLFR 6298
>XP_005253488
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK FR++
Sbjct: 119 SLNKCFRKV 127
>XP_005253487
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK FR++
Sbjct: 119 SLNKCFRKV 127
>XP_005253782
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK+ RQL
Sbjct: 174 SLNKLPRQL 182
>XP_006719060
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK+ RQL
Sbjct: 174 SLNKLPRQL 182
>XP_006719059
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK+ RQL
Sbjct: 174 SLNKLPRQL 182
>XP_006719058
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK+ RQL
Sbjct: 174 SLNKLPRQL 182
>XP_005253778
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK+ RQL
Sbjct: 174 SLNKLPRQL 182
>XP_005253777
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ALNKMFRQL 9
           +LNK+ RQL
Sbjct: 174 SLNKLPRQL 182
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.141    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 357,989
Number of extensions: 613
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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