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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_005018                                                              17    4312
NP_542437                                                              16    5631
NP_061197                                                              16    5631
NP_542435                                                              16    5631
NP_542436                                                              16    5631
NP_001010904                                                           16    5631
NP_079229                                                              16    5631
XP_005253995                                                           16    5631
XP_006719669                                                           16    5631
NP_060897                                                              16    7355
XP_006725262                                                           16    7355
XP_006725261                                                           16    7355
XP_006725260                                                           16    7355
XP_005255434                                                           16    7355
XP_005255433                                                           16    7355
XP_005255432                                                           16    7355
NP_001267489                                                           15    9605
NP_002080                                                              15    9605
NP_777592                                                              15    9605

>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ALMVEKMLK 9
            AL+VEKMLK
Sbjct: 3444 ALVVEKMLK 3452



>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ALMVEKMLK 9
            AL+VEKMLK
Sbjct: 3444 ALVVEKMLK 3452



>NP_005018
          Length = 728

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   ALMVEKMLK 9
           AL+VEK+L+
Sbjct: 281 ALLVEKLLQ 289



>NP_542437
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ALMVEKML 8
           A+M+EKM+
Sbjct: 566 AVMIEKMI 573



>NP_061197
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ALMVEKML 8
           A+M+EKM+
Sbjct: 566 AVMIEKMI 573



>NP_542435
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ALMVEKML 8
           A+M+EKM+
Sbjct: 566 AVMIEKMI 573



>NP_542436
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ALMVEKML 8
           A+M+EKM+
Sbjct: 566 AVMIEKMI 573



>NP_001010904
          Length = 288

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  LMVEKMLK 9
          L++EKMLK
Sbjct: 11 LILEKMLK 18



>NP_079229
          Length = 972

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MVEKMLK 9
           MVEKM+K
Sbjct: 180 MVEKMVK 186



>XP_005253995
          Length = 836

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  MVEKMLK 9
          MVEKM+K
Sbjct: 44 MVEKMVK 50



>XP_006719669
          Length = 944

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MVEKMLK 9
           MVEKM+K
Sbjct: 152 MVEKMVK 158



>NP_060897
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMVEKMLK 9
           L++EK+LK
Sbjct: 246 LIIEKLLK 253



>XP_006725262
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LMVEKMLK 9
          L++EK+LK
Sbjct: 34 LIIEKLLK 41



>XP_006725261
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LMVEKMLK 9
          L++EK+LK
Sbjct: 34 LIIEKLLK 41



>XP_006725260
          Length = 621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMVEKMLK 9
           L++EK+LK
Sbjct: 216 LIIEKLLK 223



>XP_005255434
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LMVEKMLK 9
          L++EK+LK
Sbjct: 34 LIIEKLLK 41



>XP_005255433
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LMVEKMLK 9
          L++EK+LK
Sbjct: 34 LIIEKLLK 41



>XP_005255432
          Length = 621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMVEKMLK 9
           L++EK+LK
Sbjct: 216 LIIEKLLK 223



>NP_001267489
          Length = 165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALMVEKMLK 9
           ALM  KM+K
Sbjct: 108 ALMAMKMMK 116



>NP_002080
          Length = 107

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MVEKMLK 9
           ++EKMLK
Sbjct: 96  IIEKMLK 102



>NP_777592
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  MVEKMLK 9
          M+E+MLK
Sbjct: 49 MIEQMLK 55



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.132    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 327,122
Number of extensions: 411
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)