Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055099                                                              17    4312
NP_001014839                                                           17    4312
NP_060334                                                              17    4312
XP_006716665                                                           17    4312
NP_001014841                                                           17    4312
NP_037418                                                              16    7355
XP_006717893                                                           16    7355
XP_006717891                                                           16    7355
XP_006717890                                                           16    7355
XP_005269849                                                           16    7355
XP_006717888                                                           16    7355
XP_006717887                                                           16    7355
XP_006717886                                                           16    7355
XP_006717885                                                           16    7355
XP_006717884                                                           16    7355
XP_005269836                                                           16    7355
>NP_055099
          Length = 729

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   ALKVAARL 8
           ALK+AARL
Sbjct: 281 ALKLAARL 288
>NP_001014839
          Length = 729

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   ALKVAARL 8
           ALK+AARL
Sbjct: 281 ALKLAARL 288
>NP_060334
          Length = 995

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  ALKVAARLR 9
          ALKVAA L+
Sbjct: 85 ALKVAAHLK 93
>XP_006716665
          Length = 943

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  ALKVAARLR 9
          ALKVAA L+
Sbjct: 85 ALKVAAHLK 93
>NP_001014841
          Length = 712

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   ALKVAARL 8
           ALK+AARL
Sbjct: 264 ALKLAARL 271
>NP_037418
          Length = 890

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LK+A RLR
Sbjct: 763 LKIAQRLR 770
>XP_006717893
          Length = 1233

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 698 LKVAARSR 705
>XP_006717891
          Length = 1143

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 607 LKVAARSR 614
>XP_006717890
          Length = 1145

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 609 LKVAARSR 616
>XP_005269849
          Length = 1207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 698 LKVAARSR 705
>XP_006717888
          Length = 1210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 702 LKVAARSR 709
>XP_006717887
          Length = 1211

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 702 LKVAARSR 709
>XP_006717886
          Length = 1234

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 698 LKVAARSR 705
>XP_006717885
          Length = 1237

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 702 LKVAARSR 709
>XP_006717884
          Length = 1238

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 702 LKVAARSR 709
>XP_005269836
          Length = 1238

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LKVAARLR 9
           LKVAAR R
Sbjct: 702 LKVAARSR 709
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.136    0.326 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 220,768
Number of extensions: 243
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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