Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              19     869
NP_001035957                                                           19     869
XP_005276506                                                           19     869
XP_006721991                                                           19     869
XP_006721990                                                           19     869
XP_006721989                                                           19     869
XP_005258041                                                           19     869
XP_006721988                                                           19     869
XP_006721987                                                           19     869
XP_006721986                                                           19     869
XP_005258040                                                           19     869
XP_006721985                                                           19     869
XP_006720412                                                           19     869
NP_000758                                                              18    1935
XP_006723114                                                           18    1935
XP_005258626                                                           18    1935
XP_006723113                                                           18    1935
NP_001135920                                                           17    3301
NP_001135921                                                           17    3301
XP_006721159                                                           17    3301
XP_005255675                                                           17    3301
NP_001135923                                                           17    3301
NP_114427                                                              17    3301
NP_001135922                                                           17    3301
NP_001004684                                                           17    3301
NP_001004688                                                           17    4312
NP_001004691                                                           17    4312
NP_001004712                                                           17    4312
NP_001004689                                                           17    4312
NP_001005226                                                           16    5631
NP_036499                                                              16    5631
NP_065805                                                              16    7355
NP_001118230                                                           16    7355
XP_006721512                                                           16    7355
XP_006721511                                                           16    7355
XP_005247728                                                           16    7355
XP_006713777                                                           16    7355
NP_001004701                                                           16    7355
NP_001004742                                                           15    9605
XP_006723772                                                           15    9605
NP_542790                                                              15    9605
NP_001005480                                                           15    9605
NP_001004059                                                           15    9605
NP_001004727                                                           15    9605
NP_001005282                                                           15    9605
NP_001004700                                                           15    9605
NP_001004743                                                           15    9605
>NP_000258
          Length = 2818

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1565 ALKTLSIFY 1573
>NP_001035957
          Length = 2839

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1586 ALKTLSIFY 1594
>XP_005276506
          Length = 151

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ALKMLSIFY 9
           ALK LSIFY
Sbjct: 110 ALKTLSIFY 118
>XP_006721991
          Length = 1632

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1596 ALKTLSIFY 1604
>XP_006721990
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1596 ALKTLSIFY 1604
>XP_006721989
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1596 ALKTLSIFY 1604
>XP_005258041
          Length = 2836

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1565 ALKTLSIFY 1573
>XP_006721988
          Length = 2846

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1575 ALKTLSIFY 1583
>XP_006721987
          Length = 2849

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1596 ALKTLSIFY 1604
>XP_006721986
          Length = 2854

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1583 ALKTLSIFY 1591
>XP_005258040
          Length = 2857

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1586 ALKTLSIFY 1594
>XP_006721985
          Length = 2867

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ALKMLSIFY 9
            ALK LSIFY
Sbjct: 1596 ALKTLSIFY 1604
>XP_006720412
          Length = 128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  ALKMLSIFY 9
          ALK LSIFY
Sbjct: 87 ALKTLSIFY 95
>NP_000758
          Length = 491

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKMLSIFY 9
           LKML++FY
Sbjct: 196 LKMLNLFY 203
>XP_006723114
          Length = 386

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKMLSIFY 9
           LKML++FY
Sbjct: 196 LKMLNLFY 203
>XP_005258626
          Length = 422

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKMLSIFY 9
           LKML++FY
Sbjct: 196 LKMLNLFY 203
>XP_006723113
          Length = 457

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LKMLSIFY 9
           LKML++FY
Sbjct: 196 LKMLNLFY 203
>NP_001135920
          Length = 528

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           +MLSIFY
Sbjct: 186 RMLSIFY 192
>NP_001135921
          Length = 454

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           +MLSIFY
Sbjct: 164 RMLSIFY 170
>XP_006721159
          Length = 521

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           +MLSIFY
Sbjct: 231 RMLSIFY 237
>XP_005255675
          Length = 573

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           +MLSIFY
Sbjct: 231 RMLSIFY 237
>NP_001135923
          Length = 476

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           +MLSIFY
Sbjct: 186 RMLSIFY 192
>NP_114427
          Length = 528

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           +MLSIFY
Sbjct: 186 RMLSIFY 192
>NP_001135922
          Length = 455

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           +MLSIFY
Sbjct: 113 RMLSIFY 119
>NP_001004684
          Length = 330

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+S+FY
Sbjct: 288 KMISVFY 294
>NP_001004688
          Length = 347

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+S+FY
Sbjct: 272 KMVSVFY 278
>NP_001004691
          Length = 312

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+S+FY
Sbjct: 272 KMVSVFY 278
>NP_001004712
          Length = 310

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           K+LS+FY
Sbjct: 270 KLLSVFY 276
>NP_001004689
          Length = 312

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+S+FY
Sbjct: 272 KMVSVFY 278
>NP_001005226
          Length = 313

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+S+FY
Sbjct: 272 KMVSLFY 278
>NP_036499
          Length = 313

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+S+FY
Sbjct: 272 KMVSLFY 278
>NP_065805
          Length = 1444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALKMLSIFY 9
           A KML +FY
Sbjct: 447 APKMLGMFY 455
>NP_001118230
          Length = 549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   MLSIFY 9
           MLS+FY
Sbjct: 230 MLSVFY 235
>XP_006721512
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  MLSIFY 9
          MLS+FY
Sbjct: 60 MLSVFY 65
>XP_006721511
          Length = 398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  MLSIFY 9
          MLS+FY
Sbjct: 79 MLSVFY 84
>XP_005247728
          Length = 1413

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALKMLSIFY 9
           A KML +FY
Sbjct: 416 APKMLGMFY 424
>XP_006713777
          Length = 1424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ALKMLSIFY 9
           A KML +FY
Sbjct: 427 APKMLGMFY 435
>NP_001004701
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+++FY
Sbjct: 267 KMIAVFY 273
>NP_001004742
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+++FY
Sbjct: 270 KMVAVFY 276
>XP_006723772
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KMLSIF 8
           KMLSIF
Sbjct: 258 KMLSIF 263
>NP_542790
          Length = 696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KMLSIF 8
           KMLSIF
Sbjct: 264 KMLSIF 269
>NP_001005480
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KMLS+F+
Sbjct: 271 KMLSLFH 277
>NP_001004059
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+++FY
Sbjct: 267 KMVAVFY 273
>NP_001004727
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+++FY
Sbjct: 261 KMVAVFY 267
>NP_001005282
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+++FY
Sbjct: 271 KMVAVFY 277
>NP_001004700
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+++FY
Sbjct: 267 KMVAVFY 273
>NP_001004743
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   KMLSIFY 9
           KM+++FY
Sbjct: 270 KMVAVFY 276
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.149    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 267,339
Number of extensions: 262
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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