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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              17    3301
NP_733762                                                              17    3301
XP_006713860                                                           17    3301
XP_006713859                                                           17    3301
XP_005247910                                                           17    3301
XP_005247909                                                           17    3301
NP_001008540                                                           16    7355
NP_003458                                                              16    7355
NP_000307                                                              15    9605
NP_001171673                                                           15    9605
NP_003499                                                              15    9605
XP_005265401                                                           15    9605
XP_005265400                                                           15    9605
XP_005265399                                                           15    9605
XP_006713335                                                           15    9605

>NP_005179
          Length = 906

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALKSTIVLT 9
           ALKSTI LT
Sbjct: 223 ALKSTIDLT 231



>NP_733762
          Length = 982

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALKSTIVLT 9
           ALKSTI LT
Sbjct: 215 ALKSTIDLT 223



>XP_006713860
          Length = 914

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALKSTIVLT 9
           ALKSTI LT
Sbjct: 243 ALKSTIDLT 251



>XP_006713859
          Length = 966

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALKSTIVLT 9
           ALKSTI LT
Sbjct: 243 ALKSTIDLT 251



>XP_005247910
          Length = 982

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALKSTIVLT 9
           ALKSTI LT
Sbjct: 215 ALKSTIDLT 223



>XP_005247909
          Length = 1010

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALKSTIVLT 9
           ALKSTI LT
Sbjct: 243 ALKSTIDLT 251



>NP_001008540
          Length = 356

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ALKSTIVL 8
           ALK+T++L
Sbjct: 241 ALKTTVIL 248



>NP_003458
          Length = 352

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ALKSTIVL 8
           ALK+T++L
Sbjct: 237 ALKTTVIL 244



>NP_000307
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LKSTIVL 8
           LKST+VL
Sbjct: 407 LKSTLVL 413



>NP_001171673
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LKSTIVL 8
           LKST+VL
Sbjct: 407 LKSTLVL 413



>NP_003499
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ALKSTIVLT 9
           ALK+ ++LT
Sbjct: 367 ALKTIVILT 375



>XP_005265401
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LKSTIVL 8
           LKST+VL
Sbjct: 376 LKSTLVL 382



>XP_005265400
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LKSTIVL 8
           LKST+VL
Sbjct: 389 LKSTLVL 395



>XP_005265399
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LKSTIVL 8
           LKST+VL
Sbjct: 407 LKSTLVL 413



>XP_006713335
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LKSTIVL 8
           LKST+VL
Sbjct: 420 LKSTLVL 426



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.126    0.285 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 259,021
Number of extensions: 204
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)